International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: Editor-in-chief: Marcos Roberto Tovani Palone
Open Access
Journal Browser
International Journal of Dental Sciences and Research. 2013, 1(2), 23-27
DOI: 10.12691/ijdsr-1-2-1
Open AccessArticle

Evaluation of Antibacterial Efficacy of Elexxion Diode Laser 810nm on the Infected Root Canals (In Vitro and In Vivo Study)

Njwan F. Shehab1, Zaid Adel Alshamaa2 and Mahmoud Y. M. Taha3,

1Department of Conservative Dentistry, College of Dentistry, Mosul University, Iraq

2Oral and Maxilla Facial Surgery, College of Dentistry, Mosul University, Iraq

3Department of Dental Basic Science, College Of Dentistry, Mosul University, Iraq

Pub. Date: October 09, 2013

Cite this paper:
Njwan F. Shehab, Zaid Adel Alshamaa and Mahmoud Y. M. Taha. Evaluation of Antibacterial Efficacy of Elexxion Diode Laser 810nm on the Infected Root Canals (In Vitro and In Vivo Study). International Journal of Dental Sciences and Research. 2013; 1(2):23-27. doi: 10.12691/ijdsr-1-2-1


This study investigated the antibacterial effect of Diode Laser (810nm) at different output powers in the root canals infected with Enterococcus faecalis in vitro and in vivo study. Root canal specimens were prepared from extracted, 50 single rooted teeth. After root canal preparation and sterilization, the root canals were infected with endodontic pathogens (Enterococcus faecalis). Following laser therapy, the canal contents were cultured on Enterococcus agar. The colony forming units recovered from each root canal was evaluated before and after laser treatment. This study also investigated antibacterial effect of laser in root canals associated with preiradicular lesions in vivo. In this in vivo study, 30 patients with infected root canals underwent diode laser treatment. To verify the findings, microbiological tests were performed and the results were computed. Result showed in vitro significant antibacterial difference between 1.05W, 1.5W, 1.95W and control group. In vivo there was no significant antimicrobial difference between untreated control group and 1.05W but significantly different from 1,5W and 1.95W in which there was no significant different between them.

disinfection 810nm diode root canal Enterococcus faecalis

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


Figure of 4


[1]  Guldener PHA, Langeland K., Aufbereitung und Reinigungdes Wuzrzelkanals. In: Endodontologie- Diagnostik und Therapie.’’ Thieme, Stuttgart/New York. 1992.
[2]  Moritz A., Gutknecht N., Schoop U., Goharkhay K., Doertbudak O. and Sperr W. Irradiation of Infected Root Canals With a Diode Laser In Vivo: Results of Microbiological Examinations. Lasers in Surgery and Medicine. 21. 221-226. 1997a.
[3]  Ulrich Schoop, Wolf Kluger, Andreas Moritz, Natascha Nedjelik, Wolfgang Sperr, Bactericidal Effect of Different Laser Systems In the Deep Layers of Dentin. Lasers in Surgery and Medicine. 35. 111-116. 2004.
[4]  Berutti E, Marini R, Angeretti A. Penetration ability of different irrigants into dentinal tubules. J Endod. 23(12). 725-727. 1997.
[5]  Distel JW, Hatton JF, Gillespie MJ. Biofilm formation inmedicated root canals. J Endod. 28(10).689-693. 2002.
[6]  Siqueira JF. Aetiology of root canal treatment failure: why welltreated teeth can fail. Int Endod J. 34. 1-10. 2001.
[7]  Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 85. 86-93. 1998.
[8]  Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J. 31. 1-7. 1998.
[9]  Heling I, Sommer M, Steinberg D, Friedman M, Sela MN. Microbiological evaluation of the efficacy of chlorhexidine in a sustained-release dexice for dentine sterilization. Int Endod J. 25. 15-9. 1992.
[10]  Bahcall J, Howard DVM, Miserendino L, Walis H. Preliminary investigation of the histological effects of laser endodontic treatment on the periradicular tissues in dogs. J Endod. 18. 47-51. 1992.
[11]  Moritz A, Gutknecht N, Goharkhay K, Schoop U, Wernisch J, Sperr, W. In vitro irradiation of infected root canals with a diode laser: Results of microbiologic, infrared spectrometric, and stain penetration examinations. Quintessence International. 28(3). 205-209. 1997b.
[12]  Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F. Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg. 18. 57-60. 2000.
[13]  Garcez AS, Núñez SC, Lage-Marques JL, Jorge AOC, and Ribeiro MS : Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 102(4). 93-98. 2006.
[14]  Jawetz E, Melnick, Adelbergs. Medical microbiology. 21st ed. Chapter 14. Stamford, Connecticut: Appleton & Lange 1998.
[15]  Nair PN. Pathogenesis of apical periodontitis and the causes of endodontic failures. Crit Rev Oral Biol Med. 15(6) 348-381. 2004.
[16]  Kouchi Y, Ninomiya J, Yasuda H, Fukui K, Moriyama T, Okamoto H.: Location of streptococcus mutans in the dentinal tubules of open infected root canals. J Dent Res. 59(12). 2038-2046. 1980.
[17]  Ulrich Schoop, Wolf Kluger, Selma Dervisbegovic, Kawe Goharkhay, Johann Wernisch, Apostolos Georgopoulos, Wolfgang Sperr, and Andreas Moritz: Innovative Wavelengths in Endodontic Treatment. Lasers in Surgery and Medicine. 38. 624-630. 2006.
[18]  Nair PNR, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after ‘one-visit’ endodontic treatment. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics. 99. 231-52. 2005.
[19]  Moritz A, Jakolitsch S, Goharkhay K, Schoop U, Kluger W, Mallinger R, Sperr W, Georgopoulos A. Morphologic changes correlating to different sensitivities of Escherichia coli and Enterococcus faecalis to Nd:YAG laser irradiation through dentin. Lasers Surg Med. 26: 250-261. 2000.
[20]  Hendy J. Newest 1064nm semiconductor lasers effectiveness in periodontics and endodontics. In: Abstracts, 5th SOLA Laser Congress. Vienna, Austria. J Oral Laser Appl. 9 (2/3). 154-155. 2009.
[21]  Nagwan Fadhel Shehab: Efficiency of Diode Laser and Sodium Hypochlorite as Root Canal Disinfectant Against Enterococcus faecalis: An In Vitro Study. M.Sc., Thesis. University of Mosul, Iraq. 2010.
[22]  Meire MA, De Prijck K, Coenye T, Nelis HJ and De Moor RJ. Antimicrobial efficacy of Nd:YAG and Er:YAG irradiation on Enterococcus faecalis biofilms on dentine Disks. J Oral Laser Appl. 9(2/3). 164-156. 2009.
[23]  Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT and Matsumoto K. Effect of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed laser Surg. 23(6). 575-581. 2005.
[24]  Gutknecht N, Franzen R, Schippers M, and Lampert F: Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg. 22(1). 9-13. 2004.
[25]  Gutknecht N, Moritz A, Conrads G, Sievert T, Sperr W, Lampert F. Bactericidal Effect of the Nd:YAG Laser in Vitro Root Canals. J Clin Las Med Surg. 14/2. 77-80. 1996.
[26]  Rooney J, Midda M, Leeming J. A laboratory investigation of the bactericidal effect of a Nd:YAG laser. Br Dent J.; 176. 61. 1994.
[27]  Silvana Jukić, Ivana Miletić, Želimir Božić, Ivica Anić, Dragica Snoljak and Smilija Kalenić. Antibacterial Effects of Nd:YAG Laser In Root Canals Samples: An In Vitro Study. Acta Clin Croat. 43. 3-7. 2004.
[28]  Silvana JukiE et al.: Antibacterial Effect of Er:YAG Laser in the Root Canal. Acta Stomatol Croat. 35. 201-204. 2001.
[29]  Wang QQ, Zhang CF, Yin, XZ. Evaluation of the bactericidal effect of Er,Cr:YSGG and Nd:YAG lasers in experimentally infected root canals. J Endod. 33. 830-832. 2007.
[30]  Garcez S., Silvia Cristina Nuñez, Michael R. Hamblin and Martha Simões Ribeiro. Antimicrobial Effects of Photodynamic Therapy on Patients with Necrotic Pulps and Periapical Lesion. J Endod. 34(2). 138-142. 2008.