American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2018, 6(1), 33-37
DOI: 10.12691/ajmr-6-1-5
Open AccessArticle

Induction of Abscess by Non-abscess Producer Staphylococci Using Staphylococcal Fatty Acid Modifying Enzyme (FAME)

Raga I. O.1, , Gameel A. A.2 and El-Sanousi S. M.3

1Department of Microbiology, Faculty of Veterinary Science, University of Nyala, P. O. Box 155, Nyala Sudan

2Department of Pathology, Faculty of Veterinary Medicine, University of Khartoum, P. O. Box 32, Khartoum Sudan

3Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, P. O. Box 32, Khartoum Sudan

Pub. Date: March 14, 2018

Cite this paper:
Raga I. O., Gameel A. A. and El-Sanousi S. M.. Induction of Abscess by Non-abscess Producer Staphylococci Using Staphylococcal Fatty Acid Modifying Enzyme (FAME). American Journal of Microbiological Research. 2018; 6(1):33-37. doi: 10.12691/ajmr-6-1-5

Abstract

Stahpylococcus epidermidis (S. epidermidis), S. warneri and S. aureus subsp. anaerobious were isolated from sheep abscess. Crude FAME was extracted from supernatant fluid of a 24hrs culture of S. aureus subsp. anaerobious grown in Trypticase Soy Broth at 37°C with continuous agitation. Fourteen heads of sheep were used. Three of them were inoculated with S. epidermidis + FAME and other three were inoculated with S. warneri + FAME. Eight animals were divided into four groups, two animals in each, used as control and inoculated with S. epidermidis, S. warneri, S. aureus subsp. anaerobious and FAME. All animals were inoculated subcutaneously at the neck region. After fourteen to eighteen days, sites inoculated with S. epidermidis + FAME, S. warneri + FAME and S. aureus subsp. anaerobious showed abscesses grossly then confirmed bateriologically and histopathologically, while those inoculated with S. epidermidis alone, S. warneri alone and FAME showed no abscess.

Keywords:
Stahpylococcus abscess fatty acid modifying enzyme

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