Biomedicine and Biotechnology
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Biomedicine and Biotechnology. 2016, 4(1), 12-18
DOI: 10.12691/bb-4-1-3
Open AccessArticle

Antibacterial and Anti-biofilm Activity of Three Phenolic Plant Extracts and Silver Nanoparticles on Staphylococcus aureus and Klebsiella pneumoniae

Ayyad W. AL Shahwany1, , Heba K. Tawfeeq2 and Shahad E. Hamed1

1Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

2Central Environmental Laboratory, College of Science, Baghdad University, Iraq

Pub. Date: December 23, 2016

Cite this paper:
Ayyad W. AL Shahwany, Heba K. Tawfeeq and Shahad E. Hamed. Antibacterial and Anti-biofilm Activity of Three Phenolic Plant Extracts and Silver Nanoparticles on Staphylococcus aureus and Klebsiella pneumoniae. Biomedicine and Biotechnology. 2016; 4(1):12-18. doi: 10.12691/bb-4-1-3

Abstract

Background: Multi-drug resistance is a growing problem in the treatment of infectious diseases and the widespread use of broad-spectrum antibiotics has produced antibiotic resistance for many human bacterial pathogens. Advances in nanotechnology have opened new horizons in Nano medicine, allowing the synthesis of nanoparticles that can be assembled into complex architectures. Method: Novel studies were carried out to assess the antibacterial effect of phenolic plants extracts and silver nanoparticles on the development of biofilm formation of bacterial species (Klebsiella pneumoniae) and (Staphylococcus aureus) using fourteen isolates from both clinical and environmental samples. Also, the experiments were conducted to study the antibacterial effect by determining the minimum inhibitory concentrations (MICs), and susceptibility test of these strains for 8 antibiotics. Moreover, the study used silver nanoparticles Ag-NPs which indicated a size range of 101.77 nm as antimicrobial agent. Although the Zingiber officinal Roscoe, Thymus vulgaris L. and Cinnamomum zeylanicum phenolic plant extracts concentrations (50, 25, 12.5, 6.25, 3.125 and 1.625 µg/ml) (W/V) were used through antibiotic and biofilm inhibition assay. Result: The result showed that the isolates behaved differently in their sensitivity to the different extracts added to their growth medium. Among the different plant phenolic extracts tested, Z. officinale showed antibiofilm efficacy against k. pneumoniae and S. aureus followed by T. vulgaris and C. zeylanicum extracts exhibited. Conclusion: The silver nanoparticles with either the plants extract compound (phenolic compound) may provide a safe and highly effective alternative to commonly used antibiotics, which are ineffective towards the antibiotic-resistant S. aureus and K. pneumonia .Also, The silver nanoparticles presented strong antimicrobial and antibiofilm activity against and K. pneumoniae and S. aureus in an indication that represents a new potential candidate for alternative antibiofilm.

Keywords:
Zingiber officinale Thymus vulgaris Cinnamomum zeylanicum antibacterial activity biofilm phenolic extracts

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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References:

[1]  Prakash P, Gnanaprakasam P, Emmanuel R, Arokiyaraj S, Saravanan M. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. For enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf B Biointerfaces. 2013. Aug 1; 108: 255-9.
 
[2]  Harshiny M, Matheswaran M, Arthanareeswaran G., Kumaran S and Rajasree S. Enhancement of antibacterial properties of silver nanoparticles-ceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis. Ecotoxicol Environ Saf. 2105. 121: 135-41.
 
[3]  Lee JH .Methicillin (Oxacillin) -Resistant Staphylococcus aureus Strains Isolated from Major Food Animals and Their Potential Transmission to Humans. Appl. Environ. Microbiol. 2003. 69 (11): 6489-6494.
 
[4]  Tsay RW, Siu LK, Fung CP, Chang FY. Characteristics of bacteremia between community-acquired and nosocomial Klebsiella pneumoniaee infection: risk factor for mortality and the impact of capsular serotypes as a herald for community- acquired infection. Arch Intern Med 2002. 162: 1021-7.
 
[5]  Dreeszen, P. H. Biofilm: The key to understanding and controlling bacterial growth in automated drinking water systems. 2nd ed. Edstrom industries Inc. 2003.
 
[6]  Reid, G., Howard, J. andGan, B. S. Can bacterial interference prevent infection? Trends Microbiol. 2001. 9: 424-428.
 
[7]  Whiteley M, Bangera MG, Bumgarner RE, Parsek MR, Teitzel GM, Lory S, Greenberg EP. Gene expression in Pseudomonas aeruginosa biofilms. Nature. 2001. 413(6858): 860-4.
 
[8]  Allaker RP, Memarzadeh K. Nanoparticles and the control of oral infections. Int J Antimicrob Agents. 2014. 43:95-104.
 
[9]  Sondi I, Salopek-Sondi B Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci. 2004. 275:177-182.
 
[10]  Hamed, SE, Al Shahwany AW. Increasing antimicrobial activity of some plant extracts against antibiotic resistant Staphylococcus aureus by using silver nanoparticles. World J Exp Biosci. 2016. Vol. 4, Number 1.
 
[11]  Cox PA. Balick MJ. The ethnobotanical approach to drug discovery. Sci Am. 1994. 270(6): 82-7.
 
[12]  Harborne JB. Phytochemical methods. Chapman and Hall. New York 2nd Ed 1984: 288.
 
[13]  Bardawell SK .D-amino acids: Prospects for new therapeutic agent. Journal of medical and bioengineering. 2014. 3(3): p197.
 
[14]  Morello, JA, Mizer HE., Granato PA. Laboratory Manual and Workbook in Microbiology Applications to Patient Care. 18th .ed. The McGraw-Hill Companies, Inc., New York. 2006. P.95-99.
 
[15]  Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann. MC. Toxicological Sciences. 2005. 88(2): 412-419.
 
[16]  Andrews, J. M. (2001). Determination of minimum inhibitory concentrations, Journal of Antimicrobial Chemotherapy, Vol. 48, no. 1, p: 5-16.
 
[17]  McBain, A. J., Ledder, R. G., Srinivasan, P. and Gilbert, P. (2004). Selection for high-level resistance by chronic triclosan exposure is not universal, Journal of Antimicrobial Chemotherapy, 53 (5), p: 772-777.
 
[18]  Amaral, M. M., Coelho, L. R., Flores, R. P., Souza, R. R., Silva-carvalho, M. C., Teixeira, L. A., Ferreira-carvalho, B. T. and Figueiredo, A. M. S. (2005). The predominant variant of the Brazilian epidemic clonal complex of methicillin-resistant S. aureus has an enhanced ability to produce biofilm and adhere to invade airway epithelial cells. J Infect. Dis. 192:801-810.
 
[19]  Mason, R. L., Gunst, R. F. and Hess, J.L. (2003). Statistical design and analysis of experiments. Welly-Interscience, New Jersey.
 
[20]  CLSI, Clinical and Laboratory Standards Institute. 2013. http://clsi.org/membership/current/my-clsi/.
 
[21]  Cetti RJ, Venn S, Woodhouse CR. The risks of long-term nitrofurantoin prophylaxis in patients with recurrent urinary tract infection: A recent medico-legal case. BJU Int. 2009. 103: 567-569.
 
[22]  Seguin, JC, Walker RD, Caron JP, Kloos WE, George CG, Hollis R.J, Jones RN, Pfaller MA. Methicillin-Resistant Staphylococcus aureus Outbreak in a Veterinary Teaching Hospital: Potential Human-to-Animal Transmission. J. Clin. Microbiol. 1999. 37(5): 1459-1463.
 
[23]  Lee KH, Hui KP, Tan WC, Lim TK. Klebsiella bacteraemia: a report of 101 cases from National University Hospital, Singapore. J Hosp Infe ct.1994. 27:299-305.
 
[24]  Gao D, Zhang Y. Comparative antibacterial activities of crude polysaccharides and flavonoids from Zingiber officinale and their extraction. Asian Journal of Traditional Medicines. 2010. (1). 235-238.
 
[25]  Michael d. Common spices protect bacteria during irradiation 1999. Am. Chem. Soc.1999. 2: 270-275.
 
[26]  Melvin M.J, Jayachitra J, Vijayapriya M. Antimicrobial activity of some common spices against certain human pathogens. Journal of Medicinal Plants Research. 2009. 3(11): 1134-1136.
 
[27]  Beenken KE, Mark LN, Griffin LM, Zielinska AK, Show LN, Rice KC, Horswill AR, BaylesK W and Smeltzer MS. Epistatic relationships between sar A and agr in Staphylococcus aureus biofilm formation. Plos one. 2010. 5: p 10790.
 
[28]  Lazar, V. Quorum sensing in biofilms—How to destroy the bacterial citadels or their cohesion/power?Anaerobe, .2011. 17, 280-285.
 
[29]  Ali BH, Blunden G, Tanira MO and Nemmar A. Some hytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): Areview of recent research. Food Chem. Toxicol. 2008. 46, 409-420.
 
[30]  Stoilova I, Krastanov A, Stoyanova A, Denev P, Gargova S. Antioxidant activity of a ginger extract (zingiber officinale). Food chem.2007. 102: 764-770.
 
[31]  Yahya M., Saifuddin N , Hamid A. Zingiber officinale ethanolic extract inhibits formation of pseudomonas aeruginosa biofilm . Int J Pharm Bio Sci, .2013. Volume 3: Issue 1, page 46-54.
 
[32]  Alves-Silva MJ, Dias dos Santos MS, Pintado EM, Pérez-Álvarez AJ, and Juana FernándezLópez J, Viuda-Martos M. Chemical composition and in vitro antimicrobial, antifungal and antioxidant properties of essential oils obtained from some herbs widely used in Portugal. Food Control. 2013. 32: 371-378.
 
[33]  Nadia Z, Rachid M. Antioxidant and antibacterial activities of Thymus vulgaris L. Medicinal. Aromatic Plant Res J .2013. 1: 5-11.
 
[34]  Aghsaghali AS, Syadati SA, Fathi H. Some of thyme (Thymus vulgaris) properties in ruminant’s nutrition. Ann Biol Res .2012. 3: 1191-1195.
 
[35]  Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol.2004. 94: 223-253.