American Journal of Environmental Protection
ISSN (Print): 2328-7241 ISSN (Online): 2328-7233 Website: Editor-in-chief: Mohsen Saeedi, Hyo Choi
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American Journal of Environmental Protection. 2019, 7(1), 7-12
DOI: 10.12691/env-7-1-2
Open AccessArticle

The Role of Antioxidant Metabolism in Phytoremediation of Shrimp Farm Effluent by Acrostichum aureum Linn

Dipu Sukumaran1, , Justy Joseph1, Madhavan K.1 and Harikumar P.S1

1Centre for Water Resources Development and Management, Kerala, India

Pub. Date: January 15, 2019

Cite this paper:
Dipu Sukumaran, Justy Joseph, Madhavan K. and Harikumar P.S. The Role of Antioxidant Metabolism in Phytoremediation of Shrimp Farm Effluent by Acrostichum aureum Linn. American Journal of Environmental Protection. 2019; 7(1):7-12. doi: 10.12691/env-7-1-2


The study evaluates the phytoremediation capabilities of Acrostichum aureum in the treatment of shrimp farm effluent. The effluent parameters like Biochemical Oxygen Demand (BOD) (73%), Chemical Oxygen Demand (COD) (39%) and Nitrate (55%) were found reduced considerably during the treatment period of thirty days. The antioxidant concentrations of the plant were increased to cope up with the stress environment. Superoxide dismutase (SOD) in plants was increased to 70% during the treatment period. Peroxidase activity was also found to be increased from 0.02 to 0.26 mg/gFW. The increase in non-enzymatic antioxidants like chlorophyll indicated the health status of the plant during the treatment period.

phytoremediation shrimp farming antioxidant Acrostichum aureum

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[1]  Akinibile C.O., M.S. Yusoff, “Assessing water hyacinth (Eichhornia Crassipus) and lettuce (Pistia stratiotes), effectiveness in aquaculture wastewater treatment,” International Journal of Phytoremediation, 14(3). 201-211.2012.
[2]  Lananan, F., Abdul Hamid, S. H., Din, W. N. S., Ali, N. A., Khatoon, H., Jusoh, A., Endut, A, “Symbiotic bioremediation of aquaculture wastewater in reducing ammonia and phosphorus utilizing effective microorganism (EM-1) and microalgae (Chlorella sp.),” International Biodeterioration& Biodegradation, 95.127-134. 2014.
[3]  Naylor, R.I., Goldberg, R.J., Primavera, J.H., Kautsky, N., Beveridge, M.C.M., Clay, K., Folke, C., Lubchenco, J., Mooney, H. and Troell, M, “Effect of aquaculture on world fish supplies,” Nature, 405.1017-1024. 2001.
[4]  Podder, M.S., Majumder, C.B. “Sequestering of As(III) and As(V) from wastewater using a novel neem leaves/MnFe2O4 composite biosorbent,” International Journal of Phytoremediation 18. 1237-1257. 2016.
[5]  Maestri, E. and Marmiroli, N, “Transgenic plants for phytoremediation,” International Journal of Phytoremediation, 13. 264-279. 2011.
[6]  Aleksandra K., Alina W., Ewa H., and Ewa M., “Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants,” Plant Biotechnol Rep., 12(1). 1-14. 2018.
[7]  Mench, M., Schwitzguébel, J.P., Schroeder, P., Bert, V., Gawronski, S., Gupta, S., “Assessment of successful experiments and limitations of phytotechnologies: contaminant uptake, detoxification, and sequestration, and consequences to food safety,” Environ Sci Pollut Res Int., 16.876-900. 2009.
[8]  Buhmann, A. and Papenbrock, J., “Biofiltering of aquaculture effluents by halophytic plants: Basic principles, current uses and future perspectives”. Environmental and Experimental Botany, 92.122-133.2013.
[9]  Tanveer B. P., Bisma M., Inayatullah T., Qureshi M. Irfan and Reiaz Ul Rehman, “Characterization of mercury-induced stress biomarkers in Fagopyrum tataricum plants,” International Journal of Phytoremediation, 20(3). 225-236. 2017.
[10]  Jordan, F.L., Yocklic, M., Morino, K., Seaman, R., Brown, P., Glenn, E.P, “Consumptive water use and stomatal conductance of A triplex lentiform is irrigated with industrial brine in a desert irrigation district,” Agricultural and Forest Meteorology, 149. 899-912. 2009.
[11]  Brown, J.J., Glenn, E.P., Fitzsimmons, K.M., Smith, S.E, “Halophytes for the treatment of saline aquaculture effluent,” Aquaculture, 175, 225-268. 1999.
[12]  Lin, Q.X., Mendelssohn I.A, “Potential of restoration and phytoremediation with Juncus roemerianus for diesel-contaminated coastal wetlands,” Ecological Engineering, 35.85-91. 2009.
[13]  Hoang, T. T., Tu L. T., Le N. P., Dao Q. P, “A Preliminary Study on the Phytoremediation of Antibiotic Contaminated Sediment,” International Journal of Phytoremediation,15 (1). 65-76. 2012.
[14]  APHA, Examination of Water and Wastewater. 21th Edn. American Public Health Association, American Water Works Association and Water Environment Federation, Washington, DC. 2017.
[15]  Kakkar, P, Das, B, Viswanathan, P.N, “Biochemical analysis of SOD,” Indian J Biochem Biophys. 2. 130-132. 1984.
[16]  Reddy, K. P., Subhani, S.M., Khan, P.A., Kumar, K.B, “Effect of light and benzyladenine and desk treated growing leaves, Changes in the peroxidase activity,” Cell physiol. 26. 984. 1995.
[17]  Habig, W.H., Pabst, M. J., Jocoby, W. B, “Glutathione-Stransferase: The first enzymatic step in mercapturic acid formation,” J. Biol. Chem. 249. 7310-7339. 1974.
[18]  Arnon, D, “Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris,” Plant Physiology, 24. 1-15. 1949.
[19]  Van Rijn, J., Tal, Y., Schreier, “Denitrification in recirculating systems: theory and applications,” Aquacultural Engineering, 34.364-376. 2006.
[20]  Kadlec, R.H and Knight, R.L, Treatment Wetlands. CRC Press, Boca Raton (Lewis Publishers, New York. 1996.
[21]  Mahmood, Q, P. Zheng, E., Islam, R.C., “Lab scale studies on water hyacinth (Eichhornia crassipes Marts. Solms) for biotreatment of textile wastewater,” Caspian J. Environ. 3 (2). 83-88. 2005.
[22]  Kanabkaew Thongchai and Puetpaiboon Udomphon, “Aquatic plants for domestic wastewater treatment: Lotus (Nelumbo nucifera) and Hydrilla (Hydrilla verticillata),” J. Sci. Technol., 26(5), 749-756. 2004.
[23]  Kennedy, M. P., Murphy, K. J, “Indicators of nitrate in wetland surface and soilwater: interactions of vegetation and environmental factors,” Hydrology and Earth System Sciences, 8(4). 663-672. 2004.
[24]  Lin, Y.F., Jing, S.R., Lee, D.Y, “The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture,” Environmental Pollution, 123.107-113. 2003.
[25]  Bergheim, A., Kristiansen, R., Kelly, L, Treatment and utilization of sludge from land-based farms for salmon. In: Wang, J.-K. (Ed.), Techniques for Modern Aquaculture. American Society of Agricultural Engineers, Spokane, WA, USA.486-495.1993.
[26]  Metcalf and Eddy, Wastewater Engineering: Treatment, Disposal, Reuse, 3rd Edition. McGraw-Hill, New York. 1991.
[27]  Dipu, S. and Salom Gnana Thanga, V, “Heavy metal uptake, its effects on plant biochemistry of wetland (constructed) macrophytes and potential application of the used biomass”, Int. J. Environmental Engineering, 6(1). 43-54. 2014
[28]  Elstner, E. F., G. A. Wagner, W. Schutz, “Activated oxygen in green plants in relation to stress situations,” Current Topics in Plant Bioche and Phy. 7.159-189.1988.
[29]  Reddy, A.M., Kumar, S. G., G. Jyothsnakumari, S. Thimmanaik, C.Sudhakar, “Lead Induced Changes in Antioxidant Metabolism of Horse gram (Macrotyloma uniflorum (Lam.) and Bengal gram (Cicer arietinum L.),” Chemosphere. 60 (1). 97-104. 2005.
[30]  Aria, A.K., Abbaspour. H., Sar, S.S. “Antioxidant Enzymes Functions of Vetiveria zizianoides During the Absorption of Cadmium in Soil”, Electronic J Biol, 13(4).320-329. 2017.
[31]  Erinle, K.O., Jiang, Z., Li, M., “Oxidative stress response induced in an atrazine phytoremediating plant: Physiological responses of Pennisetum glaucum to high atrazine concentrations,” International Journal of Phytoremediation, 18. 1187-1194. 2016.
[32]  Gabriela A. L, Juraci Alves de Oliveira, Rafaella Teles Arantes Felipe and Fernanda Santos Farnese, “Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)”. Acta Botanica Brasilica, 31(2). 161-168. 2017.
[33]  Sormani R., E. Delannoy, S. Lageix, F. Bitton et al., “Sublethal Cadmium Intoxication In Arabidopsis thaliana Impacts Translation at Multiple Levels,” Plant and Cell Physiology, 52(2). 436-447. 2011.
[34]  Radotic, K., T. Ducic, D. Mutavdzic, “Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium,” Environmental and Experimental Botany, 44.105-113. 2000.