International Journal of Dental Sciences and Research
ISSN (Print): 2333-1135 ISSN (Online): 2333-1259 Website: Editor-in-chief: Marcos Roberto Tovani Palone
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International Journal of Dental Sciences and Research. 2016, 4(1), 1-4
DOI: 10.12691/ijdsr-4-1-1
Open AccessArticle

Comparison of the Antibacterial Efficacy of Several Dentin Bonding Agents: Two Different in Vitro Studies

Mustafa Duzyol1, , Murat Karamese2, Nilgun Akgul3, Pinar Gul3, Neslihan Celik3, Esra Duzyol4 and Ulku Altoparlak5

1School of Dentistry, Department of Restorative Dentistry, Gaziantep University, 27000 Gaziantep, Turkey

2School of Medicine, Department of Microbiology, Kafkas University, 36000 Kars, Turkey

3School of Dentistry, Department of Restorative Dentistry, Ataturk University, 25240 Erzurum, Turkey

4School of Dentistry, Department of Pediatric Dentistry, Ataturk University, 25240 Erzurum, Turkey

5School of Medicine, Department of Microbiology, Ataturk University, 25240 Erzurum, Turkey

Pub. Date: January 18, 2016

Cite this paper:
Mustafa Duzyol, Murat Karamese, Nilgun Akgul, Pinar Gul, Neslihan Celik, Esra Duzyol and Ulku Altoparlak. Comparison of the Antibacterial Efficacy of Several Dentin Bonding Agents: Two Different in Vitro Studies. International Journal of Dental Sciences and Research. 2016; 4(1):1-4. doi: 10.12691/ijdsr-4-1-1


Objective: In this study, we compared the antibacterial effects of several dentin bonding agents with different pH values and active monomers. Method and Materials: Infected dentin samples were obtained from depths of approximately 4-6 mm, from isolated and air-dried dental caries. Streptococcus mitis was isolated and incubated on sheep blood agar plates at 37°C for 18 h. Impregnated antimicrobial disks were used for the liquids, and the samples were grouped as follows: group 1 was a negative control group with nothing applied; group 2 was a positive control group with 2% chlorhexidine digluconate applied; group 3 had Adper Single Bond Universal applied; group 4 had Clearfil SE Bond 2 (including 10-methacryloyloxydodecyl dihydrogen phosphate[MDP]) applied; group 5 had Clearfil S3 Bond Plus (including [MDP])applied; and group 6 had Clearfil Protect Bond (including 12-methacryloyloxydodecylpyridinium bromide [MDPB])applied. The antimicrobial disks were inserted into the blood agar plates. Inhibition zones were measured on the plates by well-educated specialists. Results: The 2% chlorhexidine digluconate solution had a more extensive inhibition zone than the other groups. S. mitis was significantly inhibited on the MDPB-impregnated disks applied to the agar plates. Conclusions: The results demonstrated that dentin bonding agents including MDPB-containing primer had significant antibacterial effects.

dentin bonding agents MDPB antimicrobial effect agar-disk diffusion tryptophan-containing broth

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