International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: Editor-in-chief: Marcos Roberto Tovani Palone
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International Journal of Dental Sciences and Research. 2017, 5(3), 49-55
DOI: 10.12691/ijdsr-5-3-2
Open AccessArticle

Protective Effect of Chicken Egg Shell Powder Solution (CESP) on Artificially Induced Dental Erosion: An in Vitro Atomic Force Microscope Study

Sandleen Feroz1, , Faisal Moeen1 and Saleha Nisar Haq1

1Department of Dental Materials, Islamic International Dental College, Riphah University, Islamabad, Pakistan

Pub. Date: July 03, 2017

Cite this paper:
Sandleen Feroz, Faisal Moeen and Saleha Nisar Haq. Protective Effect of Chicken Egg Shell Powder Solution (CESP) on Artificially Induced Dental Erosion: An in Vitro Atomic Force Microscope Study. International Journal of Dental Sciences and Research. 2017; 5(3):49-55. doi: 10.12691/ijdsr-5-3-2


Aim: The aim of this in vitro study was to evaluate the effect of chicken egg shell powder solution (CESP) on the dental erosion produced by Coca Cola drink in permanent teeth. Material and methods: Thirty extracted human incisors were collected for this study purpose. After extraction all the specimens were cleaned and sectioned longitudinally resulting in 60 samples from a total of 30 tooth samples. All samples were randomly assigned to the following three groups. Group 1: Untreated group, Group 2: Demineralization by soft cola drink (4 cycles of 2 minutes’ immersion at 6 hours’ interval), Group 3: Demineralization by soft cola drink (4 cycles of 2 minutes’ immersion at 6 hours’ interval) followed by 7 days’ immersion in CESP. For surface roughness assessment images were taken from AFM. The root means square roughness (Rrms) was calculated from the AFM images. The differences in the mean roughness values between the three groups were analyzed by Anova and Tukey’s post hoc test. Results: One way Anova analysis revealed a statistically significant differences with p-value of 0.00, between the mean roughness values of three groups. Tuckey’s post hoc test revealed that there is a statistically significant difference (0.158±0.012) between the surface roughness of Group 2 and Group 3 as well as between Group 1 and Group 3(0.085±0.012) with a p-value of 0.00, indicating that following treatment by CESP the mean roughness values decreased significantly. Conclusion: CESP reduces the surface roughness of all treated teeth samples giving encorughment to the protective effect of CESP againts erosive enamel loss.

chicken egg shell powder solution (CESP) Enamel Demineralization Atomic Force Microscopy (AFM)

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