American Journal of Sports Science and Medicine
ISSN (Print): 2333-4592 ISSN (Online): 2333-4606 Website: Editor-in-chief: Ratko Pavlović
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American Journal of Sports Science and Medicine. 2016, 4(2), 26-32
DOI: 10.12691/ajssm-4-2-1
Open AccessArticle

Progressive Resistance Training Modulates the Expression of ACTN2 and ACTN3 Genes and Proteins in the Skeletal Muscles

Neda khaledi1, , Rana Fayazmilani2, Abbas Ali Gaeini3 and Arash Javeri4

1Exercise Physiology, Kharazmi University

2Exercise Physiology, Shahid Beheshti University

3Exercise Physiology, The University of Tehran

4Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Pub. Date: April 25, 2016

Cite this paper:
Neda khaledi, Rana Fayazmilani, Abbas Ali Gaeini and Arash Javeri. Progressive Resistance Training Modulates the Expression of ACTN2 and ACTN3 Genes and Proteins in the Skeletal Muscles. American Journal of Sports Science and Medicine. 2016; 4(2):26-32. doi: 10.12691/ajssm-4-2-1


Purpose: Mammalian skeletal muscle has the two isoforms of actin binding protein, α-Actinin-2 and α-Actinin-3, which are located in the skeletal muscle Z-line where they cross-link the actin thin filaments. There is a common stop codon polymorphism R577X in the ACTN3 gene. Several association studies have demonstrated that the ACTN3 R577X genotype influences athletic performance. The response of α-Actinins to resistance exercise training is little understood. Methods: Female Sprague-Dawley rats were assigned to control (C; n = 10) and resistance training (T; n = 12) groups. Training consisted of climbing a ladder carrying a load suspended from the tail. After training, fast (Flexor halluces longus, FHL) and slow (Soleus) hind limb muscles from each group was examine to study the effect of resistance training on muscle mass. Gene expression and protein levels of both Actn3, Actn2 were examined. Results: The resistance trained group had a significantly greater absolute muscle mass in FHL (P=0.011). We also found that Actn3 and Actn2 gene expression levels increased significantly in FHL and Soleus muscles by mean factors of 2.16, and 2.91, respectively. α-Actinin-2 protein expression increased significantly in training group (P=0.025) while, α-actinin-3 protein expression remained similar in training & control groups (P=0.130). The most important finding of this study showed that both α-actinin-3 and α-actinin-2 mRNA levels were up-regulated after 8wk of resistance training (P≤0.05). Conclusion: Our results provide a new insight into the impact of progressive resistance training and evaluating the role of α-actinins responsiveness.

vertical ladder α–actinins sarcomere Z-line resistance training

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