International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: http://www.sciepub.com/journal/ijdsr Editor-in-chief: Marcos Roberto Tovani Palone
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International Journal of Dental Sciences and Research. 2016, 4(1), 10-16
DOI: 10.12691/ijdsr-4-1-3
Open AccessArticle

The Effect of Light Curing Units on Proliferation and Senescence of Human Dental Pulp Mesenchymal Stem Cells

Muhammet YALÇIN1, Halime KENAR2, Burak DAYI1, , Reyhan ŞİŞMAN1 and Erdal KARAÖZ3

1Department of Restorative Dentistry, Faculty of Dentistry, Inonu University, Malatya, Turkey

2Experimental and Clinical Research Center, Kocaeli University, Kocaeli, Turkey

3Liv Hospital Head of Department Center for Regenerative Medicine and Stem Cell Research & Manufacturing, İstanbul, Turkey

Pub. Date: February 25, 2016

Cite this paper:
Muhammet YALÇIN, Halime KENAR, Burak DAYI, Reyhan ŞİŞMAN and Erdal KARAÖZ. The Effect of Light Curing Units on Proliferation and Senescence of Human Dental Pulp Mesenchymal Stem Cells. International Journal of Dental Sciences and Research. 2016; 4(1):10-16. doi: 10.12691/ijdsr-4-1-3

Abstract

The aim of this study was to evaluate the effect of different dental light curing units on cellular senescence and proliferation rate of human dental pulp mesenchymal stem cells (hDP-MSCs). hDP-MSCs were seeded at a density of 1x104 cells/cm2 into a 96 well plate. Cells were allowed to attach for 24 h and Halogen curing unit, Light-Emitting-Diode and Plasma arc units (PAC-MOD1, PAC-MOD2, PAC-MOD3) were applied on the cells from 1.2 cm distance (8 mm air + 4 mm growth medium). Media of the wells was refreshed after irradiation and cells cultured at 37 oC in a 5% CO2 incubator for 48 h. Cell growth was determined using the WST-1 cell proliferation assay. The same samples were fixed and evaluated for cellular senescence, the irreversible growth arrest of cells, by staining for SA-β-galactosidase activity. As statistical analyses, Mann-Whitney U test was used for senescence data evaluation and One-way Anova and Tukey HSD tests were used for proliferation data evaluation. Cell proliferation rate was significantly higher under PAC-MOD3 conditions than under Halojen curing unit and PAC-MOD1( p< 0.05). According to the senescence test results, there was no statistically significant difference between the experimental groups and the control group ( p > 0.05), but senescence of hDP-MSCs exposed to PAC-MOD2 was greater than the others. When the dental light curing units are used for polymerization of adhesive systems in pulp capping, this procedure may have effects on senescence and proliferation of dental pulp stem cells.

Keywords:
light curing units dental pulp mesenchymal stem cells cellular senescence cellular proliferation

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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References:

[1]  Domingos, P. A., Garcia, P. P., de Oliveira, A. L. and Palma-Dibb, R. G, “Composite resin color stability: influence of light sources and immersion media,” J Appl Oral Sci, 19 (3), 204-211, 2011.
 
[2]  Cavalcanti, B. N., Rode Sde, M., Franca, C. M. and Marques, M. M, “Pulp capping materials exert an effect on the secretion of IL-1beta and IL-8 by migrating human neutrophils,” Braz Oral Res, 25(1), 13-18, 2011.
 
[3]  Uhl, A., Volpel A. and Sigusch, B. W, “Influence of heat from light curing units and dental composite polymerization on cells in vitro,” J Dent, 34 (4), 298-306, 2006.
 
[4]  Aguiar, F. H., Braceiro, A., Lima, D. A., Ambrosano, G. M. and Lovadino, J. R, “Effect of light curing modes and light curing time on the microhardness of a hybrid composite resin,” J Contemp Dent Pract, 8 (6), 1-8, 2007.
 
[5]  Hussey, D. L., Biagioni, P. A. and Lamey, P. J, “Thermographic measurement of temperature change during resin composite polymerization in vivo,” J Dent, 23 (5), 267-271, 1995.
 
[6]  Shortall, A. C. and Harrington, E, “Temperature rise during polymerization of light-activated resin composites,” J Oral Rehabil, 25 (12), 908-913, 1998.
 
[7]  Hannig, M. and Bott, B, “In-vitro pulp chamber temperature rise during composite resin polymerization with various light-curing sources,” Dent Mater, 15 (4), 275-281, 1999.
 
[8]  Stevens, A., Zuliani, T., Olejnik, C. et al., “Human dental pulp stem cells differentiate into neural crest-derived melanocytes and have label-retaining and sphere-forming abilities,” Stem Cells Dev, 17 (6), 1175-1184, 2008.
 
[9]  Goldberg, M. and Smith, A. J, “Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis for Repair and Tissue Engineering,” Crit Rev Oral Biol Med, 15 (1), 13-27, 2004.
 
[10]  Yoshiba, K., Yoshiba, N., Nakamura, H., Iwaku, M. and Ozawa, H, “Immunolocalization of fibronectin during reparative dentinogenesis in human teeth after pulp capping with calcium hydroxide,” Journal of dental research, 75 (8), 1590-1597, 1996.
 
[11]  Morsczeck, C., Gotz, W., Schierholz, J. et al., “Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth,” Matrix Biol, 24 (2), 155-165, 2005.
 
[12]  Karaoz, E., Dogan, B. N., Aksoy, A. et al., “Isolation and in vitro characterisation of dental pulp stem cells from natal teeth,” Histochem Cell Biol, 133(1), 95-112, 2010.
 
[13]  Gronthos, S., Mankani, M., Brahim, J., Robey, P. G. and Shi, S, “Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo,” Proc Natl Acad Sci U S A, 97 (25), 13625-13630, 2000.
 
[14]  Bahrololoomi, Z., Soleimani, A. A., Jafari, N. and Varkesh, B, “Micro-leakage of a Fissure Sealant Cured Using Quartz-tungsten-halogen and Plasma Arc Light Curing Units,” J Dent Res Dent Clin Dent Prospects,8(4), 252-255, 2014.
 
[15]  Ergun, G., Egilmez, F. and Yilmaz, S, “Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led,” J Appl Oral Sci, 19 (3), 286-292, 2011.
 
[16]  Mills, R. W., Jandt, K. D. and Ashworth, S. H, “Dental composite depth of cure with halogen and blue light emitting diode technology,” Br Dent J, 186 (8), 388-391, 1999.
 
[17]  Thind, B. S., Stirrups, D. R. and Lloyd, C. H, “A comparison of tungsten-quartz-halogen, plasma arc and light-emitting diode light sources for the polymerization of an orthodontic adhesive,” Eur J Orthod, 28 (1), 78-82, 2006.
 
[18]  Tissieres, A., Mitchell, H. K. and Tracy, U. M, “Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs,” J Mol Biol, 84 (3), 389-398, 1974.
 
[19]  Yap, aA. U. and Soh, M. S, “Thermal emission by different light-curing units,” Oper Dent, 28 (3), 260-266, 2003.
 
[20]  Taher, N. M., Al-Khairallah, Y., Al-Aujan, S. H. and Ad'dahash, M, “The effect of different light-curing methods on temperature changes of dual polymerizing agents cemented to human dentin,” J Contemp Dent Pract, 9 (2), 57-64, 2008.
 
[21]  Knezevic, A., Zeljezic, D., Kopjar, N. and Tarle, Z, “Cytotoxicity of composite materials polymerized with LED curing units,” Oper Dent, 33 (1), 23-30, 2008.
 
[22]  van Breugel, H. H. and Bar, P. R, “Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro,” Lasers Surg Med, 12 (5), 528-537, 1992.
 
[23]  Wataha, J. C.,. Lockwood, P. E., Lewis, J. B., Rueggeberg, F. A. and Messer, R. L, “Biological effects of blue light from dental curing units,” Dent Mater, 20(2), 150-157, 2004.
 
[24]  Yoon, T. H., Lee, Y. K., Lim, B. S. and Kim, C. W, “Degree of polymerization of resin composites by different light sources,” J Oral Rehabil, 29 (12), 1165-1173, 2002.
 
[25]  Gorgidze, L. A., Oshemkova, S. A. and Vorobjev, I. A, “Blue light inhibits mitosis in tissue culture cells,” Biosci Rep, 18 (4), 215-224, 1998.
 
[26]  Aggarwal, B. B., Quintanilha, A. T., Cammack, R. and Packer, L, “Damage to mitochondrial electron transport and energy coupling by visible light,” Biochim Biophys Acta, 502 (2), 367-382, 1978.
 
[27]  Callaghan, G. A., Riordan, C., Gilmore, W. S., McIntyre, I. A., Allen, J. M. and Hannigan, B. M, “Reactive oxygen species inducible by low-intensity laser irradiation alter DNA synthesis in the haemopoietic cell line U937,” Lasers Surg Med, 19 (2), 201-206, 1996.
 
[28]  Alvarez, S. and Boveris, A, “Induction of antioxidant enzymes and DT-diaphorase in human blood mononuclear cells by light stress,” Arch Biochem Biophys, 305 (2), 247-251, 1993.