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American Journal of Medical Sciences and Medicine. 2019, 7(4), 156-161
DOI: 10.12691/ajmsm-7-4-3
Open AccessArticle

Immunomodulatory Effect of Carica Papaya and Actinidia Delicosa on Methotrexate Induced Immunosuppression in Male Rats

Amina A. Bedawy1, , Ibrahim A. Ibrahim1, Sherif Y. Saleh1, Abeir A. Shalaby1 and Marwa A. El-Beltagy1

1Department of Biochemistry, College of Veterinary Medicine, University of Suez Canal, Egypt

Pub. Date: November 12, 2019

Cite this paper:
Amina A. Bedawy, Ibrahim A. Ibrahim, Sherif Y. Saleh, Abeir A. Shalaby and Marwa A. El-Beltagy. Immunomodulatory Effect of Carica Papaya and Actinidia Delicosa on Methotrexate Induced Immunosuppression in Male Rats. American Journal of Medical Sciences and Medicine. 2019; 7(4):156-161. doi: 10.12691/ajmsm-7-4-3


This study was carried out to explore the effects of alcoholic extract of Carica papaya fruit (C. papya) and Actinidia delicosa (Kiwi) fruit on some immunological, proinflammatory and anti-inflammatory biomarkers in methotrexate (MTX) treated rats. Forty adult male rats were weighted (130-160gm) and equally allocated into four groups and treated daily for 8weeks with orally as follow: Group G1: (control group) received tap water, G2: rats in this group were injected (20mg/kg /B.W) of MTX in 5thweek, G3: were orally received daily (400 mg/kg /B.W) of Carica papaya fruit alcoholic extract and injected (20mg/kg /B.W) of MTX in 5thweek , G4: were orally received daily (400mg/kg /B.W) of Actinidia delicosa fruit alcoholic extract and were injected (20mg/kg /B.W) of MTX in 5thweek. At the end of experiment, all animals were sacrificed and blood was drawn from median canthus of eye for measuring Serum immunoglobulin M, Serum immunoglobulin G, TNFα, interleukin-10 and C – reactive protein concentrations. Liver tissue collected in liquid nitrogen for Real Time PCR of IL1β and IL6 mRNA expression levels. Spleen tissue collected for histopathological examination and immunohistochemistry of TNFα expression levels. The results of the current study revealed A significant increase in serum serum IgM, IgG and IL10 as well as significant decrease in serum CRP concentrations and TNFα concentrations , mRNA expression levels of IL1β IL6 and immunohistochemical expression of TNFα were return to normal following extract administration comparing to the immune-suppression effect of MTX. Histopathological examination of spleen proved the protective effect of C.papaya and Kiwi fruit extract against necrosis and degenerative changes caused by MTX injection. Immunohistochemically, the expression level of TNFα in spleen tissue was decreased in Carica papaya fruit and Actinidia delicosa fruit groups when compared to MTX treated group. Conclusion, alcoholic extract of Carica papaya fruit and Actinidia delicosa fruit compete the side effects of MTX correlated with immune suppressive and pro-inflammatory effect.

methotrexate tumor necrotic factor alpha c-reactive protein

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