American Journal of Pharmacological Sciences
ISSN (Print): 2327-6711 ISSN (Online): 2327-672X Website: Editor-in-chief: Srinivas NAMMI
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American Journal of Pharmacological Sciences. 2014, 2(5A), 8-14
DOI: 10.12691/ajps-2-5A-2
Open AccessArticle

Optimization and Permeation Study of Novel Topically Applied Antilipemic Lotion Using Central Composite Design

Asma Aslam1, Rummana Riaz1, Yasser Shahzad2, Atif Akber3, Ikram Ullah Khan4, and Syed Nisar Hussain Shah5

1Multan College of Pharmacy and Management Sciences, Pakistan

2Centre of Excellence for Pharmaceutical Sciences, North-West University, South Africa

3Department of Statistics, Bahauddin Zakariya University, Multan, Pakistan

4College of Pharmacy, Government College University, Faisalabad, Pakistan

5Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan

Pub. Date: September 30, 2014

Cite this paper:
Asma Aslam, Rummana Riaz, Yasser Shahzad, Atif Akber, Ikram Ullah Khan and Syed Nisar Hussain Shah. Optimization and Permeation Study of Novel Topically Applied Antilipemic Lotion Using Central Composite Design. American Journal of Pharmacological Sciences. 2014; 2(5A):8-14. doi: 10.12691/ajps-2-5A-2


In this study, synergistic effect of penetration enhancers, namely propylene glycol (PG) and sodium lauryl Sulphate (SLS) on transdermal absorption of 2-Methyl-2-phenoxy-propionic acid (MPA) was evaluated with aid of response surface methodology (RSM) based formulations. The prepared formulations were characterised and subsequently subjected to diffusion cell experiments through a well-established skin mimic, namely silicone membrane. Data obtained were statistically analysed using analysis of variance (ANOVA) and regression analysis. Comparisons were made with the control and among different parameters of formulations. PG (X1) and SLS (X2) were selected as independent variables, whereas the cumulative amount of MPA were used to calculate dependent permeation kinetic parameters like lag time (tlag) and enhancement ratios (ER). It was observed from the results that PG had a significant effect on MPA absorption from the lotion followed by SLS and their combination showed a significant enhancement (p< 0.05) in the flux from the formulated lotions compared to the control. The highest enhancement ratio (ER=8.6) was observed for L4. Contour plots were then drawn to depict the relationship between independent and response variables. Among the formulations, L4 showed highest flux value with less lag time (12.2 min), thereby, identified and selected as an optimized formulation. In conclusion, the combination of PG and SLS can be successfully utilized as permeation enhancers for transdermal drug delivery of MPA with minimal side effects.

propylene glycol sodium lauryl sulphate permeation response surface methodology lotion

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[1]  Sierra M.L., Beneton V., Boullay A-B., Boyer T., Brewster A.G., Donche F. Substituted 2-[(4-aminomethyl) phenoxy]-2-methylpropionic acid PPARĪ± agonists. 1. Discovery of a novel series of potent HDLc raising agents. Journal of medicinal chemistry, 50, 685-695, 2007.
[2]  Kennard C.H.L., Smith G., White A. Structural aspects of phenoxyalkanoic acids. The structures of phenoxyacetic acid,(+-)-2-phenoxypropionic acid,(+-)-2-(4-chlorophenoxy) propionic acid, 2-methyl-2-phenoxypropionic acid and 2-(4-chlorophenoxy)-2-methylpropionic acid. Acta Crystallographica Section B: Structural Crystallography and Crystal Chemistry, 38, 868-875, 1982.
[3]  Thompson J.P., Christopher M.M., Ellison G.W., Homer B.L., Buchanan B.A. Paraneoplastic leukocytosis associated with a rectal adenomatous polyp in a dog. Journal of the American Veterinary Medical Association, 201, 737-738, 1992.
[4]  Mokale S.N., Elgire R., Sakle N., Shinde D.B. Synthesis, hypolipidemic and hypoglycemic activity of some novel 2-(4-(2-substituted aminothiazole-4-yl) phenoxy)-2-methyl propanoic acid derivatives. Bioorganic & medicinal chemistry letters, 21, 682-685, 2011.
[5]  Prausnitz M.R., Langer R. Transdermal drug delivery. Nature Biotechnology, 26, 1261-1268, 2008.
[6]  Guy R.H., Hadgraft J. Transdermal drug delivery: A simplified pharmacokinetic approach. International Journal of Pharmaceutics, 24, 267-274, 1985.
[7]  Alexander A., Dwivedi S., Ajazuddin., Giri T.K., Saraf S., Saraf S. Approaches for breaking the barriers of drug permeation through transdermal drug delivery. Journal of Controlled Release, 164, 26-40, 2012.
[8]  Cooperberg B.A., Cryer P.E. Insulin reciprocally regulates glucagon secretion in humans. Diabetes, 59, 2936-2940, 2010.
[9]  Fox L.T., Gerber M., Du Plessis J., Hamman J.H. Transdermal drug delivery enhancement by compounds of natural origin. Molecules, 16, 10507-10540, 2011.
[10]  Shahzad Y., Khan Q., Hussain T., Shah S.N.H. - Influence of cellulose derivative and ethylene glycol on optimization of lornoxicam transdermal formulation. International Journal of Biological Macromolecules, 61, 26-32, 2013.
[11]  Shah S.N.H., Tahir M.A., Safdar A., Riaz R., Shahzad Y., Rabbani M. Effect of permeation enhancers on the release behavior and permeation kinetics of novel tramadol lotions. Tropical Journal of Pharmaceutical Research, 12, 27-32, 2013.
[12]  Williams A.C., Barry B.W. Penetration enhancers. Advanced Drug Delivery Reviews, 56, 603-618, 2004.
[13]  Som I., Bhatia K., Yasir M. Status of surfactants as penetration enhancers in transdermal drug delivery. Journal of Pharmacy and Bioallied Sciences, 4, 2-9, 2012.
[14]  Shahzad Y., Afreen U., Shah S.N.H., Hussain T. Applying response surface methodology to optimize nimesulide permeation from topical formulation. Pharmaceutical Development and Technology, 18, 1391-1398, 2013.
[15]  Javadzadeh Y., Shokri J., Hallaj-Nezhadi S., Hamishehkar H., Nokhodchi A. Enhancement of percutaneous absorption of Finasteride by cosolvents, cosurfactant and surfactants. Pharmaceutical Development and Technology, 15, 619-625, 2010.
[16]  Zhu X., Ji J., Huang D., Zhu Y., Tang C., Yang X. Discovery, Synthesis and Evaluation of Novel Cholesterol Absorption Inhibitors. Chemical Biology & Drug Design, 80, 426-433, 2012.
[17]  Shah S.N.H., Hussain T., Khan I.U., Asghar S., Shahzad Y. Formulation study of topically applied lotion: in vitro and in vivo evaluation. Bioimpacts, 3, 11-99, 2013.
[18]  Chang J.S., Wu P.C., Huang Y.B., Tsai Y.H. In-vitro evaluation of meloxicam permeation using response surface methodology. Journal of Food and Drug Analysis, 14, 236-241, 2006.
[19]  Megrab N.A., Williams A., Barry B. Oestradiol permeation through human skin and silastic membrane: effects of propylene glycol and supersaturation. Journal of Controlled Release, 36, 277-294, 1995.
[20]  Marrakchi S., Maibach H.I. Sodium lauryl sulfate-induced irritation in the human face: Regional and age-related differences. Skin Pharmacology and Physiology, 19, 177-180, 2006.
[21]  Molgaard B., Hoelgaard A. Vehicle effect on topical drug delivery. I. Influence of glycols and drug concentration on skin transport. Acta Pharmaceutica Suecica, 20, 433-442, 1983.
[22]  Barry B.W. Mode of action of penetration enhancers in human skin. Journal of Controlled Release, 6, 85-97, 1987.
[23]  Kaushik D., Costache A., Michniak-Kohn B. Percutaneous penetration modifiers and formulation effects. International Journal of Pharmaceutics, 386, 42-51, 2010.
[24]  Waters L.J., Dennis L., Bibi A., Mitchell J.C. Surfactant and temperature effects on paraben transport through silicone membranes. Colloids and Surfaces B: Biointerfaces.108, 23-28, 2013.