International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: http://www.sciepub.com/journal/ijdsr Editor-in-chief: Marcos Roberto Tovani Palone
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International Journal of Dental Sciences and Research. 2018, 6(3), 78-82
DOI: 10.12691/ijdsr-6-3-5
Open AccessCase Report

Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report

Abdul Aziz Aleid1,

1Conservative dental science department, college of dentistry, Qassim University, Buraidah, Kingdom of Saudi Arabia

Pub. Date: April 28, 2018

Cite this paper:
Abdul Aziz Aleid. Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report. International Journal of Dental Sciences and Research. 2018; 6(3):78-82. doi: 10.12691/ijdsr-6-3-5

Abstract

Aim: management of a clinical case with irreversible pulpitis for young adult molar with closed apices using analogous blood derivative PRF and evaluating the clinical and radiographic success for coronal pulpotomy. Summary: A 18 years old male patient reported to the screening clinic, was referred to endodontic clinic with acute pulpitis diagnosed in left first molar tooth. Deep occlusal caries involving pulp, with history of spontaneous lingering pain associated with sign and symptoms of pulpitis. Standard clinical care protocol was strictly implemented, rubber dam isolation was followed by coronal pulpotomy. Blood drawn from cuboidal vein. Platelet rich fibrin membrane placed over the remaining pulp and restored with MTA and finally with Glass ionomer cement. Follow-up results clinically and radio-graphically were encouraging and successful at day 1 to 24 months. No post-operative pain, the treated tooth responded to pulp sensibility test and radiographs revealed periapical healing. However, for standardization of the clinical procedure well designed research study is further needed.

Keywords:
pulpotomy tissue engineering pulpitis analogous PRF growth factor

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References:

[1]  He W, Wang Z, Luo Z, et al. LPS promote the odontoblastic differentiation of human dental pulp stem cells via MAPK signaling pathway. J Cell Physiol 2015; 230: 554-61.
 
[2]  He W, Qu T, Yu Q, et al. LPS induces IL-8 expression through TLR4, MyD88, NFkappaB and MAPK pathways in human dental pulp stem cells. Int Endod J 2013; 46:128-36. (2).
 
[3]  Ren G, Zhao X, Zhang L, et al. Inflammatory cytokine-induced intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in mesenchymal stem cells are critical for immunosuppression. J Immunol 2010; 184: 2321-8.
 
[4]  Jae-Hwan Kim, Su-Mi Woo, Nam-Ki Choi, Won-Jae Kim, Seon-Mi Kim,and Ji-Yeon Jung, Effect of Platelet-rich Fibrin on Odontoblastic Differentiation in Human Dental Pulp Cells Exposed to Lipopolysaccharide.
 
[5]  M. Abarajithan, N. Velmurugan, and D. Kandaswamy, “Management of recently traumatized maxillary central incisors by partial pulpotomy using MTA: case reports with two-year follow-up,” Journal of Conservative Dentistry, vol. 13, no. 2, pp. 110-113, 2010.
 
[6]  M. J. Eghbal, S. Asgary, R. A. Baglue, M. Parirokh, and J. Ghoddusi, “MTA pulpotomy of human permanentmolars with irreversible pulpitis,” Australian Endodontic Journal, vol. 35, no. 1, pp. 4-8, 2009.
 
[7]  Hess W (1929) Pulp amputation as a method of treating root canals. Dental Items Interest 51, 596-631.
 
[8]  S. Asgary and S. Ehsani, “Permanent molar pulpotomy with a new endodontic cement: a case series,” Journal of Conservative Dentistry, vol. 12, no. 1, pp. 31-36, 2009.
 
[9]  Burnouf, T., Su, C.-Y., Radosevich, M., Goubran, H. and El-Ekiaby, M. (2009), Blood-derived biomaterials: fibrin sealant, platelet gel and platelet fibrin glue. ISBT Science Series, 4: 136-142.
 
[10]  Choukroun J, Diss A, Simonpieri A, et al. (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 101, E56-60).
 
[11]  Kaigler D, Cirelli JA, Giannobile WV. Growth factor delivery for oral and periodontal tissue engineering. Expert Opin Drug Deliv 2006; 3: 647-62.
 
[12]  Shivashankar VY, Johns DA, Vidyanath S, Kumar MR. Platelet rich fibrin in the revitalization of tooth with necrotic pulp and open apex. J Conserv Dent 2012; 15: 395-8.
 
[13]  Langer R, Vacanti JP. Tissue engineering. Science.1993; 260 (5110): 920-6.
 
[14]  Kim, S.G.; Kahler, B.; Lin, L.M. Current developments in regenerative endodontics. Curr. Oral Health Rep. 2016, 3, 293-301.
 
[15]  Huang GT, Garcia-Godoy F (2014) Missing concepts in de novo pulp regeneration. J Dent Res 93: 717-724.
 
[16]  Ishizaka R, Hayashi Y, Iohara K, Sugiyama M,MurakamiM, Yamamoto T, Fukuta O, Nakashima M (2013) Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Biomaterials 34: 1888-1897.
 
[17]  Dohan Ehrenfest DM, Rasmusson L, Albrektsson T (2009) Classification of platelet concentrates: from pure pateletrich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 27: 158-167.
 
[18]  Lucarelli R, Beretta R, Dozza B et al. (2010) A recently developed bifacial platelet rich fibrin matrix. European cells & materials 20, 13-23.
 
[19]  Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. (2005) Differential growth factor retention by platelet rich plasma composites. Journal of oral and maxillofacial Surgery 63, 521-8.
 
[20]  Toffler M, Toscano N, Holtzclaw D, Del Corso M, Dohan Ehrenfest DM (2009) Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery milieu. The Journal of Implant and Advanced Clinical Dentistry 1, 21-31.
 
[21]  Tsai CH, Shen SY, Zhao JH, Chang YC. Platelet-rich fibrin modulates cell proliferation of human periodontally related cells in vitro. J Dent Sci 2009; 4: 130-5.
 
[22]  Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotech 2009; 27: 158-67.
 
[23]  Chang IC, Tsai CH, Chang YC. Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts [published online ahead of print July 8, 2010]. J Biomed Mater Res Part A cultures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 108: 341-52.
 
[24]  Witherspoon DE, Small JC, Harris GZ (2006) Mineral trioxide aggregate pulpotomies: a case series outcomes assessment. Journal of American Dental Association 137, 610-8)
 
[25]  M. J. Eghbal, S. Asgary, R. A. Baglue, M. Parirokh, and J.Ghoddusi, “MTA pulpotomy of human permanentmolars withirreversible pulpitis,” Australian Endodontic Journal, vol. 35, no.1, pp. 4-8, 2009.
 
[26]  P. Aguilar and P. Linsuwanont, “Vital pulp therapy in vital permanent teeth with cariously exposed pulp: a systematic review,” Journal of Endodontics, vol. 37, no. 5, pp. 581-587, 2011.
 
[27]  Ricucci D, Loghin S, Siqueira JF Jr (2014) Correlation between clinical and histologic pulp diagnoses. Journal of Endodontics 40, 1932-9.
 
[28]  Wang Z et al. (2010) Putative stem cells in human dental pulp with irreversible pulpitis: an exploratorystudy. Journal of Endodontics 36, 820-5.
 
[29]  Bakland LK, Andreasen JO. Will mineral trioxide aggregate replace calcium hydroxide in treating pulpal and periodontal healing complications subsequent to dental trauma? A review. Dental Traumatology 2012; 28: 25-32.
 
[30]  Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review-part II: leakage and biocompatibility investigations. Journal of Endodontics 2010; 36: 190-202.
 
[31]  Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-Part III: clinical applications, drawbacks, and mechanism of action. Journal of Endodontics 2010; 36: 400-13.