International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2022, 10(1), 1-11
DOI: 10.12691/ijebb-10-1-1
Open AccessArticle

Qualitative and Quantitative Assessment of a Surfactin biosurfactant in the Bioaugmentation of Crude-oil Contaminated Soil in Garages in the Republic of Congo

Paola Sandra Elenga Wilson1, Gabriel Judicael Okeni-Boba1, Christian Aimé Kayath1, , Kiele Molingo Mbemba1, Etienne Nguimbi1 and Gabriel Ahombo1

1Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien NGOUABI, BP. 69, Brazzaville, République du Congo

Pub. Date: June 30, 2022

Cite this paper:
Paola Sandra Elenga Wilson, Gabriel Judicael Okeni-Boba, Christian Aimé Kayath, Kiele Molingo Mbemba, Etienne Nguimbi and Gabriel Ahombo. Qualitative and Quantitative Assessment of a Surfactin biosurfactant in the Bioaugmentation of Crude-oil Contaminated Soil in Garages in the Republic of Congo. International Journal of Environmental Bioremediation & Biodegradation. 2022; 10(1):1-11. doi: 10.12691/ijebb-10-1-1

Abstract

The objective of this research was to perform a survey of different garages in seven (7) districts of the Republic of Congo and to further study the influence of biosurfactant produced by isolate M28 (Bacillus subtilis based on fib-E gene identification and RNA 16S gene sequences) and a consortium of selected bacteria on soil remediation. A total of 140 garages were found in Brazzaville. The evaluated soil samples showed that total petroleum hydrocarbons (TPH) content varied from 165 to 206 g/kg of soil. Unfortunately, in the Republic of Congo, the regulatory intervention values for TPH have yet to be determined. Four experiments were conducted in order to improve bioaugmentation. First experiment: wild contaminated soil and bacteria: The second experiment included wild contaminated soil, bacteria, and a biosurfactant; the third experiment included wild contaminated soil, a consortium, and a biosurfactant. Surfactin, a biorsurfactant produced by M28, was used to enhance bioaugmentation of wild contaminated soil from a garage from Bacongo.

Keywords:
Bacillus bioaugmentation biosurfactants garages

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