Biomedical Science and Engineering
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Biomedical Science and Engineering. 2016, 4(1), 23-30
DOI: 10.12691/bse-4-1-3
Open AccessReview Article

Fluorescence Correlation Analysis for Diagnosis Based on Molecular Dynamics

Yasutomo Nomura1,

1Department of Systems Life Engineering, Maebashi Institute of Technology, Kamisadori, Maebashi, Japan

Pub. Date: September 22, 2016

Cite this paper:
Yasutomo Nomura. Fluorescence Correlation Analysis for Diagnosis Based on Molecular Dynamics. Biomedical Science and Engineering. 2016; 4(1):23-30. doi: 10.12691/bse-4-1-3


Fluorescence correlation spectroscopy is a powerful method in clinical laboratory where a lot of samples of patients will be determined because it enables to measure concentration and molecular weight of tested molecules without any physical separation steps. Nevertheless it may not yet be used as widely as one expected. The reason is that it is likely to be difficult for many users to understand the theoretical background. In this method, the users measured intensity fluctuation of fluorescence resulted from the molecules entering and exiting tiny volume element, namely Brownian motion in solution. Using the time series data of fluorescence intensity, the autocorrelation function was calculated. When the function was fit to the analytical model derived from diffusion theory, concentration and molecular weight of fluorophores were obtained. This minireview described the theoretical background of Brownian motion, physical meaning of the correlation analysis, and its usage properly dependent on samples from homogeneous solution to inhomogeneous cell. Furthermore the recent advances are also outlined.

fluorescence correlation spectroscopy Brownian motion diffusion theory diagnosis clinical laboratory

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