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Article

Biodegradation of Dimethylformamide Using Bacillus Subtilis

1PG Department of Immunology and Microbiology, The American College, Madurai, India

2PG and Research Department of Zoology, The American College, Madurai, India


American Journal of Microbiological Research. 2013, 1(1), 10-15
DOI: 10.12691/ajmr-1-1-3
Copyright © 2013 Science and Education Publishing

Cite this paper:
R. Vidhya, A.J. Thatheyus. Biodegradation of Dimethylformamide Using Bacillus Subtilis . American Journal of Microbiological Research. 2013; 1(1):10-15. doi: 10.12691/ajmr-1-1-3.

Correspondence to: R. Vidhya, PG Department of Immunology and Microbiology, The American College, Madurai, India. Email: vidvirgo@gmail.com

Abstract

The present study investigated the potential of the chosen bacterium, a natural isolate Bacillus subtilis isolated from textile industry effluent (textile effluent adapted bacterium) and the isolate was found to be more efficient in degrading DMF based on the assessment of physico-chemical parameters like pH, turbidity, carbon dioxide and ammonia released during the degradation process. DMF degradation has led to the accumulation of ammonia and dimethylamine contributing to the increase of pH of the medium from 7.0 to 9.2. Increase in turbidity and biomass was also observed during the treatment. The maximum release of carbon dioxide and ammonia was found during the degradation of 100µl of DMF. HPLC analysis for 200µl of DMF degradation by the isolate showed peaks with different retention times. Thus the results indicated that the isolate was able to degrade DMF found in the textile industrial effluents.

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References

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Article

Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates

1Department of Microbiology, Barkatullah University, Bhopal, India

2Department of Oral and Maxillofacial Surgery, People’s Dental Academy, Bhopal, India


American Journal of Microbiological Research. 2013, 1(1), 4-9
DOI: 10.12691/ajmr-1-1-2
Copyright © 2013 Science and Education Publishing

Cite this paper:
Rakesh Kumar Patidar, Mithilesh Kumar Gupta, Vinod Singh. Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates. American Journal of Microbiological Research. 2013; 1(1):4-9. doi: 10.12691/ajmr-1-1-2.

Correspondence to: Vinod Singh, Department of Microbiology, Barkatullah University, Bhopal, India. Email: vsingh3@rediffmail.com

Abstract

Enterococcus faecalis is a Gram-positive member of human gastrointestinal flora that is in recent years emerging as an important cause of endodontic infections. In this study, we have investigated the occurrence of virulence determinants and antibiotic susceptibility pattern of E. faecalis isolates, originating from root canals of apical periodontitis. Among 52 E. faecalis isolates, 32 (61.5%) isolates produced hemolysin on blood agar while all (100%) isolates showed hemolysin production on BHI-GA ((BHI medium supplemented with 1% glucose and 0.03% L-arginine), 18 (34.6%) produced gelatinase, 38 (73%) produced caseinase, no hemagglutination was observed in E. faecalis isolates, whereas all 52 (100%) resistant to serum and formed biofilm. Antibiotic susceptibility results showed that all (100%) E. faecalis isolates were susceptible to amoxicillin, amoxicillin/clavulanate, and vancomycin. Whereas, 32 (61.5%) E. faecalis isolates were resistant to chloramphenicol, 30 (57.6%) isolates were resistant to ciprofloxacin, 39 (75%) isolates were resistant to erythromycin, and 34 (65.3%) isolates were resistant to tetracycline. Multi-drug resistance was observed in 16 (30.7%) isolates of E. faecalis to chloramphenicol, ciprofloxacin, erythromycin and tetracycline antibiotics. These findings demonstrate the presence of putative virulence determinants in E. faecalis isolates originating from root canal and suggest amoxicillin, amoxicillin/clavulanate, and vancomycin as more effective than other antibiotics tested.

Keywords

References

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Article

Stability of Tyrosinase Enzyme from Funalia Trogii

1Department of Chemistry (Biochemistry), Faculty of Science, Hacettepe University, Ankara, Turkey

2Department of Biology (Biotechnology), Faculty of Science, Hacettepe University, Ankara, Turkey


American Journal of Microbiological Research. 2013, 1(1), 1-3
DOI: 10.12691/ajmr-1-1-1
Copyright © 2013 Science and Education Publishing

Cite this paper:
DARYOUSH MAJIDI, NİLÜFER AKSÖZ. Stability of Tyrosinase Enzyme from Funalia Trogii. American Journal of Microbiological Research. 2013; 1(1):1-3. doi: 10.12691/ajmr-1-1-1.

Correspondence to: DARYOUSH MAJIDI, Department of Chemistry (Biochemistry), Faculty of Science, Hacettepe University, Ankara, Turkey. Email: majididaryoush@gmail.com

Abstract

Tyrosinases are widely distributed in nature; These enzymes are known as type 3 copper proteins having a diamagnetic spin-coupled copper pair in the active centre. In this study, the objective was to produce stable tyrosinase enzyme efficiently and determine stability of enzyme from an alternative fungal soruce, Funalia trogii. Temperature and pH stabilities of the crude extract of enzyme were studied and it was concluded that crude extract of tyrosinase was stable at 73ºC . pH stability of tyrosinase enzyme was among 3,5-7 ranges.

Keywords

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