American Journal of Environmental Protection
ISSN (Print): 2328-7241 ISSN (Online): 2328-7233 Website: http://www.sciepub.com/journal/env Editor-in-chief: Mohsen Saeedi, Hyo Choi
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American Journal of Environmental Protection. 2016, 4(2), 38-47
DOI: 10.12691/env-4-2-1
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Heavy Metals in Epiphytic Lichens and Mosses of Oil–Producing Communities of Eket and Ibeno, Akwa Ibom State – Nigeria

Aniefiok E. Ite1, 2, , Uwem U. Ubong1, Usoro M. Etesin1, Edet W. Nsi1, Emmanuel J. Ukpong1, Akanimo N. Ekanem1, Usenobong F. Ufot3 and Anietimfon I. Udo1

1Department of Chemistry, Akwa Ibom State University, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria

2Research and Development, Akwa Ibom State University, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria

3Department of Biological Science, Akwa Ibom State University, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria

Pub. Date: July 05, 2016

Cite this paper:
Aniefiok E. Ite, Uwem U. Ubong, Usoro M. Etesin, Edet W. Nsi, Emmanuel J. Ukpong, Akanimo N. Ekanem, Usenobong F. Ufot and Anietimfon I. Udo. Heavy Metals in Epiphytic Lichens and Mosses of Oil–Producing Communities of Eket and Ibeno, Akwa Ibom State – Nigeria. American Journal of Environmental Protection. 2016; 4(2):38-47. doi: 10.12691/env-4-2-1

Abstract

Epiphytic lichen (Parmelia carperata) and moss (Polytrichum juniperinum, Calymperes erosum and Racopilum africanum) samples were used as bioindicators and bioaccumulators of atmospheric heavy metals deposition in oil–producing host communities of Eket and Ibeno Local Government Areas of Akwa Ibom State – Nigeria. Sampling of lichen and moss species that are found to grow extensively and abundantly on the stems and branches of several plants was performed during September 2014 at 25 sampling location sevenly distributed over the two oil–producing host communities studied. Unwashed, oven dried and homogenized powdered lichen and moss samples were mineralized using wet digestion with 3:1 mixture of concentrated nitric acid and perchloric acid in Teflon beakers on a Gerhardt digestion hot plate. The concentrations of heavy metals and/or trace elements were determined by atomic absorption spectrometry (AAS) equipped with flame and/or graphite furnace systems. The concentration of heavy metals in lichen and moss samples ranged from 0.003 – 0.009 μg g−1 for Cadmium (Cd); 0.006 – 7.654 μg g−1 for Chromium (Cr); 1.120 – 1.999 μg g−1 for Cobalt (Co); 8.954 – 116.760 μg g−1 for Copper; 25.980 – 193.260 μg g−1 for Manganese (Mn); 2.268 – 23.783 μg g−1 for Nickel (Ni); 0.034 – 14.880 μg g−1 for Lead (Pb), and 26.230 – 98.780μg g−1 for Zinc (Zn).The mean concentration of heavy metals in the lichen and moss samples can be arranged in the decreasing order as follows: Mn > Zn > Cu > Ni > Cr > Pb > Co > Cd and the statistical analyses revealed that strong correlations exist between Cu–Pb, Cu–Zn, Pb–Ni and Mn–Zn concentrations. Some of the target heavy metals such as Cd, Cr, Mn, Ni and Zn were accumulated at higher concentrations in mosses compared to lichens from the same sampling location. There is some evidence that different site–specific characteristics affect the spatial distributions patterns and temporal trends of atmospheric deposition of heavy metals in the two oil–producing communities of Eket and Ibeno, Akwa Ibom State – Nigeria. However, a comparison with the previous study conducted in 2004by Ite et al. showed a slightly decreasing trend of atmospheric heavy metal deposition and these results confirmed that air quality has not further deteriorated in the two oil–producing communities studied over the last 10 years.

Keywords:
heavy metals lichen moss atomic absorption spectrometry Oil–producing communities

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References:

[1]  Ite, A. E., I. I. Udousoro, and U. J. Ibok, “Distribution of Some Atmospheric Heavy Metals in Lichen and Moss Samples Collected from Eket and Ibeno Local Government Areas of Akwa Ibom State, Nigeria,” American Journal of Environmental Protection, 2 (1). 22-31, 2014.
 
[2]  Ite, A. E., and U. J. Ibok, “Gas Flaring and Venting Associated with Petroleum Exploration and Production in the Nigeria's Niger Delta,” American Journal of Environmental Protection, 1 (4). 70-77, 2013.
 
[3]  Ite, A. E., U. J. Ibok, M. U. Ite, and S. W. Petters, “Petroleum Exploration and Production: Past and Present Environmental Issues in the Nigeria's Niger Delta,” American Journal of Environmental Protection, 1 (4). 78-90, 2013.
 
[4]  Eisler, R., Eisler's Encyclopedia of Environmentally Hazardous Priority Chemicals, Amsterdam, The Netherlands: Elsevier Science, 2007.
 
[5]  Etesin, U. M., A. E. Ite, T. A. Harry, C. E. Bassey, and E. W. Nsi, “Assessment of Cadmium and Lead Distribution in the Outcrop Rocks of Abakaliki Anticlinorium in the Southern Benue Trough, Nigeria,” Journal of Environment Pollution and Human Health, 3 (3). 62-69, 2015.
 
[6]  Adriano, D. C., Trace elements in terrestrial environments: biogeochemistry, bioavailability, and risks of metals, p.^pp. 867, New York; Berlin; Heidelberg: Springer, 2001.
 
[7]  Allen, S. E., Chemical analysis of ecological materials, Oxford: Blackwell Scientific Publications, 1989.
 
[8]  Markert, B. A., Plants as biomonitors : indicators for heavy metals in the terrestrial environment, Weinheim; New York: VCH, 1993.
 
[9]  Ward, N. I., “Multielement contamination of British motorway environments,” Science of the Total Environment, 93 393-401, 1990.
 
[10]  Markert, B. A., A. M. Breure, and H. G. Zechmeister, Bioindicators and biomonitors: principles, concepts and applications, Amsterdam; Boston: Elsevier, 2003.
 
[11]  Grodzińska, K., M. Frontasyeva, G. Szarek-Łukaszewska, M. Klich, A. Kucharska-Fabiś, S. F. Gundorina, and T. M. Ostrovnaya, “Trace Element Contamination in Industrial Regions of Poland Studied by Moss Monitoring,” Environmental Monitoring and Assessment, 87 (3). 255-270, 2003.
 
[12]  Shukla, V., D. K. Upreti, and R. Bajpai, “Ecosystem Monitoring,” Lichens to Biomonitor the Environment, pp. 97-170, New Delhi: Springer India, 2014.
 
[13]  Sawidis, T., G. A. Zachariadis, J. Stratis, and E. Ladukakis, “Mosses as Biological Indicators for Monitoring of Heavy Metal Pollution.,” Fresenius Environmental Bulletin, 2 193-199, 1993.
 
[14]  Sawidis, T., M. K. Chettri, G. A. Zachariadis, J. A. Stratis, and M. R. D. Seaward, “Heavy metal bioaccumulation in lichens from Macedonia in Northern Greece,” Toxicological and Environmental Chemistry, 50 (1-4). 157-166, 1995.
 
[15]  Garty, J., “Lichens as Biomonitors for Heavy Metal Pollution,” Plants as biomonitors: indicators for heavy metals in the terrestrial environment, B. A. Markert, ed., pp. 193-264, Weinheim; New York: VCH, 1993.
 
[16]  Minger, A., and U. Krähenbühl, “Moss and Lichen as Biomonitors for Heavy Metals,” International Journal of Environmental Analytical Chemistry, 67 (1-4). 41-48, 1997.
 
[17]  Rühling, Å., and G. Tyler, “An ecological approach to the lead problem,” Botaniska Notiser, 122 248-342, 1968.
 
[18]  Tyler, G., “Moss analysis – a method for surveying heavy metal deposition.”
 
[19]  Steinnes, E., “A critical evaluation of the use of naturally growing moss to monitor the deposition of atmospheric metals,” Science of the Total Environment, 160-161 243-249, 1995.
 
[20]  Abdullah, M. Z., A. B. Saat, and Z. B. Hamzah, “Assessment of the impact of petroleum and petrochemical industries to the surrounding areas in Malaysia using mosses as bioindicator supported by multivariate analysis,” Environmental Monitoring and Assessment, 184 (6). 3959-3969, 2012.
 
[21]  Markert, B., “Definitions and principles for bioindication and biomonitoring of trace metals in the environment,” Journal of Trace Elements in Medicine and Biology, 21, Supplement 1 77-82, 2007.
 
[22]  Klos, A., M. Rajfur, I. Sramek, and M. Waclawek, “Use of Lichen and Moss in Assessment of Forest Contamination with Heavy Metals in Praded and Glacensis Euroregions (Poland and Czech Republic),” Water Air and Soil Pollution, 222 (1-4). 367-376, 2011.
 
[23]  Pino, A., A. Alimonti, F. Botre, C. Minoia, B. Bocca, and M. E. Conti, “Determination of twenty-five elements in lichens by sector field inductively coupled plasma mass spectrometry and microwave-assisted acid digestion,” Rapid Communications in Mass Spectrometry: RCM, 21 (12). 1900-1906, 2007.
 
[24]  Onianwa, P. C., and S. O. Ajayi, “Heavy metal contents of epiphytic acrocarpous mosses within inhabited sites in Southwest Nigeria,” Environment International, 13 (2). 191-196, 1987.
 
[25]  Onianwa, P. C., S. O. Ajayi, O. Osibanjo, and A. Egunyomi, “Accumulation patterns of heavy metals in forest mosses from the south-west region of Nigeria,” Environmental Pollution Series B, Chemical and Physical, 11 (1). 67-78, 1986.
 
[26]  Onianwa, P. C., and A. Egunyomi, “Trace metal levels in some Nigerian mosses used as indicators of atmospheric pollution,” Environmental Pollution Series B, Chemical and Physical, 5 (1). 71-81, 1983.
 
[27]  Kakulu, S. E., “Biological monitoring of atmospheric trace metal deposition in North-Eastern Nigeria,” Environmental Monitoring and Assessment, 28 (2). 137-143, 1993.
 
[28]  Rühling, Å., Atmospheric heavy metal deposition in Europe: estimations based on moss analysis, Copenhagen: Nordic Council of Ministers, 1994.
 
[29]  Bako, S. P., S. Afolabi, and I. I. Funtua, “Spatial distribution and heavy metal content of some bryophytes and lichens in relation to air pollution in Nigeria's Guinea Savanna,” International Journal of Environment and Pollution, 33 (2). 195-206, 2008.
 
[30]  Fatoba, P. O., and T. I. Oduekun, “Assessment of metal deposition in Ilorin metropolis using mosses as bioindicators,” Nigerian Journal of Pure and Applied Science, 19 1549 - 1552, 2004.
 
[31]  Barandovski, L., M. V. Frontasyeva, T. Stafilov, R. Šajn, and T. M. Ostrovnaya, “Multi-element atmospheric deposition in Macedonia studied by the moss biomonitoring technique,” Environmental Science and Pollution Research, 22 (20). 16077-16097, 2015.
 
[32]  Loppi, S., and I. Bonini, “Lichens and mosses as biomonitors of trace elements in areas with thermal springs and fumarole activity (Mt. Amiata, Central Italy),” Chemosphere, 41 (9). 1333-1336, 2000.
 
[33]  Loppi, S., S. A. Pirintsos, and V. De Dominicis, “Soil Contribution to the Elemental Composition of Epiphytic Lichens (Tuscany, Central Italy),” Environmental Monitoring and Assessment, 58 (2). 121-131, 1999.
 
[34]  Loppi, S., E. Putortì, S. A. Pirintsos, and V. De Dominicis, “Accumulation of Heavy Metals in Epiphytic Lichens Near a Municipal Solid Waste Incinerator (Central Italy),” Environmental Monitoring and Assessment, 61 (3). 361-371, 2000.
 
[35]  Mendil, D., M. Tuzen, K. Yazici, and M. Soylak, “Heavy metals in lichens from roadsides and an industrial zone in Trabzon, Turkey,” Bulletin of Environmental Contamination and Toxicology, 74 (1). 190-194, 2005.
 
[36]  Adamo, P., S. Giordano, S. Vingiani, R. Castaldo Cobianchi, and P. Violante, “Trace element accumulation by moss and lichen exposed in bags in the city of Naples (Italy),” Environmental Pollution, 122 (1). 91-103, 2003.
 
[37]  Aksoy, A., Z. Leblebici, and M. G. Halici, “Biomonitoring of Heavy Metal Pollution Using Lichen (Pseudevernia furfuracea (L.) Zopf.) Exposed in Bags in a Semi-arid Region, Turkey,” Plant Adaptation and Phytoremediation, M. Ashraf, M. Ozturk and M. S. A. Ahmad, eds., pp. 59-70: Springer Netherlands, 2010.
 
[38]  Bajpai, R., D. K. Upreti, and S. K. Dwivedi, “Arsenic accumulation in lichens of Mandav monuments, Dhar district, Madhya Pradesh, India,” Environmental Monitoring and Assessment, 159 (1). 437-442, 2008.
 
[39]  Bajpai, R., D. K. Upreti, and S. K. Dwivedi, “Passive monitoring of atmospheric heavy metals in a historical city of central India by Lepraria lobificans Nyl,” Environmental Monitoring and Assessment, 166 (1). 477-484, 2010.
 
[40]  Bajpai, R., D. K. Upreti, S. K. Dwivedi, and S. Nayaka, “Lichen as quantitative biomonitors of atmospheric heavy metals deposition in Central India,” Journal of Atmospheric Chemistry, 63 (3). 235-246, 2009.
 
[41]  Shukla, V., D. K. Patel, D. K. Upreti, and M. Yunus, “Lichens to distinguish urban from industrial PAHs,” Environmental Chemistry Letters, 10 (2). 159-164, 2012.
 
[42]  Shukla, V., D. K. Upreti, and D. K. Patel, “Physiological attributes of lichen, Phaeophyscia hispidula in heavy metal rich sites of Dehra Dun, India,” Journal of Environmental Biology, 33 (6). 1051-1055, 2012.
 
[43]  Bargagli, R., Trace elements in terrestrial plants: an ecophysiological approach to biomonitoring and biorecovery: Springer, 1998.
 
[44]  Reimann, C., J. H. Halleraker, G. Kashulina, and I. Bogatyrev, “Comparison of plant and precipitation chemistry in catchments with different levels of pollution on the Kola Peninsula, Russia,” Science of the Total Environment, 243-244 169-191, 1999.
 
[45]  Čeburnis, D., and D. Valiulis, “Investigation of absolute metal uptake efficiency from precipitation in moss,” Science of the Total Environment, 226 (2–3). 247-253, 1999.
 
[46]  Jozwik, Z., “Heavy metals in tundra plants of Bellsund area, Spitsbergen,” Polish Polar Research, 11 401-409, 1990.
 
[47]  Riget, F., G. Asmund, and P. Aastrup, “The use of lichen (Cetraria nivalis) and moss (Rhacomitrium lanuginosum) as monitors for atmospheric deposition in Greenland,” The Science of the total environment, 245 (1-3). 137-148, 2000.
 
[48]  Jeran, Z., R. Jacimovic, F. Batic, and R. Mavsar, “Lichens as integrating air pollution monitors,” Environmental Pollution, 120 (1). 107-113, 2002.
 
[49]  Uluozlu, O. D., K. Kinalioglu, M. Tuzen, and M. Soylak, “Trace metal levels in lichen samples from roadsides in East Black Sea region, Turkey,” Biomedical Environmental Sciences, 20 (3). 203-207, 2007.
 
[50]  Nordberg, G. F., K. Nogawa, M. Nordberg, and L. T. Friberg, “Chapter 23 - Cadmium,” Handbook on the Toxicology of Metals (Third Edition), G. F. Nordberg, B. A. Fowler, M. Nordberg and L. T. Friberg, eds., pp. 445-486, Burlington: Academic Press, 2007.
 
[51]  Schroeder, W. H., M. Dobson, D. M. Kane, and N. D. Johnson, “Toxic Trace Elements Associated with Airborne Particulate Matter: A Review,” JAPCA, 37 (11). 1267-1285, 1987.
 
[52]  Pandey, V., D. K. Upreti, R. Pathak, and A. Pal, “Heavy metal accumulation in lichens from the Hetauda industrial area Narayani zone Makwanpur District, Nepal,” Environmental Monitoring and Assessment, 73 (3). 221-228, 2002.
 
[53]  Harte, J., Toxics A to Z: A Guide to Everyday Pollution Hazards, Berkeley: University of California Press, 1991.
 
[54]  Langard, S., and M. Costa, “Chapter 24 - Chromium,” Handbook on the Toxicology of Metals (Third Edition), G. F. Nordberg, B. A. Fowler, M. Nordberg and L. T. Friberg, eds., pp. 487-510, Burlington: Academic Press, 2007.
 
[55]  Hamilton, E. I., “The geobiochemistry of cobalt,” Science of the Total Environment, 150 (1-3). 7-39, 1994.
 
[56]  Kim, J. H., H. J. Gibb, P. Howe, and M. Sheffer, Cobalt and inorganic cobalt compounds: Concise International Chemical Assessment Document, No 69 9789241530699 9241530693, World Health Organization, Geneva, 2006.
 
[57]  Gál, J., A. Hursthouse, P. Tatner, F. Stewart, and R. Welton, “Cobalt and secondary poisoning in the terrestrial food chain: Data review and research gaps to support risk assessment,” Environment International, 34 (6). 821-838, 2008.
 
[58]  Frenzel, R. W., G. W. Witmer, and E. E. Starkey, “Heavy metal concentrations in a lichen of Mt. Rainier and Olympic National Parks, Washington, USA,” Bulletin of Environmental Contamination and Toxicology, 44 (1). 158-164, 1990.
 
[59]  Tüzen, M., “A Comparison of Sample Preparation Procedures for the Determination of Heavy Metals in Lichen Samples by GFAAS,” Analytical Letters, 35 (10). 1667-1676, 2002.
 
[60]  Georgopoulos, P. G., A. Roy, M. J. Yonone-Lioy, R. E. Opiekun, and P. J. Lioy, “Environmental copper: its dynamics and human exposure issues,” Journal of Toxicology and Environmental Health. Part B, Critical Reviews, 4 (4). 341-394, 2001.
 
[61]  Sweet, C. W., S. J. Vermette, and S. Landsberger, “Sources of toxic trace elements in urban air in Illinois,” Environmental Science & Technology, 27 (12). 2502-2510, 1993.
 
[62]  Romo-Kröger, C. M., and F. Llona, “A case of atmospheric contamination at the slopes of the Los Andes mountain range,” Atmospheric Environment. Part A. General Topics, 27 (3). 401-404, 1993.
 
[63]  Wiersma, G. B., M. E. Harmon, G. A. Baker, and S. E. Greene, “Elemental composition of Hylocomium splendens Hoh Rainforest Olympic National Park Washington, USA,” Chemosphere, 16 (10–12). 2631-2645, 1987.
 
[64]  WHO, Air Quality Guidelines for Europe, 2nd ed., p.^pp. 273, Copenhagen, Denmark: World Health Organization, Regional Office for Europe, 2000.
 
[65]  Pace, T. G., and N. H. Frank, Procedures for estimating probability of nonattainment of a PM10 NAAQS using total suspended particulate or inhalable particulate data, Research Triangle Park, N.C.: U.S. Environmental Protection Agency, Office of Air Quality Planning and Standards, Monitoring and Data Analysis Division, 1984.
 
[66]  Kula, E., P. Hrdlička, J. Hedbávný, and P. Švec, “Various content of manganese in selected forest tree species and plants in the undergrowth,” Beskydy, 5 (1). 19-26, 2012.
 
[67]  Zhu, F., L. Qu, W. Fan, M. Qiao, H. Hao, and X. Wang, “Assessment of heavy metals in some wild edible mushrooms collected from Yunnan Province, China,” Environmental Monitoring and Assessment, 179 (1-4). 191-199, 2011.
 
[68]  Norseth, T., and M. Piscator, “Nickel,” Handbook on the Toxicology of Metals, L. Friberg, G. Nordberg and V. B. Vouk, eds., pp. 541-553, Amsterdam; New York: Elsevier/North-Holland Biomedical Press, 1979.
 
[69]  Allen-Gil, S. M., J. Ford, B. K. Lasorsa, M. Monetti, T. Vlasova, and D. H. Landers, “Heavy metal contamination in the Taimyr Peninsula, Siberian Arctic,” Science of the Total Environment, 301 (1-3). 119-138, 2003.
 
[70]  Divrikli, U., M. Soylak, L. Elci, and M. Dogan, “Trace Heavy Metal Levels in Street Dust Samples from Yozgat City Center, Turkey,” Journal of Trace and Microprobe Techniques, 21 (2). 351-361, 2003.
 
[71]  Pacyna, J. M., and T. E. Graedel, “Atmospheric Emissions Inventories: Status and Prospects,” Annual Review of Energy and the Environment, 20 (1). 265-300, 1995.
 
[72]  Klaassen, C., Casarett & Doull's Toxicology: The Basic Science of Poisons, Seventh Edition, New York: McGraw-Hill, 2007.
 
[73]  Goyer, R. A., and T. W. Clarkson, “Toxic effects of metal,” Casarett and Doull's Toxicology : The Basic Science of Poisons, C. D. Klaassen, ed., pp. 931-979, New York: McGraw-Hill, 2007.