Isolation and Laboratory Diagnosis of Antibiotic-resistant E. coli from Surgical Wounds of Inpatients at Zagazig University Hospitals, Egypt

Saly M. E. Toubar¹, Mahmoud M. M. Zaky¹ and Ahmed Samy El-Shafey^2,

¹Botany Department, Faculty of Science, Port-Said University, Port-Said, Egypt

²Microbiology Section, Faculty of Science, Tanta University, Tanta, Egypt

Cite this paper:
Saly M. E. Toubar, Mahmoud M. M. Zaky and Ahmed Samy El-Shafey. Isolation and Laboratory Diagnosis of Antibiotic-resistant E. coli from Surgical Wounds of Inpatients at Zagazig University Hospitals, Egypt. International Journal of Celiac Disease. 2020; 8(4):143-146. doi: 10.12691/ijcd-8-4-7

Abstract

E. coli is one of the most common community and nosocomial pathogens and it mainly causes skin and soft tissue infections. So, the current study aimed to study the isolation of antibiotic-resistant E.coli strains in Egypt, Hospitals. Study their antibiotic susceptibility profile and therapy to control and present the transmission of E. coli strains among the health care settings.120 pus samples were collected from post-operation wounds of a study group of inpatients in Egypt, Hospitals. Bacteria colonizing the wounds were isolated on specific culture media thereafter purified and divided into 4 groups depending on their morphological characters and Gram's stain reaction Isolated bacteria were divided into 4 groups. Group (I) represented 40% of total isolates; while group (II) & (III) represented 25% and group (IV) 22%. Isolated bacteria related to groups I, II, III & IV were preliminary identified as S. aureus. E. coli, K. pneumoniae and P. aeruginosa, respectively. Antibiotic susceptibility of the 4 groups of isolated bacteria against 10 different antibiotics revealed that Ciprofloxacin showed the highest activity against E. coli. Data revealed that strain encoded E.coli 6 is a multi-resistant strain as it resisted 13 antibiotics out of 15 (86.7%).

Keywords:
E. coli nosocomial pathogens wounds ciprofloxacin vancomycin

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Anguzu, J.R. and Olila, D. (2006). Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a referral hospital in Uganda. Afr HealthSci. 7(3): 148-154.
[2]	Chanal, C.; Sirot, D.; Romaszko, J. P.; Bret, L. and Sirot, J. (2010). Survey of prevalence of extended spectrum (3-Lactamases among exterobacteriaceae. J. Antimicrob. Chemother. 38: J27-132.
[3]	Arias, C .A and Barbara, E. Murray B. E (2012). The rise of the Enterococcus: beyond vancomycin resistance. Nat Rev Microbiol. 2012 Mar 16.
[4]	Srinivasan, A.; Dick and Trish M. (2011). Vancomycin Resistance in Staphylococci Clin Microbial Rev. 1 5(3): 430-138.
[5]	Bauer, A.W.; Kirby, W.M.; Sherries, J.C. and Truck, M. (2009). Antibiotic susceptibility testing by the standard single disk method, J.Clin. pathol., 45: 493-496.
[6]	Washington, J. A. and Sutter, V. L. (2012). Dilution susceptibility test, Agarand macro broth dilution procedures. J. Med. Microbiol. 10: 453-458.
[7]	Chastre J, Trouillet. JL. (2007). Problem pathogens (Psendomonasaeruginosa and Acinetobacter). SeminRespir Infect 2000; 15: 287-98.
[8]	Abu Hanifah Y. (2012). Post-operative surgical wound infection. Med. J. Malaysia 2001; 45: 293-7.
[9]	Giacommetti, A.; Ciriani O.; Schimizzi, A.M.; Del prete M.S.; Barchiesi, F.; Derrico M, M.; petrelli E. and Scalise G. (2009). Epidemiology andMicrobiology of surgical wound infections.J. Clin.Microb. 38(2): 918.
[10]	Malone DL, Genuit T, Traey, JK, Gannon C, Napolitano LM (2011). Surgical site infections. Reanalysis of risk Factors. J Surg Res 2002; 103: 89-05.
[11]	Thanni LO, Osinupebi OA, Deji-Agboola M. (2008). Prevalence of bacterial pathogens in infected wounds in a tertiary hospital, 1995-2001: any change in trend? J. Natl Med Assoc 2003; 95: 1189-95.
[12]	Surucuoglu, S., Gazi, H., Kurutepe, S., Ozkutuk, N. and Ozbakkaloglu, B. (2006). Bacteriology of Surgical Wound Infections in a Tertiary Care Hospital in Turkey. East Afr. Med . J., 82(7): 331-336.
[13]	Mulu A, Moges F, Tessemn B, Kassu A. (2011). Pattern and multiple drug resistance of bacterial pathogens isolated from wound infection at University of Gondar Teaching Hospital, North West Ethiopia. Ethiop Med. J. 44(2): 125-131.
[14]	Zaid, A. M. (2011). Studies on β-lactamascs producing bacteria belonging to genus Pseudomonas. Ph.D. thesis. Facultyof science, Zigzaguniversity
[15]	Gowan, J. F; Jr Hall, E. C. and Parrott, P. L. (2009). Antimicrobial susceptibility in Gram-negative bacteremia are nosocomial isolates more resistant. Antimicrobial Agents Chemotherapy. 39 (11): 645-649.
[16]	Amyes, G. B. and Gemmell, C.G. (2007). Antibiotic resistance. J. Med. Microbial. 46 (2): 436-470.
[17]	Hall, L. M.; Blok, M.; Dondcrs, T.; Paauw, A.; Fluit, A. C. and Verhoef, J. (2008). Multidrug resistance among Enterobacteriacae is strongly associated with the presence of integrons and is independent of species or isolate origin .J. Infact. Dis., 187(2): 251-259.