Biomedicine and Biotechnology
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Biomedicine and Biotechnology. 2018, 6(1), 15-20
DOI: 10.12691/bb-6-1-3
Open AccessArticle

Phytochemical Composition of Kalanchoe pinnata and Bidens pilosa Leaves Associated with Management of Diabetes

Kenneth Waititu1, , Caroline Jerono1, Denis Kituku1, Mary Nzuve1, Fidelis Mambo2, Paul Ngugi3 and Peter Mwethera1

1Institute of Primate Research, Kenya

2Masinde Muliro University of Science and Technology, Kenya

3Nairobi Hospital, Kenya

Pub. Date: November 21, 2018

Cite this paper:
Kenneth Waititu, Caroline Jerono, Denis Kituku, Mary Nzuve, Fidelis Mambo, Paul Ngugi and Peter Mwethera. Phytochemical Composition of Kalanchoe pinnata and Bidens pilosa Leaves Associated with Management of Diabetes. Biomedicine and Biotechnology. 2018; 6(1):15-20. doi: 10.12691/bb-6-1-3

Abstract

Background: Diabetes is responsible for rapidly increasing morbidity globally such that it has been listed among the four priority non-communicable diseases. Global prevalence of diabetes was 8.5% of the adult population by 2014 but is steadily rising. It is estimated that global prevalence of diabetes will be 472 million by 2030 with diabetic neuropathy affecting up to 236 million people. Newer interventions based on natural compounds are required since the available options are marred with diverse side effects. Plants’ natural bioactive compounds are capable of preventing development of diabetic complications via different mechanisms making them potential alternatives for its management. Kalanchoe pinnata and Bidens pilosa have been used in folkloric medicine to treat diseases including diabetes. Objective: Our study aimed at determining phytochemicals present in these two plants and their potential for use in management of diabetes. Material and Methods: Extracts from the two plants were prepared by maceration in different solvents followed by determination of presence of ten phytochemicals. Results and Discussion: Different polyphenolic compounds, glycosides and saponins were detected in aqueous extracts of both plants. Higher concentrations of flavonoids and phenolic acids were detected in aqueous extracts from B. pilosa (30.11±0.2 mg of QE/100 g and 92.7±0.1 mg of GAE/100 g) compared to K. pinnata. Conclusion: The presence of these phytochemicals qualify these two plants as candidates for development of interventions for managing type 2 diabetes.

Keywords:
diabetes complications management Kalanchoe pinnata Bidens pilosa phytochemicals

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References:

[1]  Ayah R, Joshi MD, Wanjiru R, Njau EK, Otieno CF, Njeru EK & Mutai KK. A population-based survey of prevalence of diabetes and correlates in an urban slum community in Nairobi, Kenya. BMC Public Health, 2013; 13:371.
 
[2]  WHO. Global report on diabetes. World Health Organization, 2016.
 
[3]  Levitt NS. Diabetes in Africa: epidemiology, management and healthcare challenges. Heart, 2008; 94(11): 1376-1382.
 
[4]  Christensen DL, Friis H, Mwaniki DL, Kilonzo B, Tetens I, Boit MK et al. Prevalence of glucose intolerance and associated risk factors in rural and urban populations of different ethnic groups in Kenya. Diabetes Res Clin Pract, 2009; 84(3): 303-310.
 
[5]  Pandey A, Chawla S & Guchhait P. Type-2 diabetes: Current understanding and future perspectives. IUBMB Life, 2015; 67(7): 506-513.
 
[6]  Inzucchi SE & Majumdar SK. Current Therapies for the Medical Management of Diabetes. Obstet Gynecol, 2016; 127(4): 780-794.
 
[7]  Olokoba AB, Obateru OA & Olokoba LB. Type 2 diabetes mellitus: a review of current trends. Oman Med J, 2012; 27(4): 269-273.
 
[8]  Alam U, Asghar O, Azmi S & Malik RA. General aspects of diabetes mellitus. Handb Clin Neurol, 2014; 126: 211-222.
 
[9]  Yan LJ. Redox imbalance stress in diabetes mellitus: Role of the polyol pathway. Animal Model Exp Med, 2018; 1(1): 7-13.
 
[10]  Kashihara N, Haruna Y, Kondeti VK & Kanwar YS. Oxidative stress in diabetic nephropathy. Curr Med Chem, 2010; 17(34): 4256-4269.
 
[11]  Verpoorte R, Choi YH & Kim HK. Ethnopharmacology and systems biology: a perfect holistic match. J Ethnopharmacol, 2005; 100(1-2): 53-56.
 
[12]  Saeed N, Khan MR & Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med, 2012; 12: 221.
 
[13]  Nostro A, Germanò MP, D’angelo V, Marino A & Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol, 2000; 30(5): 379-384.
 
[14]  Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochemistry & Analytical Biochemistry, 2013; 2(4).
 
[15]  Chhikara N, Devi HR, Jaglan S, Sharma P, Gupta P & Panghal A. Bioactive compounds, food applications and health benefits of Parkia speciosa (stinky beans): a review. Agric & Food Secur, 2018; 7(1): 46.
 
[16]  Krishnaiah D, Sarbatly R & Bono A. Phytochemical antioxidants for health and medicine - A move towards nature. BMBR, 2007; 1(14): 97-107.
 
[17]  Firdous SM. Phytochemicals for treatment of diabetes. EXCLI J, 2014; 13: 451-453.
 
[18]  Singh R, Kaur N, Kishore L & Gupta GK. Management of diabetic complications: a chemical constituents based approach. J Ethnopharmacol, 2013; 150(1): 51-70.
 
[19]  Sefi M, Fetoui H, Makni M & Zeghal N. Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem Toxicol, 2010; 48(7): 1986-1993.
 
[20]  Sharma B, Balomajumder C & Roy P. Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol, 2008; 46(7): 2376-2383.
 
[21]  Gulfraz M, Ahmad A, Asad MJ, Sadiq A, Afzal U, Imran M, et al, Antidiabetic activities of leaves and root extracts of Justicia adhatoda Linn against alloxan induced diabetes in rats. AJB, 2011; 10(32): 6101-6106.
 
[22]  Singh J & Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol, 2009; 123(1): 22-26.
 
[23]  Dewanjee S, Das AK, Sahu R & Gangopadhyay M. Antidiabetic activity of Diospyros peregrina fruit: effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes. Food Chem Toxicol, 2009; 47(10): 2679-2685.
 
[24]  Noh, H., & Ha, H. (). Reactive oxygen species and oxidative stress. Contrib Nephrol, 2011; 170: 102-112.
 
[25]  Choi R, Kim BH, Naowaboot J, Lee MY, Hyun MR, Cho EJ, et al. Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes. EMM, 2011; 43(12): 676-683.
 
[26]  Abdel-Raouf HS. Anatomical traits of some species of Kalanchoe (Crassulaceae) and their taxonomic value. Annals of Agricultural Sciences, 2012; 57(1): 73-79.
 
[27]  Fernandes JM, Félix-Silva J, da Cunha LM, Gomes JAS, Siqueira EMS, Gimenes LP, et al., Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom. PLOS ONE, 2016; 11(12): e0168658.
 
[28]  Cawich SO, Harnarayan P, Budhooram S, Bobb NJ, Islam S & Naraynsingh V. Wonder of Life (kalanchoe pinnata) leaves to treat diabetic foot infections in Trinidad & Tobago: a case control study. Trop Doct, 2014; 44(4): 209-213.
 
[29]  Patil SB, Dongare VR, Kulkarni CR, Joglekar MM & Arvindekar AU. Antidiabetic activity of Kalanchoe pinnata in streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action. Pharm Biol, 2013; 51(11): 1411-1418.
 
[30]  Silva FL, Fischer DCH, Tavares JF, Silva MS, de Athayde-Filho PF & Barbosa-Filho JM. Compilation of secondary metabolites from Bidens pilosa L. Molecules, 2011; 16(2): 1070-1102.
 
[31]  Bartolome AP, Villasenor IM & Yang WC. Bidens pilosa L. (Asteraceae): Botanical Properties, Traditional Uses, Phytochemistry, and Pharmacology’, Evidence-Based Complementary and Alternative Medicine, 2013. [Online].
 
[32]  Redl K, Breu W, Davis B & Bauer R. Anti-inflammatory active polyacetylenes from Bidens campylotheca, Planta Med, 1994; 60(1): 58-62.
 
[33]  Rybalchenko NP, Prykhodko VA, Nagorna SS, Volynets NN, Ostapchuk AN, Klochko VV, et al. In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. against yeasts. Fitoterapia, 2010; 81(5): 336-338.
 
[34]  Chien SC, Young PH, Hsu YJ, Chen CH, Tien YJ, Shiu SY, et al., Anti-diabetic properties of three common Bidens pilosa variants in Taiwan. Phytochemistry, 2009; 70(10): 1246-1254.
 
[35]  Liang YC, Yang MT, Lin CJ, Chang CLT & Yang WC. Bidens pilosa and its active compound inhibit adipogenesis and lipid accumulation via down-modulation of the C/EBP and PPARγ pathways. Scientific Reports, 2016; 6: 24285.
 
[36]  Ebbo AA, Mamman M, Suleiman MM, Ahmed A, and Bello A. Preliminary Phytochemical Screening of Diospyros Mespiliformis. Anat Physiol, 2014; 4(4): 1-3.
 
[37]  Samidha K, Vrushali K & Vijaya P. Estimation of Phenolic content, Flavonoid content, Antioxidant and Alpha amylase Inhibitory Activity of Marketed Polyherbal Formulation. JAPS, 2014; 4(9): 61-65.
 
[38]  Baba SA & Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. JTUSCI, 2015; 9(4): 449-454.
 
[39]  Ming-jun C, Xin Y, Yu-qing C & Chao Z. Phytochemicals for Non-insulin Diabetes Mellitus: A Minireview on Plant-Derived Compounds Hypoglycemic Activity. JFNS, 2017; 5(2): 23-27.
 
[40]  Chang CLT, Liu HY, Kuo TF, Hsu YJ, Shen MY, Pan CY & Yang WC. Antidiabetic effect and mode of action of cytopiloyne. Evid Based Complement Alternat Med, 2013; 685642.
 
[41]  Oluwole OO, & Oladunmoye MK. Phytochemical Screening and Antibacterial Activities of Bidens pilosa L. and Tridax procumbens L. on Skin Pathogens. Int J Modern Biol Med, 2017; 8(1): 24-26.
 
[42]  Bogucka-Kocka A, Zidorn C, Kasprzycka M, Szymczak G & Szewczyk K. Phenolic acid content, antioxidant and cytotoxic activities of four Kalanchoë species. Saudi J Bio Sci, 2018; 25(4): 622-630.
 
[43]  Sharker SM, Hossain MK, Haque MR, Chowdhury AA, Kaisar A, Hasan CM & Rashid MA. Chemical and biological studies of Kalanchoe pinnata (Lam.) growing in Bangladesh. APJTB, 2012; 2(3): S1317-S1322.
 
[44]  Shashank M, Khosla KK, Cathrin M & Debjit B. Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers. J Med Plants Stud, 2013; 1(2): 19-23.
 
[45]  Vinayagam R, Jayachandran M & Xu B. Antidiabetic Effects of Simple Phenolic Acids: A Comprehensive Review. Phytother Res, 2016; 30(2): 184-199.
 
[46]  Afolayan AJ & Sunmonu TO. Artemisia afra Jacq. ameliorates oxidative stress in the pancreas of streptozotocin-induced diabetic Wistar rats. Biosci Biotechnol Biochem, 2011; 75(11): 2083-2086.