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Biomedical Science and Engineering

ISSN (Print): 2373-1257

ISSN (Online): 2373-1265


Content: Volume 2, Issue 1


QRS Detection and Heart Rate Variability Analysis: A Survey

1Biomedical Engineering Department, Faculty of Engineering, Jordan University of Science and Technology, Irbid, Jordan

Biomedical Science and Engineering. 2014, 2(1), 13-34
DOI: 10.12691/bse-2-1-3
Copyright © 2014 Science and Education Publishing

Cite this paper:
Rami J. Oweis, Basim O. Al-Tabbaa. QRS Detection and Heart Rate Variability Analysis: A Survey. Biomedical Science and Engineering. 2014; 2(1):13-34. doi: 10.12691/bse-2-1-3.

Correspondence to: Rami  J. Oweis, Biomedical Engineering Department, Faculty of Engineering, Jordan University of Science and Technology, Irbid, Jordan. Email:


Cardiac-related diseases have been one major cause of death for an ever increasing number of patients over the last few decades throughout the world. In response, automatic classification of cardiac rhythms using Heart Rate Variability analysis as an effective diagnostic tool has recently emerged as an important field of research. Previous researches has proved that translating and transforming HRV data into numbers can introduce highly accurate assessments of rhythm disorders. However, to obtain reliable HRV interpretation, accurate QRS detection approaches must be utilized. This work, as motivated by the arguments just presented, reviews in detail the most recent and efficient techniques related to QRS feature extraction and HRV determination all classified and presented in a convenient fashion to facilitate coverage. The study also presents a state-of-the-art updated review on QRS detection and heart rate variability analyses that could serve as a handy future reference in this field of research based on more than 200 articles reviewed in this effort.



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Different in Vitro Activation Methods for Latent Transforming Growth Factors (TGF)–β: Considerable Exogenous Factors to Promote Higher Mesenchymal-Origin Cell Proliferation in a Bioprocessing Platform

1Department of Biomedical Sciences, Faculty of Medicine, University of Leuven (KU Leuven), Leuven, Belgium

2School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Brisbane, Australia

Biomedical Science and Engineering. 2014, 2(1), 5-12
DOI: 10.12691/bse-2-1-2
Copyright © 2014 Science and Education Publishing

Cite this paper:
Partha S. Saha, Michael Doran. Different in Vitro Activation Methods for Latent Transforming Growth Factors (TGF)–β: Considerable Exogenous Factors to Promote Higher Mesenchymal-Origin Cell Proliferation in a Bioprocessing Platform. Biomedical Science and Engineering. 2014; 2(1):5-12. doi: 10.12691/bse-2-1-2.

Correspondence to: Partha  S. Saha, Department of Biomedical Sciences, Faculty of Medicine, University of Leuven (KU Leuven), Leuven, Belgium. Email:


Regenerative medicine includes two efficient techniques, namely tissue-engineering and cell-based therapy in order to repair tissue damage efficiently. Most importantly, huge numbers of autologous cells are required to deal these practices. Nevertheless, primary cells, from autologous tissue, grow very slowly while culturing in vitro; moreover, they lose their natural characteristics over prolonged culturing period. Transforming growth factors-beta (TGF-β) is a ubiquitous protein found biologically in its latent form, which prevents it from eliciting a response until conversion to its active form. In active form, TGF-β acts as a proliferative agent in many cell lines of mesenchymal origin in vitro. This article reviews on some of the important activation methods-physiochemical, enzyme-mediated, non-specific protein interaction mediated, and drug-induced- of TGF-β, which may be established as exogenous factors to be used in culturing medium to obtain extensive proliferation of primary cells.



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Biological Characterization of Crude Extract & Pure Compound Isolated from Swertia chirata Ham

1Department of Materials Science & Engineering, Rajshahi University, Rajshahi, Bangladesh

2Department of Pharmacy, Rajshahi Science & Technology University, Natore, Bangladesh

Biomedical Science and Engineering. 2014, 2(1), 1-4
DOI: 10.12691/bse-2-1-1
Copyright © 2014 Science and Education Publishing

Cite this paper:
Mst.Jesmin Sultana, Fazle Rabbi Shakil Ahmed. Biological Characterization of Crude Extract & Pure Compound Isolated from Swertia chirata Ham. Biomedical Science and Engineering. 2014; 2(1):1-4. doi: 10.12691/bse-2-1-1.

Correspondence to: Mst.Jesmin  Sultana, Department of Materials Science & Engineering, Rajshahi University, Rajshahi, Bangladesh. Email:


The fresh stem of the plant Swertia chirata Ham was extracted by rectified spirit. The crude rectified spirit extract was fractionated by using standard chromatographic techniques, on alumina gave several fractions (A, B, C, D, E & F). Fraction D, when subjected to column chromatographic analysis on neutral alumina, yielded a pure compound X-1 m.p. 180°C. X-1 was screened for its antibacterial activities against 12 pathogenic bacteria, 6 Gram positive and 6 Gram negative, by disc diffusion method at a concentration of 200 μg/disc. The results obtained were compared with those for a standard antibiotic Kanamycin. X-1 showed significant activity against Bacillus megaterium (13 mm), Bacillus subtilis (11 mm), Salmonella typhi-A (12 mm), Shigella flexeneriae (12 mm) and Klebsiella sp (13 mm) but a little activity against Staphylococcus aureus. The Minimum Inhibitory Concentrations (MIC) of X-1 determined against Bacillus megaterium and Salmonella typhi-A were 128 μg/ml and 132 μg/ml, respectively when tested in a nutrient broth medium. X-1 also showed significant activity against the brine shrimp (Artemia salina) nauplii (LC50 value of 10 μg/ml), in which the mortality rate increased with the increasing concentration of the compound, suggesting a positive correlation between brine shrimp toxicity and cytotoxicity.



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