Chemical Engineering and Science
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Chemical Engineering and Science. 2013, 1(4), 87-90
DOI: 10.12691/ces-1-4-7
Open AccessArticle

Extraction of Citrus Flavonoids from Peel of Citrus Junos Using Supercritical Carbon Dioxide with Polar Solvent

Takuya Suetsugu1, 2, Hideo Iwai1, 2, Masahiro Tanaka3, Munehiro Hoshino3, Armando Quitain1, Mitsuru Sasaki1 and Motonobu Goto4,

1Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto, Japan

2ASCII, Co Ltd, 2349 Tabara, Kawasaki-machi, Tagawagun, Fukuoka, Japan

3Maruboshi Vinegar Co. Ltd., 2425 Tabara, Kawasaki-machi, Tagawagun, Fukuoka, Japan

4Department of Chemical Engineering, Furo-cho, Chikusa-ku, Nagoya, Japan

Pub. Date: December 15, 2013

Cite this paper:
Takuya Suetsugu, Hideo Iwai, Masahiro Tanaka, Munehiro Hoshino, Armando Quitain, Mitsuru Sasaki and Motonobu Goto. Extraction of Citrus Flavonoids from Peel of Citrus Junos Using Supercritical Carbon Dioxide with Polar Solvent. Chemical Engineering and Science. 2013; 1(4):87-90. doi: 10.12691/ces-1-4-7


Citrus juice processing residues are mainly composed of peel, juice sack and seed. The peel, especially, consists of bioactive compounds such as flavones. Supercritical carbon dioxide (SC-CO2) extraction of flavonoid was carried out at a pressure of 30 MPa and temperature ranging from 80 to 160°C. Ratio of water and ethanol as a co-solvent was varied from 0 to 100%. Flavonoids such as naringin and hesperidin have glycoside group, thus water was a good solvent for extraction of these compounds. Extraction behavior of tangeretin was quite different, the yield of tangeretin tended to increase with ethanol concentration.

flavones supercritical carbon dioxide citrus junos

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[1]  Kanno, S., Shouji, A., Asou, K. & Ishikawa, M. Journal of Pharmaceutical Sciences, 92, (2003)166-170.
[2]  Kanda, T., Akiyama, H., Yanagida, A., Tanabe, M., Goda, Y., Toyoda, M., Teshima, R., and Saito, Y., Biosci. Biotechnol. Biochem. 62(7), (1998), 1248-1289.
[3]  Masuko Kobori”, Hiroshi Shinmoto, Tojiro Tsushida, Kazuki Shinohara, Phloretin-induced apoptosis in B 16 melanoma 4 A 5 cells by inhibition of glucose transmembrane transport, Cancer Letters 119 (1997) 207-212.
[4]  Rodriguez, J., Yanez, J., Vincent, V., Alcaraz, M.,Benavente-Garcia, O., Castillo, J., Lorente, J., Lozano, J.A., Melanoma Res., 12, (2002), 99-107.
[5]  Braddock, R. J., Handbook of Citrus By-products and Processing Technology, Willy Inter Science, New York, 1999.