American Journal of Pharmacological Sciences
ISSN (Print): 2327-6711 ISSN (Online): 2327-672X Website: http://www.sciepub.com/journal/ajps Editor-in-chief: Srinivas NAMMI
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American Journal of Pharmacological Sciences. 2015, 3(6), 126-131
DOI: 10.12691/ajps-3-6-1
Open AccessArticle

Antibacterial and Antioxidant Activities of Hydro-Ehanol Extracts of Barks, Leaves and Stems of Annona muricata

S. H. Riwom Essama1, , M. A. Nyegue1, C. Ndoye Foe1, 2, K. Kamga Silihe1, S. P. Bouopda Tamo1 and F. X. Etoa1

1Laboratory of Microbiology, Department of Microbiology, University of Yaoundé I, P. O. Box 812 Yaoundé, Cameroon

2Laboratory of Phytobiochemistry, Department of Biochemistry, University of Yaoundé I, P. O. Box 812 Yaoundé, Cameroon

Pub. Date: January 05, 2016

Cite this paper:
S. H. Riwom Essama, M. A. Nyegue, C. Ndoye Foe, K. Kamga Silihe, S. P. Bouopda Tamo and F. X. Etoa. Antibacterial and Antioxidant Activities of Hydro-Ehanol Extracts of Barks, Leaves and Stems of Annona muricata. American Journal of Pharmacological Sciences. 2015; 3(6):126-131. doi: 10.12691/ajps-3-6-1

Abstract

The purpose of this study was to evaluate the in-vitro antibacterial activity of hydro-ethanol extracts of stems, barks and leaves of Annona muricata against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabillis and Bacillus cereus, followed by the evaluation of their antioxidant activities. The sensitivity of the bacteria to extracts was evaluated by the well diffusion method and the inhibition parameters of the bacterial growth were determined by the micro-dilution assay. The Minimum Bactericidal concentrations (MBC) obtained were between 6.25 and 25.00 mg/ml. Pseudomonas aeruginosa was the most sensitive strain with a MBC value of 6.25 mg/ml obtained from leaves extract. The antioxidant activity was evaluated using1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing antioxidant power (FRAP) assay. Hydro-ethanol extract of barks exhibited the highest DPPH scavenging activity of 0.010 ± 0.004 g/mg and the highest reducing power with ascorbic acid equivalence of 49.45 ± 0.067 mg EAA/gw. This extract has presented only the highest phenolic content 25.00 ± 1.070 mg EAA/gw. The phytochemical screening revealed that the plant contain bioactive compounds such as steroids, triterpenes and alkaloids. These results confirm the use of this plant in traditional medicine for the treatment of bacterial infections and diseases associated to oxidative stress such as diabetes.

Keywords:
Annona muricata Antibacterial susceptibility Antioxidant activity Bioactive compounds

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References:

[1]  OMS, “Model Prescribing Information-Drugs used in bacterial infections”. Genève: OMS, (2010): 2.
 
[2]  OMS, “Rapport sur la Sante du Monde”. (2009).
 
[3]  Iserin, P., “Identification, Préparations, soins.” Larousse encyclopédie des plantes médicinales, Dorling Kindersiey Limited, Londres, (2001).
 
[4]  Maurice, N., “L'herboristerie d'antan à la phytothérapie moléculaire du XXIe siècle. Ed.Lavoisier, (1997): 12-14.
 
[5]  OMS, “Rapport sur la Sante du Monde. (2002).
 
[6]  Diallo, A., “Etude des plantes médicinales de niafunke (région Tombouctou) Photochimie et pharmacologie de Maerua crassifolia Forsk. (Capparidacée).” Thèse de Doctorat. Université de Bamako, (2005): 125.
 
[7]  Heinrich, M., “Parasitological and microbiological evaluation of Mixe Indian medicinal plants (Mexico).” Journal of Ethnopharmacology, 36(1), (1995): 81-85.
 
[8]  Guadano, A., “Insecticidal and mutagenic evaluation of two annonaceous acetogenins.” Journal of Natural Products, 63(6), (2000):773-6.
 
[9]  Leaman, D., “Malaria remedies of the Kenyah of the ApoKayan, East Kalimantan, and Indonesian Borneo: A quantitative assessment of local consensus as an indicator of biological efficacy.” Journal of Ethnopharmacology, 49(1), (1995):1-16.
 
[10]  Lopez Abraham, A., “Plant ex tracts with cytostatic properties growing in Cuba.” I. Rev. Cubana de Medecina tropical, 31(2), (1979): 97-104.
 
[11]  Rodríguez, H., “Plantas contra plagas; potencial practico de ajo, anona, nim, chile y tabaco. Editado por la red de acción sobre plaguicidas y alternativa en México.” Texcoco, Estado de México, (2000): 133.
 
[12]  Harbone J. (1998). Phytochemical Methods. A Guide to Modern Techniques of Plants Analysis; Third edition ISSN: 0-412-5720-5 (HB) and 0-412-5727-0 (PB).
 
[13]  Sofowora E. (1993). Phytochemical screening: Medicinal Plants and Traditional Medicine in Africa, Spectrum Books Ltd, Ibadan, Nigeria: pp270-289.
 
[14]  Hernandez, N., Tereschuk M. and Abdala, L., “Antimicrobial activity of flavonoids in medicinal plants from Tafı´ del Valle.” J. Ethnopharmacology, 73, (2000): 317-322.
 
[15]  Singleton, C. and Rossi, J., “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents.” Journal of Enology and Viticulture, 16, (1965): 144-158.
 
[16]  Clinical and Laboratory Standards Institute (CLSI), “Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts; approved guideline, M44-A.” 24, (2005): 12-28.
 
[17]  National Committee for Clinical Laboratory Standards Methods for Dilution Antimicrobial Susceptibility (NCCLS), “Tests for Bacteria That Grow Aerobically; approved standard M27-A9, Wayne.” Clinical and Laboratory Standards Institute, Wayne. Clinique, 32 (9), (2012): 12-28.
 
[18]  Brand-Williams, W. and Cuvelier, M., Berset, C., “Use of a free radical method to evaluate antioxidant activity.” Food Sciences Technology, 28, (1995): 25-30.
 
[19]  Benzie, and Strain, “The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay.” Analytical Biochemistry, 239, (1996): 70-76.
 
[20]  Fauchère J. and Avril J. (2002). Bactériologie Générale et Médicale. Ellipses éditent, Paris. 365.
 
[21]  Solomon-wisdom, G., ugoh S. and Mohammed B., “Phytochemical screening and antimicrobial activities of Annona muricata leaf extract.” American Journal of Biological, Chemical and Pharmaceutical Sciences, 2 (1), (2014): 01-07.
 
[22]  Takeo, O., Masato, K., Keiko S., Rika, O., Junko, M., Hiroshi, I., Hiroyuki, K., Toshi, A. and Tamer, F., “Free radicals, Types, sources and damaging reactions, Internal Medicine Articles. (http://www.doctorslounge.com/primary/articles/; 12/11/2013) (2003).
 
[23]  Vijayameena, C., Subhashini, G., Loganayagi, M. and Ramesh, B., “Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata.” Int. J. Curr. Microbiol. App. Sci, 2(1), (2013): 1-8.
 
[24]  Cowan, G., “Plant products as antimicrobial agents.” Clinical Microbiology Review, 12, (1999): 564-582.
 
[25]  Kansole, M., “Etude ethnobotanique, phytochimique et activités biologiques de quelques lamiacées du Burkina Faso.” Diplôme d’études approfondies en sciences biologiques appliquées, université de Ouagadougou, (2009): 3.
 
[26]  Boris, R., “Natural products research: perspectives from a major pharmaceutical company.” Journal of Ethnopharmacology, 51, (1996): 29-38.
 
[27]  Phillipson, and O,Neill, New leads to the treatment of protozoal infections based on natural product molecules.” Acta Pharmacology Nord, 1, (1997): 131-144.
 
[28]  Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P. and Vidal, N., “Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds.” Food Chemistry, 97, (2006): 654-660.
 
[29]  Hayouni, E., Abedrabba, M., Bouix, M. and Hamdi, M., “The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts.” Food Chemistry, 105, (2007): 1126-1134.
 
[30]  Turkmen, N., Velioglu, Y., Sari, F. and Polat G., “Effect of extraction conditions on measured total polyphenol contents and antioxidant and antibacterial activities of Black Tea.” Molecules, 12, (2007): 484-496.
 
[31]  Tawaha, K., Alali, F., Gharaibeh, M., Mohammad, M. and El-Elimat, T., “Antioxidant activity and total phenolic content of selected Jordanian plant species.” Food Chemistry, 104, (2007): 1372-1378.
 
[32]  Nabila, B., Fawzia, A. and Tatjana, K., “Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus.” Comptes rendus Chimie, 12, (2009): 1259-1266.
 
[33]  Su, J. D., Osawa, T., Kawakishi, S. and Nail, M., “Tannin antioxidants from Osbeckia chinensis. Phytochemistry, 27, (1988): 1315-1319.
 
[34]  Muanda, F., “Identification de polyphénols, évaluation de leur activité antioxydante et étude de leurs propriétés biologiques.” Thèse soutenue en vue de l’obtention du grade de Docteur de l’Université Paul Verlaine-Metz, (2010).