Biomedicine and Biotechnology
ISSN (Print): 2378-5527 ISSN (Online): 2378-5535 Website: http://www.sciepub.com/journal/bb Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
Biomedicine and Biotechnology. 2017, 5(1), 1-5
DOI: 10.12691/bb-5-1-1
Open AccessArticle

Histopathological Effect of (Peganum harmala) Alcoholic Extract of Seeds on Liver and Kidney in Mice

Manal H.AL-Jborrey1, and Ayyad W. Al-Shahwany1

1College of science, University of Baghdad, Iraq

Pub. Date: December 08, 2017

Cite this paper:
Manal H.AL-Jborrey and Ayyad W. Al-Shahwany. Histopathological Effect of (Peganum harmala) Alcoholic Extract of Seeds on Liver and Kidney in Mice. Biomedicine and Biotechnology. 2017; 5(1):1-5. doi: 10.12691/bb-5-1-1

Abstract

Toxicity still a global problem for the environment, agriculture and ultimately human health. The purpose of the study was to examine toxicity effects of Peganum harmala herbal extract on liver and kidney of albino Swiss mice. The herbal alcoholic extract used in different doses (1000, 2000, and 3000 mg/kg b.w) for 2 weeks. The liver and kidneys dissected out and quickly fixed in 10% formal saline for histologic study. The result indicated that the treated sections of the liver showed congestion of central vein surround the hepatic lobules with sever infiltration of inflammatory cells. In addition, the Kidneys showed congestion of blood vessels with enlargement of glomerular tuft and mild-severe infiltrate of chronic inflammatory cells compared to the control. The findings indicated that the administration of Peganum harmala extract has some adverse effects on the liver and kidneys of adult mice. The study showed that alcoholic herbal extract exposure might cause harmful effects to no target organisms, including humans. Finally, further studies may be carried out to examine toxicity effects for other herbal extract to induce the safety dose dependent to histopathological changes in the liver and kidney tissues of exposed mice.

Keywords:
Peganum harmala alcoholic extract toxicity plant Histopathological change

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Si-Yuan P., Shu-Feng Z., Si-Hua G., Zhi-Ling Y., Shuo-Feng Z., Min-Ke T., Jian-Ning S., Dik-Lung M.,Yi-Fan H., Wang-Fun F. and Kam-Ming K. (2013) New Perspectives on How to Discover Drugs from Herbal Medicines: CAM’s Outstanding Contribution to Modern Therapeutics. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine; 1:1-25.
 
[2]  Raad A Kaskoos (2014) Physico-chemical Parameters, Phytochemical Screening and Antioxidant Activity of Seeds of Peganum harmala Collected from Iraq. Asian Journal of Biomedical and Pharmaceutical Sciences; 4(28): 20-24.
 
[3]  Kurata M, Suzuki M, Agar NS. (1993) Antioxidant systems and erythrocyte life-span in mammals. Biochem Physiol 106: 477-487.
 
[4]  Liu J, Tan H, Sun Y. (2009) The preventive effects of heparin superoxide dismutase on carbon tetrachloride-induced acute liver failure and hepatic fibrosis in mice. Mol Cell Biochem 327: 219-228.
 
[5]  Mahdi L.A. and Hashim J.H. (2013) Histological study of the effect of Pegenum harmala aqueous extract on liver, liver enzymes and some blood parameters in male Albino Rats. International Journal of Science and Research (IJSR); 1: 4.438.
 
[6]  Moloudizargari M, Mikaili P, Aghajanshakeri S, Asghari MH and Shayegh J. (2013) Pharmacological and therapeutic effects of Peganum harmala and its main alkaloids. Pharmacognosy Reviews; 7(14): 199-212.
 
[7]  Sabahi, M.; Mansouri, S.; Ramezanian, M. and Hoseinian, G.(1987)"Screening of plants from the southern of Iran anti-microbial activity", Inter. J. of Crude Drug Res. 25, 72-76.
 
[8]  Bancrof, J.D. and Stevens, A. (1982) the hematoxylins in: Theory and Practice of Histological Techniques, 2nd edition. London, Churchill livingstone.
 
[9]  Tradit A. J. (2011) Patient Counseling about Herbal-Drug Interactions Complement Altem Med. 8(5 Suppl): 152-163.
 
[10]  David A Y., Sonyja T., Frank L G., Zhijun L. and Jennifer C. R. (2007) Effect of an herbal extract Number Ten (NT) on body weight in rats. Chin Med. 2: 1-10.
 
[11]  Anuja P, Sunil N, Shashikant P, Vijay T, Manoj T (2012). Antidiuretic effect of polyherbal combinations in STZ induced diabetes involve inhibition of α-amylase and α-glucosidase with amelioration of lipid profile. Phytopharmacology, 2: 46-57.
 
[12]  Moura, D. J.; Richter, M. F.; Boeira, J. M.; Pêgas, H. J. A. and Saffi, J. (2007) "Antioxidant properties of beta-carboline alkaloids are related to their antimutagenic and antigenotoxic activities. Mutagenesis", 22 (4): 293-302.
 
[13]  Gonzalo J.D. (2015) Toxicosis by Plant Alkaloids in Humans and Animals in Colombia.Toxins (Basel) J. Dec; 7(12): 5408-5416.
 
[14]  Chuah L.O., Wan Yong H.W., Boon K. B. and Keong Y.S. (2013) Updates on Antiobesity Effect of Garcinia Origin (−)-HCA. Evid Based Complement Alternat Med.; 1: 1-10.
 
[15]  Mohammad A. (2012) A brief study of toxic effects of some medicinal herbs on kidney. Adv Biomed Res.; 1: 1-44.
 
[16]  El-Bahri L and Chemli R (1991). Peganum harmala L: a poisonous plant of North Africa. Vet Hum Toxico, 33: 276-77.
 
[17]  Fang Y., Wang Q.,Chao X.,Mingfeng C., Xiaoming Z.,Tingting W., Chunxiao Y., Jing F., Wenbin C., Ling G., Jiajun Z. (2014) Peroxisome Proliferator-Activated Receptor α Activation Induces Hepatic Steatosis, Suggesting an Adverse Effect. PLOS J.; 13: 12.
 
[18]  Witz M. R, Rosnx A. and Doljaums L. (1948) Observations on the Mitotic Reaction Induced in the Livers of Rats by Thiourea. United States Public Health Service, the National Cancer Institute, Bethesda, Md. Received for publication, August 6: 1-949.
 
[19]  Mac Mahon (1933) Uber die physiologische und pathologische Teilung von Kern und Zelle an Leberepithelien. Ztschr. f. mikr.-anat. Forsch., 32: 4I3-443.
 
[20]  Oyedemi SO, Adewusi EA, Aiyegoro OA, Akinpelu DA. (2011) Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic Wistar rats. Asian Pac J Trop Biomed.; 1(5): 353-358.
 
[21]  Sunil K., Smita N., Dinesh K., Gurvirender S., Sumit N. and Renu A. (2014) Evaluation of antihyperglycemic and antioxidant activities of Saraca asoca (Roxb.) De Wild leaves in streptozotocin induced diabetic mice. Asian Pacific Journal of Tropical Disease journal; (12): 60041-3.
 
[22]  Evaggelia L., Daisy V. Wilson D.V and Ye H. O (2012) Innate Immune Cells in Liver Inflammation. Mediator soft Inflammation; 2012: 1-21.
 
[23]  Robert R. M., K., Abraham N., Takanori H., Gordon F., Gundi M., Bhanu Singh B. and Jerrold M. W. (2010) Hepatic Enzyme Induction: Histopathology Toxicologic. Pathology J.; 38: 776-795.
 
[24]  Aglal A. A., Elgharbawy S.M.S., Mahmoud M.S. and Mahmoud M.R. (2015) Role of Capparis spinosa in ameliorating trichloroacetic acid induced toxicity in liver of Swiss albino mice. Life Science Journal; 12 (2):1-12.
 
[25]  Ebaid H, Dkhil MA, danfour MA, Tohamy A, Gabry MS (2007) Piroxicam induce hepatic and renal histopathological changes in mice. libyan J.med.,13; 51:56.
 
[26]  Wynn TA. (2008) Cellular and molecular mechanisms of fibrosis. J Pathol. Jan; 214(2): 199-210.
 
[27]  Mansour HH, Hafez HF, Fahmy NM (2006) Silymarin modulates Cisplatin 10 mg kg-1 induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol., 39: 656-661.
 
[28]  Nasser K., Ali M. Einia, b, Yaser S., Hoda F., Mehdi A. (2015) Effect of Peganum harmala L. on lipid metabolism and changes HMGcoA reductase in Hyper cholesterolemia induced male wistar Rat. The European Proceedings of Social & Behavioural Sciences eISSN: 2357-1330.
 
[29]  Ashraf Y N. and Hamid AM S. (2014) Aged garlic extract protects against oxidative stress and renal changes in cisplatin-treated adult male rats. Cancer Cell International 14: 92.
 
[30]  Selvamani P, Latha S, Elayaraja K, Babu PS, Gupta JK, et al. (2008) Antidiabetic activity of the Ethanol Extract of Capparis sepiaria L Leaves. India J Pharm Sci.; 70 (3): 378-380.
 
[31]  Vladimir-Knežević S, Cvijanović O, Blažeković B, Kindl M, Štefan MB, Domitrović R. (2015) Hepatoprotective effects of Micromeria croatica ethanolic extract against CCl4-induced liver injury in mice. BMC Complement Altern Med., Jul 15; 15:1-233.
 
[32]  Hayelom K., Mekbeb A., Eyasu M., Wondwossen E. and Kelbesa U. (2012) Methanolic effect of Clerodendrum myricoides root extract on blood, liver and kidney tissues of mice. African Health Sciences; 12(4): 1-10.