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American Journal of Microbiological Research

ISSN (Print): 2328-4129

ISSN (Online): 2328-4137


Content: Volume 1, Issue 1


Biodegradation of Dimethylformamide Using Bacillus Subtilis

1PG Department of Immunology and Microbiology, The American College, Madurai, India

2PG and Research Department of Zoology, The American College, Madurai, India

American Journal of Microbiological Research. 2013, 1(1), 10-15
DOI: 10.12691/ajmr-1-1-3
Copyright © 2013 Science and Education Publishing

Cite this paper:
R. Vidhya, A.J. Thatheyus. Biodegradation of Dimethylformamide Using Bacillus Subtilis . American Journal of Microbiological Research. 2013; 1(1):10-15. doi: 10.12691/ajmr-1-1-3.

Correspondence to: R. Vidhya, PG Department of Immunology and Microbiology, The American College, Madurai, India. Email:


The present study investigated the potential of the chosen bacterium, a natural isolate Bacillus subtilis isolated from textile industry effluent (textile effluent adapted bacterium) and the isolate was found to be more efficient in degrading DMF based on the assessment of physico-chemical parameters like pH, turbidity, carbon dioxide and ammonia released during the degradation process. DMF degradation has led to the accumulation of ammonia and dimethylamine contributing to the increase of pH of the medium from 7.0 to 9.2. Increase in turbidity and biomass was also observed during the treatment. The maximum release of carbon dioxide and ammonia was found during the degradation of 100µl of DMF. HPLC analysis for 200µl of DMF degradation by the isolate showed peaks with different retention times. Thus the results indicated that the isolate was able to degrade DMF found in the textile industrial effluents.



[1]  APHA, Standard methods for the examination of water and wastewater, 18th edition, 1992.
[2]  Bromley-Challenor, K.C.A., Caggiano, N. and Knapp. J.S, “Bacterial growth on N,N-dimethylformamide: Implications for the biotreatment of industrial wastewater”, Journal of Industrial Microbiology and Biotechnology, 25. 8-16. 2002.
[3]  Cooper, P., Color in dye house effluent. Bradford Society of Dyers and Colourists, Bradford, on behalf of the Dyers' Company Publications Trust, 1995.
[4]  Dojlido, J.R, Investigations of biodegradability and toxicity of organic compounds. Washington, DC, US Environmental Protection Agency (EPA-600/2-79-163) [cited in Howard, 1993], 1979.
[5]  Eaton, A.D., Clesceri, L.S. and Greenberg, A.L, Standard examination of water and wastewater, 19th Edition, 4.17, 1995.
Show More References
6]  European Chemicals Bureau, Trimethylamine. IUCLID (International Uniform Chemical Information Database), 1996a.
7]  European Chemicals Bureau, Dimethylamine. IUCLID (International Uniform Chemical Information Database), 1996b.
8]  Ghisalba, O., Cevey, P., Kuenzi, M. and Schär, H.P, “Biodegradation of chemical waste by specialized methylotrophs, and alternative to physical methods of waste disposal”, Conserv Recycl, 8. 47-71. 1985.
9]  Howard, P.H, Handbook of Environmental Fate and Exposure Data for Organic Chemicals 7(2), Chelsea, MI: Lewis Publishers Inc, 1993.
10]  IARC, Evaluations-in press. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. Lyon, International Agency for Research on Cancer, 1988.
11]  IARC, Dimethylformamide. In: Some organic solvents, resin monomers and related compounds, pigments and occupational exposures in paint manufacture and painting. Lyon, International Agency for Research on Cancer, 171-197. 1989.
12]  IARC, Dimethylformamide. In: Re-evaluation of some organic chemicals, hydrazine and hydrogen peroxide. Lyon, International Agency for Research on Cancer, 545-574. 1999.
13]  Kennedy, G.L, and Sherman, H., “Acute and subchronic toxicity of dimethylformamide and dimethylacetamide following various routes of administration”, Drug and chemical toxicology, 9. 147-170. 1986.
14]  Long, G., and Meek. M.E, Concise international chemical assessment. Document 31. World Health Organization, Geneva, 1-61. 2001.
15]  Olayinka, K.O, and Alo, B.I, “Studies on industrial pollution in Nigeria: The effect of Textile effluent on the quality of ground waters in some parts Lagos”, Nigerian Journal of Health and Biomedical Sciences, 22-25. 2004.
16]  Pellizzari, E.O, The measurement of carcinogenic vapors in ambient atmosphere. Research Triangle Park, NC, US Environmental Protection Agency, Washington DC, 1977.
17]  Redlich, C., Beckett, W.S., Sparer, J., Barwick, K.W., Riely, C.A., Millersigal, H., Shalat, S.L. and Cullen, M.R, “Liver disease associated with occupational exposure to the solvent dimethylformamide”, Annals of Internal medicine, 108. 680-686. 1988.
18]  Scailteur, V., and Lauwerys, R.R, “Dimethylformamide (DMF) Hepatotoxicity”, Toxicology, 43. 231-38. 1987.
19]  Smyth, H.F., and Carpenter, C.P, “Further experience with the range-finding test in the industrial toxicology laboratory”, Journal of industrial hygiene and toxicology, 30(1). 63-68. 1948.
20]  Stolz, A., “Basic and Applied Aspects in the Microbial Degradation of Azo Dyes”, Applied microbial biotechnology, 56. 69-80. 2001.
21]  US EPA, United States Environmental Protection Agency, Superfund Public Health Evaluation Manual. EPA/5401/1-86/060, US Environmental Protection Agency, Washington DC, 1986.
22]  Veeranagouda, Y., Emmanuel Paul, P.V., Gorla, P., Siddavattam, D., and Karegoudar, T.B, “Complete mineralisation of dimethylformamide by Ochrobactrum sp. DGVK1 isolated from the soil samples collected from the coalmine leftovers”, Applied Microbiology and Biotechnology, 71. 369-375. 2006.
Show Less References


Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates

1Department of Microbiology, Barkatullah University, Bhopal, India

2Department of Oral and Maxillofacial Surgery, People’s Dental Academy, Bhopal, India

American Journal of Microbiological Research. 2013, 1(1), 4-9
DOI: 10.12691/ajmr-1-1-2
Copyright © 2013 Science and Education Publishing

Cite this paper:
Rakesh Kumar Patidar, Mithilesh Kumar Gupta, Vinod Singh. Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates. American Journal of Microbiological Research. 2013; 1(1):4-9. doi: 10.12691/ajmr-1-1-2.

Correspondence to: Vinod Singh, Department of Microbiology, Barkatullah University, Bhopal, India. Email:


Enterococcus faecalis is a Gram-positive member of human gastrointestinal flora that is in recent years emerging as an important cause of endodontic infections. In this study, we have investigated the occurrence of virulence determinants and antibiotic susceptibility pattern of E. faecalis isolates, originating from root canals of apical periodontitis. Among 52 E. faecalis isolates, 32 (61.5%) isolates produced hemolysin on blood agar while all (100%) isolates showed hemolysin production on BHI-GA ((BHI medium supplemented with 1% glucose and 0.03% L-arginine), 18 (34.6%) produced gelatinase, 38 (73%) produced caseinase, no hemagglutination was observed in E. faecalis isolates, whereas all 52 (100%) resistant to serum and formed biofilm. Antibiotic susceptibility results showed that all (100%) E. faecalis isolates were susceptible to amoxicillin, amoxicillin/clavulanate, and vancomycin. Whereas, 32 (61.5%) E. faecalis isolates were resistant to chloramphenicol, 30 (57.6%) isolates were resistant to ciprofloxacin, 39 (75%) isolates were resistant to erythromycin, and 34 (65.3%) isolates were resistant to tetracycline. Multi-drug resistance was observed in 16 (30.7%) isolates of E. faecalis to chloramphenicol, ciprofloxacin, erythromycin and tetracycline antibiotics. These findings demonstrate the presence of putative virulence determinants in E. faecalis isolates originating from root canal and suggest amoxicillin, amoxicillin/clavulanate, and vancomycin as more effective than other antibiotics tested.



[1]  Jett B.D., Huycke M.M., Gilmore M.S. “Virulence of enterococci”. Clin Microbiol Rev, 7. 462-78. 1994.
[2]  Sedgley C., Buck G., Appelbe O. “Prevalence of Enterococcus faecalis at multiple oral sites in endodontic patients using culture and PCR”. J Endod, 32. 104-09. 2006.
[3]  Johnson E.M., Flannagan S.E., Sedgley C.M. “Coaggregation interactions between oral and endodontic Enterococcus and bacterial species isolated from persistent apical periodentitis”. J Endod, 32. 946-50. 2006.
[4]  Pinheiro E.T., Gomes B.P., Ferraz C.C., Sousa E.L., Teixeira F.B., Souza- Filho F.J. “Microorganisms from canals of root-filled teeth with periapical lesions”. Int Endod J, 36. 1-11. 2003.
[5]  Evans M., Davies J.K., Sundqvist G., Figdor D. “Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide”. Int Endod J, 35. 221-28. 2002.
Show More References
6]  Rocas I.N., Siqueira J.F., Santos K.R.N. “Association of Enterococcus faecalis with different forms of periradicular diseases”. J Endod, 30. 315-20. 2004.
7]  Patidar R.K, Gupta M.K, Singh V. “Virulence of Enterococcus faecalis and impact of genome-wide approaches”. South Asian J Exp Biol, 1. 16-24. 2011.
8]  Love R.M. “Enterococcus faecalis: a mechanism for its role in endodontic failure”. Int Endod J, 34. 399-05. 2001.
9]  Lee W., Lim S., Son H., Bae K. “Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes”. J Endod, 30. 209-12. 2004.
10]  Nair P.N.R. “On the causes of persistent apical periodontitis: a review”. Int End J, 39. 249-81. 2006.
11]  Svensäter G., Bergenholtz G. “Biofilms in endodontic infections”. Endod Top, 9. 27-36. 2004.
12]  Costerton J.W., Stewart P.S. “Biofilms and device-related infections”. In: Nataro P.J., Balser M.J., Cunningham-Rundels S., eds. Persistent Bacterial Infections. Washington, 2000. 423-39.
13]  Hancock H.H., Sigurdsson A., Trope M., Moiseiwitsch J. “Bacteria isolated after unsuccessful endodontic treatment in a North American population”. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91. 579-86. 2001.
14]  Molander A., Reit C., Dahle´n G., Kvist T. “Microbiological status of root-filled teeth with apical periodontitis”. Int Endod J, 31. 1-7. 1998.
15]  Peciuliene V., Reynaud A.H., Balciuniene I., Haapasalo M. “Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis”. Int Endod J, 34. 429-34. 2001.
16]  Murray B.E. “The life and times of the enterococci”. Clin Microbiol Rev, 3. 46-65. 1990.
17]  Gomes B.P.F.A., Pinheiro E.T., Gade-Neto C.R., Sousa E.L., Ferraz C.C., Zaia A.A., Teixeira F.B., Souza-Filho F.J. “Microbiological examination of infected dental root canal”. Oral Microbiol Immunol, 19. 71-76. 2004.
18]  Reynaud af Geijersstam A., Culak R., Molenaar L., Chattaway M., Røslie E., Peciuliene V., Haapasalo M., Shah H.N. “Comparative analysis of virulence determinants and mass spectral profiles of Finnish and Lithuanian endodontic Enterococcus faecalis isolates”. Oral Microbiol Immunol, 22. 88-94. 2007.
19]  Booth M.C., Bogie C.P., Sahl H.G., Siezen R.J., Hatter K.L., Gilmore M.S. “Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibiotic”. Mol Microbiol, 21. 1175-84. 1996.
20]  Vergis E.N., Shankar N., Chow J.W., Hayden M.K., Snydman D.R., Zervos M.J., Linden P.K., Wagener M.M., Muder R.R. “Association between the presence of enterococcal virulence factor gelatinase, hemolysin, and enterococcal surface protein and mortality among patients with bacteremia due to Enterococcus faecalis”. Clin Infect Dis, 35. 570-75. 2003.
21]  Kurl D.N., Haataja S., Finne J. “Hemagglutination activities of group B, C, D and G streptococci: demonstration of novel sugar specific cell-binding activities in Streptococcus suis”. Infect Immun, 57. 384-89. 1989.
22]  Archimbaud C., Shankar N., Forestier C., Baghdayan A., Gilmore M.S., Charbonne F., Joly B. “In vitro adhesive properties and virulence factors of Enterococcus faecalis strains”. Res Microbiol, 153. 75-80. 2002.
23]  Pelkonen S., Finne J. “A rapid turbidmetric assay for the study of serum sensitivity of Escherichia coli”. FEMS Microbiol Lett, 42. 55-57. 1987.
24]  Taylor P.W. “Bactericidal and bacteriolytic activity of serum against gram-negative bacteria”. Microbiol Rev, 47. 46-83. 1983.
25]  Wakimoto N., Nishi J., Sheikh J., Nataro J.M., Sarantuya J., Iwashita M., Manago K., Tokuda K., Yoshinaga M., Kawano Y. “Quantitative biofilm assay using microtiter plate to screen for enteroaggretative Escherichia coli”. Am J Trop Med Hyg, 71. 687-90. 2004.
26]  Pinheiro E.T., Anderson M.J., Gomes B.P.F.A., Drucker D.B. “Phenotypic and genotypic identification of enterococci isolated from canals of root-filled teeth with periapical lesions”. Oral Microbiol Immunol, 21. 137-44. 2006.
27]  Guven Kayaoglu, Orstakavik D. “Virulence of Enterococcus faecalis: relationship to endodontic disease”. Crit Rev Oral Biol Med, 15. 308-20. 2004.
28]  Sedgley C., Nagel A., Dahlen G., Reit C., Molander A. “Real-time quantative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals”. J Endod, 32. 173-77. 2003.
29]  Sunde P.T., Olsen I., Debelian G.J., Tronstad L. “Microbiota of periapical lesions refractory to endodontic therapy”. J Endod, 28. 304-10. 2002.
30]  Furumura M.T., Figueiredo P.M.S., Carbonella G.V., da Costa Darini A.L., Yano T. “Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources”. Braz J Microbiol, 37. 230-36. 2006.
31]  Sedgley C.M., Lennan S.L., Clewell D.B. “Prevalence, phenotype and genotype of oral enterococci”. Oral Microbiol Immunol, 19. 95-01. 2004.
32]  Waters C.M., Antiporta M.H., Murray B.E., Dunny G.M. “Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins”. J Bacteriol, 185. 3613-23. 2003.
33]  Sedgley C.M., Nagel A.C., Shelburne C.E., Clewell D.B., Appelbe O., Molander A. “Quantitative real-time PCR detection of oral Enterococcus faecalis in humans”. Arch Oral Biol, 50. 575-83. 2005.
34]  Hubble T.S., Hatton J.F., Nallapareddy S.R., Murray B.E., Gillespie M.J. “Influence of Enterococcus faecalis proteases and the collagen binding protein, Ace, on adhesion to dentin”. Oral Microbiol Immunol, 18. 121-26. 2003.
35]  Podschun R., Ullmann U. “Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors”. Clin Microbiol Rev, 11. 589-03. 1998.
36]  Costerton J.W. “Introduction to biofilm”. Int J Antimicrobial Agents, 11. 217-21. 1999.
37]  Di Martino P., Cafferini N., Joly B., Darfeuille-Michaud A. “Klebsiella pneumoniae type 3 pili facilitate adherence and biofilm formation on abiotic surfaces”. Res Microbiol, 154. 9-16. 2002.
38]  Kritish C.J., Li Y.H., Cvithkovitch D.G. Dunny G.M. “Esp independent biofilm formation by Enterococcus faecalis”. J Bacteriol, 186. 154-63. 2004.
39]  Coque T.M., Tomayko J.F., Ricke S.C., Okhyusen P.C., Murray B.E. “Vancomycin-resistant enterococci from nosocomial, community, and animal sources in the United States”. Antimicrob Agents Chemother, 40. 2605-09. 1996.
40]  Dunny G.M., Leonard B.A., Hedberg P.J. “Pheromoneinducible conjugation in Enterococcus faecalis: interbacterial and host–parasite chemical communication”. J Bacteriol, 177. 871-76. 1995.
41]  Galli D., Lottspeich F., Wirth R. “Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1”. Mol Microbiol, 4. 895-04. 1990.
Show Less References


Stability of Tyrosinase Enzyme from Funalia Trogii

1Department of Chemistry (Biochemistry), Faculty of Science, Hacettepe University, Ankara, Turkey

2Department of Biology (Biotechnology), Faculty of Science, Hacettepe University, Ankara, Turkey

American Journal of Microbiological Research. 2013, 1(1), 1-3
DOI: 10.12691/ajmr-1-1-1
Copyright © 2013 Science and Education Publishing

Cite this paper:
DARYOUSH MAJIDI, NİLÜFER AKSÖZ. Stability of Tyrosinase Enzyme from Funalia Trogii. American Journal of Microbiological Research. 2013; 1(1):1-3. doi: 10.12691/ajmr-1-1-1.

Correspondence to: DARYOUSH MAJIDI, Department of Chemistry (Biochemistry), Faculty of Science, Hacettepe University, Ankara, Turkey. Email:


Tyrosinases are widely distributed in nature; These enzymes are known as type 3 copper proteins having a diamagnetic spin-coupled copper pair in the active centre. In this study, the objective was to produce stable tyrosinase enzyme efficiently and determine stability of enzyme from an alternative fungal soruce, Funalia trogii. Temperature and pH stabilities of the crude extract of enzyme were studied and it was concluded that crude extract of tyrosinase was stable at 73ºC . pH stability of tyrosinase enzyme was among 3,5-7 ranges.



[1]  Lerch, K., "Neurospora tyrosinase: structural, spectroscopic and catalytic properties", Molecular and Cellular Biochemistry, 52(2): 125-138,1983.
[2]  Robb, D.A., "Tyrosinase", Copper Proteins and Copper Enzymes, 2: 207-241,1984.
[3]  Rodriguez-Lopez, J.N., et al., "Analysis of a kinetic model for melanin biosynthesis pathway", Journal of Biological Chemistry, 267(6): 3801-3810,1992.
[4]  Seo, S.Y., V.K. Sharma, and N. Sharma, "Mushroom tyrosinase: Recent prospects", Journal of agricultural and food chemistry, 51(10): 2837-2853,2003.
[5]  Martinez, M.V. and J.R. Whitaker, "The biochemistry and control of enzymatic browning", Trends in Food Science and Technology, 6(6): 195-200,1995.
Show More References
6]  MAJIDI D., A.N., "Optimization of Medium Components and Cultural Variables for Enhanced Production of Tyrosinase by Funalia trogii", European International Journal of Science and Technology, 1(2): 41-47,2012.
7]  Sun, W.-Q.a.P., G.F., "Tyrosinase-containing chitosan gels: A combined catalyst and sorbent for selective phenol removal", Biotechnology and Bioengineering, 51: 79-86, 1996.
8]  Da-You Xu, Y.Y., Zhen Yang, "Activity and stability of cross-linked tyrosinase aggregates in aqueous and nonaqueous media", Journal of Biotechnology, 152: 30-36, 2011.
9]  Aytar, B.S., Bakir, U., "Preparation of cross-linked tyrosinase aggregates", Process Biochem, 43: 125-131, 2008.
10]  Montoro-García, S., Gil-Ortiz, F., Navarro-Fernández, et al., "Improved cross-linked enzyme aggregates for the production of desacetyl-lactam intermediates", Bioresource Technology,101(1): 331-336, 2010.
Show Less References