International Journal of Celiac Disease
ISSN (Print): 2334-3427 ISSN (Online): 2334-3486 Website: Editor-in-chief: Samasca Gabriel
Open Access
Journal Browser
International Journal of Celiac Disease. 2018, 6(3), 87-88
DOI: 10.12691/ijcd-6-3-2
Open AccessArticle

False-negative IgA Anti-tissue Transglutaminase

Nada Boutrid1, 2, , Hakim Rahmoune1, 2 and Mounira Amrane2, 3

1Department of Pediatrics, University Hospital of Setif; Setif 1 University, Algeria

2Genetic, Cardiovascular & Nutritional Diseases Laboratory; Setif-1 University, Algeria

3Central Laboratory, CAC Hospital of Setif; Setif-1 University, Algeria

Pub. Date: January 04, 2019

Cite this paper:
Nada Boutrid, Hakim Rahmoune and Mounira Amrane. False-negative IgA Anti-tissue Transglutaminase. International Journal of Celiac Disease. 2018; 6(3):87-88. doi: 10.12691/ijcd-6-3-2


The expanding knowledge about gluten-related autoimmunity led to a serology-based step-wised approach to diagnose celiac disease that might be misleading due to laboratory techniques. We present a rare case associating initial false-negative anti-transglutaminase IgA antibodies while CD was confirmed by IgG anti-transglutaminase and subsequent duodenal biopsy.

celiac disease autoimmunity immunoglobulin A tissue transglutaminase

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Hill ID, Dirks MH, Liptak GS, et al. Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr 2005; 40: 1-19.
[2]  Rahmoune H,Boutrid N,Bioud B. HLA & Celiac Disease. Moroccan Health Journal, 2017; 18 :14-15.
[3]  Catassi C, Ratsch IM, Gandolfi L, Pratesi R, Fabiani E, El Asmar R, Frijia M, Bearzi I, Vizzoni L. Why is coeliac disease endemic in the people of the Sahara?.The Lancet. 1999; 354: 647-8.
[4]  Barada K, Bitar A, Mokadem MA, Hashish JG, Green P. Celiac disease in Middle Eastern and North African countries: a new burden? World J Gastroenterol 2010; 16:1449-1457.
[5]  Boudraa G, Hachelaf W, Benbouabdellah M, Belkadi M, Benmansour FZ, Touhami M. Prevalence of coeliac disease in diabetic children and their first‐degree relatives in West Algeria: screening with serological markers. Acta paediatrica. 1996; 85: 58-60.
[6]  Megiorni F, Mora B, Bonamico M, Barbato M, Montuori M, Viola F, Trabace S, Mazzilli MC. HLA-DQ and susceptibility to celiac disease: evidence for gender differences and parent-of-origin effects. The American journal of gastroenterology. 2008; 103: 997-1003.
[7]  Ivanovski P, Nikolić D, Dimitrijević N, Ivanovski I, Perišić V. Erythrocytic transglutaminase inhibition hemolysis at presentation of celiac disease. World Journal of Gastroenterology: WJG. 2010 Nov 28; 16(44): 5647.
[8]  Arguelles-Grande C, Norman GL, Bhagat G, Green PH. S2054 Hemolysis Induces Tissue Transglutaminase, but Not Deamidated Gliadin Peptide, False Negative Results in Celiac Disease Patients With Low Antibody Levels. Gastroenterology. 2010 May 1; 138(5): S-310.
[9]  Arguelles-Grande C, Norman GL, Bhagat G, Green PH. Hemolysis Interferes with the Detection of Anti–Tissue Transglutaminase Antibodies in Celiac Disease. Clinical chemistry. 2010 Jun 1;56(6): 1034-6.
[10]  Wolf J, Haendel N, Remmler J, Kutzner CE, Kaiser T, Mothes T. Hemolysis and IgA‐antibodies against tissue transglutaminase: When are antibody test results no longer reliable?. Journal of clinical laboratory analysis. 2018 May 1:e22360.
[11]  Eriksen F, Charoenpatrawut J, Zaman S, Thomasen IN, Bathum L. Hemolysis may cause false negative results and underdiagnosis of celiac disease when measuring anti-tissue transglutaminase antibodies in serum by immunoassays. Scandinavian journal of clinical and laboratory investigation. 2018 May 14: 1-2.