International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: http://www.sciepub.com/journal/ijdsr Editor-in-chief: Marcos Roberto Tovani Palone
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International Journal of Dental Sciences and Research. 2017, 5(4), 98-109
DOI: 10.12691/ijdsr-5-4-4
Open AccessArticle

Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants

Agenor Osório1, Roberto Rosendo Barros Reis2, Suzimara Reis Gea Osorio1, Adriana Neves Da Costa3, Karina Maria Salvatore Freitas4, and Marcelo Cavenaghi Pereira Da Silva2, 5

1Uninga University Center, Maringá, Brazil

2São Leopoldo Mandic, Campinas, Brazil

3Universidade Federal de São Paulo, Brazil

4Dentistry, Uninga University Center, Maringá, Brazil

5Morfofunctional Sciences, University of São Paulo, Brazil

Pub. Date: October 26, 2017

Cite this paper:
Agenor Osório, Roberto Rosendo Barros Reis, Suzimara Reis Gea Osorio, Adriana Neves Da Costa, Karina Maria Salvatore Freitas and Marcelo Cavenaghi Pereira Da Silva. Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants. International Journal of Dental Sciences and Research. 2017; 5(4):98-109. doi: 10.12691/ijdsr-5-4-4

Abstract

Midpalatine suture disjunction is a routine procedure in the orthodontic clinic; however, the cellular events involved in the procedure have not yet been completely explained. The aim of this study was to verify the tissue events, particularly those related to osteogenic differentiation, which occur in the region of the palatal suture after disjunction, by means of histologic and immunohistochemical analyses of the following noncollagenous proteins: BSP, OCC, OPN and ONC. In this study, 10 male Wistar rats aged two months and weighing approximately 250 g were used. The animals were divided into two groups of five animals: Group I – control (mini-implant placement) and group II – experimental (placement of mini-implant and disjunction spring made of 0.60mm steel wire, with an initial load of 2N (200 grams)). After 21 days, the animals were sacrificed and histological and immunohistochemical analyses were performed for the noncollagenous proteins. The use of the spring anchored to mini-implants promoted the disjunction of the median palatal suture, with a V-shaped opening, in which the base faced the superior region (at the nasal cavity) and the vertex was in the inferior region of the oral cavity (at the palatal bone). It could be concluded that after disjunction, there was presence of osteoblasts in an osteoid matrix in the region of the median palatal suture, and the immunohistochemical expression of the noncollagenous proteins increased in all the analyzed parameters, expression being most significant for ONC.

Keywords:
rapid maxillary expansion anchorage mini-implants noncollagenous proteins

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