International Journal of Celiac Disease
ISSN (Print): 2334-3427 ISSN (Online): 2334-3486 Website: http://www.sciepub.com/journal/ijcd Editor-in-chief: Samasca Gabriel
Open Access
Journal Browser
Go
International Journal of Celiac Disease. 2017, 5(4), 159-163
DOI: 10.12691/ijcd-5-4-8
Open AccessArticle

Characterization of Enterobacteries Genus in Intestinal Microbiota of Celiac Children

Fatima Lahcene1, , Aicha Tir Touil Meddah1, Karim Bouziane-Nedjadi2, Boumediene Meddah1 and André Leke3

1Laboratory of Bioconversion Microbiological Engineering and Sanitary Safety (LBMESS), University of Mascara, Algeria

2Department of pediatrics “C” (A. Cabral), CHU of Oran, Algeria

3Department of pediatric resuscitation, CHU, Amiens, France

Pub. Date: November 24, 2017

Cite this paper:
Fatima Lahcene, Aicha Tir Touil Meddah, Karim Bouziane-Nedjadi, Boumediene Meddah and André Leke. Characterization of Enterobacteries Genus in Intestinal Microbiota of Celiac Children. International Journal of Celiac Disease. 2017; 5(4):159-163. doi: 10.12691/ijcd-5-4-8

Abstract

The imbalance of the intestinal microbiota is link, by several diseases such as celiac disease, which causes inflammation in the small intestine. This inflammation is due by the digestion of gluten present in some type of cereals. In this work, fecal and duodenal biopsy samples were collected to characterize by conventional culture technique the composition of the Enterobacteries group in intestinal mirobiota of celiac children and were compared with control children. A significant difference detected in the intestinal flora of celiac children compared to controls children concerning the Enterobacteries group. We found an increase of E.coli, Enterobacter aerogeneses, and Klebsiella with presence of Salmonella sp, Shigella sp in biopsy and fecal samples of celiac children and a relationship between the increase of Enterobacter cloacea and the presence of positive anti-transglutaminase value.

Keywords:
celiac disease intestinal microbiota enterobacteries E.coli anti-transglutaminase

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Cano N, Barnoud D, et Hassalmannet M. Adult Artificial Nutrition Treatment.Sringer, Paris. 2007
 
[2]  Corthier. Intestinal flora and health: what issues?. 47th Annual nutrition and diététique- day "Symposium: intestinal flora and health. CNIT Paris. Elsevier Masson SAS. 2007; 76.
 
[3]  Hooper LV and Gordon J I. Commensal host-bacterial relationships in the gut .Science. 2001; 292(5519): 1115-8.
 
[4]  Delzenne. M, Cani. D. Involvement of the intestinal flora in the energy metabolism. Revue scientifique MEDECINE/SCIENCES. 2008; 24 : 505-10.
 
[5]  Sekirov I, Russell SL, L Antunes LC, and Finlay BB. Gut microbiota in health and disease.Physiological Reviews . 2010; 90(3): 859-904.
 
[6]  Goulet O. The intestinal flora: a living mode to preserve. Journal of pediatrics and nursery 2009; 22:102-106.
 
[7]  Hopkins MJ et Macfarlane GT. Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection. J Med Microbiol. 2002 51(5): 448-54.
 
[8]  Guandalini S, Setty M. Celiac disease. CurrOpinGastroenterol. 2008 Nov. 24(6): 707-12.
 
[9]  Mulder CJ, and Bartelsman JF. Case-finding in celiac disease should be intensified.Best Pract Res Clin Gastroenterol. 2005; 19: 479-486.
 
[10]  Rewers M. Epidemiology of celiac disease: what are the prevalence, incidence, andprogression of celiac disease? Gastroenterology. 2005; 128: 47-51.
 
[11]  Cegarra M. The gluten-free diet: difficulties with follow-up. Archives of Pediatrics. 2006; 13: 576-578.
 
[12]  Schmitz J. Gluten-free diet in children. Journal of Pediatrics and Childcare. 2007; 20: 337-344.
 
[13]  Collado. MC, Sanz Y. Quantification of mucosa-adheredmicrobiota of lambs and calves by the use of culture methods and fluorescent in situ hybridization coupled with flow cytometry techniques. Vet Microbiol . 2007; 121: 299-306.
 
[14]  Béraud M. The technician biological analyzes theoretical and practical guide.TeDc Lavoisier. 2001; 988-990.
 
[15]  Balows A, Hausler W, Herrmann K, Isenberg H, Shadomy H. Manual of clinical microbiology. Washington (DC): ASM. 1991
 
[16]  Collado C ,Donat E, Ribes-Koninck C, Calabuig M, Sanz Y. Specific duodenal and faecal bacterial groups associated with paediatric coeliac disease. JCP. 2009; 62(3): 264-9.
 
[17]  Hopper AD, Cross SS, Sanders DS.Patchy villous atrophy in adult patients with suspected gluten- sensitive enteropathy: is a multiple duodenal biopsy strategy appropriate? Endoscopy. 2008; 40(3): 219-224.
 
[18]  Sánchez E, Donat E, Ribes-Koninckx C,Fernández-MurgaM , and Sanz Y..Duodenal-Mucosal Bacteria Associated with Celiac Disease in Children. Appl Environ Microbiol. 2013; 79(18): 5472.
 
[19]  Björkstén B, Sepp E, Kulge, J, and al. Allergy development and the intestinal microflora during the first year of life. J Allergy Clin Immunol. 2001; 108(4): 516-20
 
[20]  Schippa S, Iebba V, Barbato M, Di Nardo G,Totino V, Checchi MP, Longhi C,Maiella G, Cucchiara S, Conte, MP. A distinctive ‘microbial signature’ in celiac pediatric patients.BMC Microbiol. 2010; 10, 175.
 
[21]  Schippa S, Totino V,Marazzato M, Lepanto M, Santangelo F., Aleandri M., Gagliardi A., Longhi C., Pantanella, F., Iebba V., and al. Escherichia coli population-based study in pediatric crohn’s disease. Adv. Microbiol. 2014, 4, 886-889.
 
[22]  Darfeuille-Michaud A, Boudeau J, Bulois P, Neut C., Glasser AL, Barnich N, Bringer MA, Swidsinski A, Beaugerie L. Colombel JF. High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn’s disease. Gastroenterology.2004; 127: 412-421.
 
[23]  Conte MP, Schippa S, Zamboni I, Penta M., Chiarini F, Seganti L, Osborn J, Falconieri P, Borrelli O, et Cucchiara S.Gut-associated bacterial microbiota in pediatric patients with inflammatorybowel disease. Gut. 2006; 55(12):1760-7.
 
[24]  Collado C, Donat E, Ribes-Koninck C, Calabuig M, Sanz. Specific duodenal and faecal bacterial groups associated with paediatric coeliac disease .JCP. 2009; 62(3): 264-9.
 
[25]  Di Cagno R, Rizzello CG, Gagliardi F., Ricciuti P, Ndagijimana M, Francavilla R., Guerzoni ME, Crecchio C, Gobbetti M., et De Angelis M. Different fecal microbiotas and volatile organic compounds in treated and untreated children with celiac disease. Appl Environ Microbiol. 20097; 5: 3963-3971.
 
[26]  Sánchez E, Nadal I, Donat E, Ribes-Koninckx C, Calabuig M, Sanz Y.. Reduced diversity and increased virulence-gene carriage in intestinalenterobacteria of coeliac children. BMC Gastroenterol. 2008; 8(50): 10.
 
[27]  Baron S. Health and Micro nutrition: A global approach adapted to the pharmacy in adults. Doctoral thesis in pharmacy.Universityof Rouen. 2014; 187.
 
[28]  Verdu EF, Galipeau HJ, et Jabri B. Novel players in coeliac disease pathogenesis: role of the gut microbiota. Nat Rev Gastroenterol Hepatol (sous presse). 2015.
 
[29]  Caja S, Myrsky E, Korponay-Szabo IR, Nadalutti C, Sulic AM, Lavric M, Sblattero D, Marzari R, Collighan R, Mongeot A, Griffin M, Mäki M, Kaukinen K, et Lindfors K. Inhibition of transglutaminase 2 enzymatic activity ameliorates the anti-angiogenic effects of coeliac disease autoantibodies. Scand J Gastroenterol. 2010; 45: 421-427.
 
[30]  De Sousa Moraes LF, Grzeskowiak LM, de Sales Teixeira TF, et Gouveia Peluzio Mdo C. Intestinal Microbiota and Probiotics in Celiac Disease. Clin Microbiol Rev. 2014; 27:482-9.