American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
American Journal of Microbiological Research. 2018, 6(4), 181-186
DOI: 10.12691/ajmr-6-4-6
Open AccessArticle

Detection, Identification & Sequencing of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) among Sudanese Patients

Ibrahim H.S1, , Kafi S.K1, Musa H.A2, Karsani M.S3 and Mahadi A.M4

1Faculty of Medical Laboratory Science, The National Ribat University

2Research Laboratory, Faculty of Medical Laboratory Science, The National Ribat University

3Faculty of Medical Laboratory Science, Karari University

4Department of Parasitology & Molecular Biology, Institute of Endemic Disease

Pub. Date: November 23, 2018

Cite this paper:
Ibrahim H.S, Kafi S.K, Musa H.A, Karsani M.S and Mahadi A.M. Detection, Identification & Sequencing of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) among Sudanese Patients. American Journal of Microbiological Research. 2018; 6(4):181-186. doi: 10.12691/ajmr-6-4-6

Abstract

MERS-CoV virus is a newly emerged coronaviruses in KSA in 2012; followed by a lot of cases in the Middle East & other European, American & African countries. The goal of this study is to detect, identify & sequencing of MERS-CoV among Sudanese patients suffering from respiratory diseases by using the orf1a with upE gene for the virus detections. Phylogenetic analysis of upE gene of MERS-CoV was done using ViPR. MERS-CoV virus seems to be highly prevalent among Sudanese population especially among patients from Al-Shaab hospital than individuals from the International Khartoum Airport (86.8% & 12.1% vs 66.6% & 9.0%) respectively; this attributed to the fact that Al-Shaab hospital participants were symptomatic, having severe respiratory symptoms (possibly caused by coronaviruses). Phylogenetic analysis of the isolated viruses from Khartoum International Airport participant showed genetic similarity with KSA while Al-Shaab Teaching Hospital individuals showed genetic similarity with Thailand. Viral genomic were deposit in gene bank with those accession N.o: KY794400.1-KY794403.1 and MF133448-MF133457.

Keywords:
MERS-CoV sequence Open Reading Frame (ORF) ViPR. RI

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  www.Middle-East-respiratory-syndrome-coronavirus.htm. Middle East respiratory syndrome coronavirus From Wikipedia, the free encyclopedia (2013).
 
[2]  Wang Y, Liub D, Shid W, Lua R, Wanga W, Zhaoa Y, Denga Y, Zhoua W, Rene H, Wub J, Wangf Y, Wua G, Gaoa F.G, Tana W (2015). Origin and Possible Genetic Recombination of the Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China: Phylogenetics and Coalescence Analysis. 8 September 2015 mBio vol. 6 no. 5 e01280-15.
 
[3]  Ali M.A, Shehata M.M, Gomaa M.R, Kandeil A, El-Shesheny R, Kayed A.S, et al. Systematic, active surveillance for Middle East respiratory syndrome coronavirus in camels in Egypt. emerging microbes & infections (2017) 6.
 
[4]  Cabeça T.K, Passos A.M, Granato C, Bellei N. Human coronavirus ocurrence in different populations of Sao Paulo: A comprehensive nine-year study using a pancoronavirus RT-PCR assay. Braz J Microbiol. 2013; 44(1): 335-339.
 
[5]  Burrel S, Jaureguiberry S, Caumes E, Aubry A, Agut H, Boutolleau D (2016). Microbiological evaluation of respiratory tract infections in pilgrims returning from countries affected by Middle East respiratory syndrome coronavirus (MERS-CoV). September 2016Volume 82, Supplement, Page S54.
 
[6]  Gerna G, Campanini G, Rovida F, Percivalle E, Sarasini A, Marchi A, et al. Genetic variability of human coronavirus OC43-, 229E-, and NL63-like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients. j med virol. (2006). 78(7): 938-49.
 
[7]  Moës E, Vijgen L, Keyaerts E, Zlateva K, Li S, Maes P, et al. A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium. bmc infectious diseasesbmc (2005).5:6. http://A novel pancoronavirus RT-PCR assay-frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium-BMC Infectious Diseases-Full Text.html.
 
[8]  Mahony J.B. Detection of respiratory viruses by molecular methods. clin. microbiol. rev (2008). vol. 21(4): 716-747.
 
[9]  Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, Al-Rabiah FA, Al-Hajjar S, Al-Barrak A, et al. Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. lancet infect dis (2013). pii: S1473-3099(13)70204-4.