American Journal of Public Health Research
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American Journal of Public Health Research. 2019, 7(6), 217-221
DOI: 10.12691/ajphr-7-6-5
Open AccessArticle

Effect of Curcumin Extracts on the Islets of Langerhans Cells of Diabetic White Rats Induced by Alloxan: A Histological Study

Morsy A. Aboelgoud1, , Ahmad M. Alessi2 and Ahmad M.Ihab3

1Anatomy Department, Faculty of Medicine, Tanta University, Egypt

2Chemistry Department Al-Qunfudhah University College Umm Al-Qura University, KSA

3Public Health Department, Faculty of Health Sciences Umm Al-Qura University, KSA

Pub. Date: December 29, 2019

Cite this paper:
Morsy A. Aboelgoud, Ahmad M. Alessi and Ahmad M.Ihab. Effect of Curcumin Extracts on the Islets of Langerhans Cells of Diabetic White Rats Induced by Alloxan: A Histological Study. American Journal of Public Health Research. 2019; 7(6):217-221. doi: 10.12691/ajphr-7-6-5

Abstract

Background: Diabetes mellitus is an unpredictable issue that is portrayed by hyperglycemia coming about because of breakdown in insulin discharge and activity. As of late curcumin extract have been reported to be useful in treatment of type II diabetes. Aim of the work: In this research curcumin extract was used instead of chemical drugs for the treatment of diabetes mellitus. Material and methods: 30 male adult albino rats aged 5-7 months and their average weight was 150-200 grams were divided into three groups. The first one (n=10) control group, the second group (n=10) was injected with a single dose of alloxan (42 mg/kg). The third group (n=10) was injected with alloxan and after 3 days was received curcumin extract orally 80mg/kgm daily for one month. The rats were scarified after the last dose of the drug and their pancreases were removed, fixed and different sections were prepared and stained for light microscope study. Results: After induction of diabetes the islets of Langerhans revealed architectural disarray. The islets cells showed atrophy in size with cellular necrobiosis associated with cystic dilatation and luminal eosinophilic casts. The intensity anti-insulin antibody reaction was decreased. So donation of curcumin extract reduced the islets cells damage and increase in the intensity of anti-insulin antibody reaction. Conclusion: The present study revealed that curcumin extract has potential effect in treatment of type II diabetes.

Keywords:
pancreas islets of Langerhans diabetes mellitus Alloxan curcumin extract white rats

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References:

[1]  Slack, J. M. W. I995. Developmental biology of the pancreas. Development; 121(6): I569-I580.
 
[2]  Kumar, G. P., Kumar, S. D. and Subremanian, P. S. 2006.Anti-diabetic activity of fruits of Terminalia Chebulaonstreptozotocin induced diabetic rats. J Health Sci. 52: 283-291.
 
[3]  Inouye, M., Mio, T. and Sumino, K. I999. Link between glycogen and lipo- oxidation in red blood cells in diabetes. Clin Chem. Acta; 285: 35-44.
 
[4]  Bonnefont- Rousselot, D., Bastard, J. P. and Delatter, J. 2002. Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes Metab; 6: 163-76.
 
[5]  Gumieniczek, A., Hopkata, H., Zbigniew, W. and Justyna, N. 2002. Changes in antioxidant status of heart muscle tissue in experimental diabetes in rabbits. Acta Biochemica Polonica; 49(2) 529-535.
 
[6]  Jin, Xue, Jin, L.J., Li, S.Y. and Xu, Y.P. 2008. Antioxidant and pancreas protective effect of aucubin on rats with streptozotocin-induced.Eur J Pharmacol; 582: I62-I67.
 
[7]  Jakus, V. 2000. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. BratistLekListy; 101 (10): 541-551.
 
[8]  Somlak, C., Suthee R., Rataya L., Chada P, and Siwanon J. 2012. Curcumin Extract for Prevention of Type 2 Diabetes. Diabetes Care; 35(II): 2121-2127.
 
[9]  Conteas, C.N., Panossian, A.M., Tran, T.T., and Singh, H.M. 2008. Treatment of HIV associated diarrhea with curcumin. Digestive disease and sciences. 45(10): 2188-2191.
 
[10]  Gota, V.S., Maru, G.B., Soni, T.G., et al. 2010. Safety and pharmacokinetics of a solid lipid curcumin particle formulation in osteosarcoma patients and healthy volunteers. Journal of Agricultural and Food Chemistry. 58(4): 2095-2099.
 
[11]  Harish, G, Venkateshappa, C., Mythri, R.B., et al. 2010. Bioconjugates of Curcumin display improved protection against glutathione depletion mediated oxidative stress in a dopaminergic neuronal cell line: implications for Parkinson’sdisease. Bio-organic and Medicinal Chemistry. 18(7): 2631-2638.
 
[12]  Amanda, N.L., Lucas, L. C., and César T. S., 2015. Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans.J Diabetes Res. 20I5; 20I5: 494578.
 
[13]  Shabeer, J., Srivastava, R.S., Singh, S.K., 2009. Antidiabetic and antioxidant effect of various fractions of Phyllanthus simplex in alloxan diabetic rats. J. Ethnopharmacology. I24: 34-38.
 
[14]  Arun, N, and Nalini, N. 2002. Efficacy of turmeric on blood sugar and polyol pathway in diabetic albino rats. Plant Foods for Human Nutrition. 57(1): 41-52.
 
[15]  Banchroft, J.D.; Stevens, A. and Turner, D.R. 1996. Theory and practice of histological techniques.4th ed. Churchil Livingstone, New York, London, San Francisco, Tokyo.
 
[16]  Kanter, M., Uysal, T. and Kara, O. 2006. Depression of glucose levelsand partial restoration of pancreatic B-cell damage by melatonin in streptozotozin-induced diabetic rats. Arch. Toxicol; 80: 362-369.
 
[17]  Patel, D., Gidwani, B., Sarwa, K., Kaur, C.D., Dhongade, H.J., Sahu, P., Sahu, V., 2016. A comprehensive review on the anti-diabetic activity of Momordicacharantia and Syzygiumcumini seeds. Int. J. Biol. Pharm. Allied Sci. 5: 1561-1575.
 
[18]  Anfenan, M. L. K., 2014. Evaluation of nutritional and antidiabetic activity of different forms of Ginger in rats. Middle-East J Scientific Res. 2I: 56-62.
 
[19]  Jorns, A., Mundy, R., Tiedge, M. and Lenzen, S. 1997. Comparabbitive toxicity of alloxan, N-alkylalloxans and ninhydrin to isolated pancreatic islets in vitro. J. of Endocrinology; 155: 283-293.
 
[20]  Wolf, S. P. 1993. Diabetes mellitus and free radicals. Free radicals, transition, metals and oxidative mellitus and complication.Br. Med. Bull; 49: 264-652.
 
[21]  Prasad, S. K., Kulshreshtha, A. and Qureshi, T. N. 2009. Antidiabetic Activity of Some Herbal Plants in Streptozotocin Induced Diabetic Albino Rats. Pakistan Journal of Nutrition; 8 (5): 551-557.
 
[22]  Rohilla, A. and Ali, S., 20I2. Alloxan induced diabetes: mechanisms and effects. Int. J. Res. Pharma. Biomed Sci. 3: 819-823.
 
[23]  Dhanesha, N., Joharapurkar, A., Shah, G., Kshirsagar, S., Patel, V., Patel, K., Jain, M., 20I3. Treatment with exendin-4 improves the antidiabetic efficacy and reverses hepatic steatosis in glucokinase activator treated db/db mice. Eur. J. Pharmacol.714: 188-192.
 
[24]  El-Esawy, B.H., Alghamdy, A.N., El Askary, A., Elsayed, E.M., 20I6. Histopathological evaluation of the pancreas following administration of paricalcitol in alloxan-induced diabetic wistar rats. World. J. Pharm. Pharmac. Sci. 5: 189-198.
 
[25]  El-Esawy, B.H., Alghamdy, A.N., El Askary, A., Elsayed, E.M., 20I6. Histopathological evaluation of the pancreas following administration of paricalcitol in alloxan-induced diabetic wistar rats. World. J. Pharm. Pharmac. Sci. 5: 189-198.
 
[26]  Ho, E., Ning, Q., Yu, H. T., Weimin, L. and Tammy, M. B. 200I. Dietary zinc supplementation inhibits NFkB activation and protects againstchemically induced diabetes in CDI mice. Exp. Bio and Medicine; 226: 103-111.
 
[27]  Meghana K, Sanjeev G, Ramesh B. 2007. Curcumin prevents streptozotocin-induced islet damage by scavenging free radicals: a prophylactic and protective role. European Journal of Pharmacology. 577(1-3): 183-191.
 
[28]  Kanitkar M, Gokhale K, Galande S, Bhonde RR. 2008. Novel role of curcumin in the prevention of cytokine-induced islet death in vitro and diabetogenesis in vivo. British Journal of Pharmacology. I55(5): 702-7I3.
 
[29]  Kanitkar M, Bhonde RR. 2008. Curcumin treatment enhances islet recovery by induction of heat shock response proteins, Hsp70 and heme oxygenase-I, during cryopreservation. Life Sciences. 82(3-4): 182-189.
 
[30]  Zafar KS, Inayat-Hussain SH, Siegel D, Bao A, Shieh B, Ross D. 2006. Overexpression of NQOI protects human SK-N-MC neuroblastoma cells against dopamine-induced cell death. Toxicology Letters. 166(3): 261-267.
 
[31]  Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S. 2009. Induction of antioxidant enzymes by curcumin and its analogues in human islets: implications in transplantation. Pancreas. 38(4): 454-460.
 
[32]  Shatadal, G.; Sharmistha, B.; Parames C.S. 20I5. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease. Food and Chemical Toxicology. 83: 111-124.