American Journal of Microbiological Research
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American Journal of Microbiological Research. 2018, 6(2), 57-62
DOI: 10.12691/ajmr-6-2-4
Open AccessArticle

Association of H.pylori cagA Gene with Duodenal Ulcer & Gastric Carcinoma in Bangladeshi Patients

Ritu Saha1, , Sharmeen Ahmed2, Humayun Sattar2, Maksuma Begum3, Bhuiyan Mohammad Mahtab Uddin4, Ahmed Abu Saleh2 and Ruhul Amin Miah2

1Department of Microbiology, Bashundhara Ad-din Medical College & Hospital, Dhaka, Bangladesh

2Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

3Department of Microbiology, Shahid Monsur Ali Medical College, Dhaka, Bangladesh

4Enam Medical College, Dhaka, Bangladesh

Pub. Date: April 04, 2018

Cite this paper:
Ritu Saha, Sharmeen Ahmed, Humayun Sattar, Maksuma Begum, Bhuiyan Mohammad Mahtab Uddin, Ahmed Abu Saleh and Ruhul Amin Miah. Association of H.pylori cagA Gene with Duodenal Ulcer & Gastric Carcinoma in Bangladeshi Patients. American Journal of Microbiological Research. 2018; 6(2):57-62. doi: 10.12691/ajmr-6-2-4

Abstract

Background: Prolong infection with Helicobacter pylori may lead to chronic inflammation of gastroduodenal mucosa which in turns develops into severe diseases like peptic ulcer and gastric carcinoma. Bacterial virulence factor Cytotoxin Associated gene (cagA) is found to be responsible for developing such severe diseases in different countries. So this study was conducted to assess the relationship between occurrence of several gastroduodenal diseases and the presence of H. pylori cagA gene in Bangladeshi patients. Methods: Endoscopic gastroduodenal biopsy sample of 113 dyspeptic patients from different districts of Bangladesh were studied. H. pylori infection was detected by Rapid urease test, PCR of ureC gene and histological staining (Geimsa). Gastroduodenal disease was diagnosed by histopathological examination and cagA gene was detected by PCR. Result: H. pylori infection was identified among 48% (54/113) patients. Fifty seven percent of H. pylori infected patients were found to be cagA gene positive. cagA gene is significantly associated with Duodenal ulcer (p= .024) and Gastric carcimoma (p < .001). However, a further larger study is required to confirm this finding.

Keywords:
H. pylori infection cagA gene duodenal ulcer gastric carcinoma

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