American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: Editor-in-chief: Apply for this position
Open Access
Journal Browser
American Journal of Microbiological Research. 2017, 5(4), 91-93
DOI: 10.12691/ajmr-5-4-4
Open AccessArticle

Antimicrobial Properties of Phenolic Extracts against Nosocomial Drug Resistant Organisms

Sambasivarao Yaragalla1, , Surya k krishnanbabu2, Jaisankar L Gopinath2, Buvan G Gengadharan2, Thanagam Brightline MK2 and Harnell Madhurin3

1Microbiology, Spartan Health Sciences University, Community Health and Research Centre of Spartan, St. Lucia

2Basic Science-5, Spartan Health Sciences University, St. Lucia

3Regional Plant Institute, V-fot, St. Lucia

Pub. Date: September 21, 2017

Cite this paper:
Sambasivarao Yaragalla, Surya k krishnanbabu, Jaisankar L Gopinath, Buvan G Gengadharan, Thanagam Brightline MK and Harnell Madhurin. Antimicrobial Properties of Phenolic Extracts against Nosocomial Drug Resistant Organisms. American Journal of Microbiological Research. 2017; 5(4):91-93. doi: 10.12691/ajmr-5-4-4


Nosocomial Multi Drug resistance microbial infection is a global concern nowadays. Due to its multi-drug resistant nature, treatment with conventional antibiotics does not assure desired clinical outcomes. Therefore, there is a need to find new compounds and/or alternative methods to get arsenal against the pathogen. Combination therapies using conventional antibiotics and phytochemicals fulfill both requirements [1]. A variety of essential oils have been screened for their antimicrobial activity [15]. The antimicrobial activity of plant-derived essential oils is the basis of many applications, especially in food preservation, aromatherapy and medicine. This present study demonstrates the efficacy of phenolic-phytochemical extract combinations from pine, coconut and oatmeal colloidal solution against multidrug resistant organisms such as Staphylococcus aureus (ATCC 43300), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 10231). The efficacy of the phytochemical combinations against these microbes were measured by estimating the inhibitory zone as produced by antibiotic sensitivity disc diffusion method on Mueller-Hinton agar. These results can be used as a support in doing further quantitative analysis in prevention and treatment of the common infectious diseases that is caused by these organisms [4].

phytochemicals antibiotic nosocomial drug resistance staphylococcus pseudomonas and candida

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


Figure of 2


[1]  Bhone Myint Kyaw, Shuchi arora, and Chu Sing Lim. 2012. Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus, Jul-Sep; 43(3): 938-945.
[2]  Aimé Gabriel Fankam, Jules-Roger Kuiate and Victor Kuete. 2017. Antibacterial and antibiotic resistance modulatory activities of leaves and bark extracts of Recinodindron heudelotii (Euphorbiaceae) against multidrug-resistant Gram-negative bacteria, BMC Complementary and Alternative MedicineBMC series – open, inclusive and trusted, 17: 168.
[3]  Biswajit Chakraborty, Anupam Nath, Himadri Saikia, Mahuya Sengupta. 2014. Bactericidal activity of selected medicinal plants against multidrug resistant bacterial strains from clinical isolates, Asian Pac J Trop Med, 7(Suppl 1): S435-S441.
[4]  Pandey R, Sambasivarao Y, Gurumurthy. 2013. Antibacterial Activity of Medicinal Plants against Pathogens from Extracts of Achyranthes Aspera. Med Aromat Plants 2: 135.
[5]  Mohtar M, Johari SA, Li AR, Isa MM, Mustafa S, Ali AM, Basri DF. 2009. Inhibitory and resistance-modifying potential of plant-based alkaloids against methicillin-resistant Staphylococcus aureus (MRSA), Curr Microbiol. Aug; 59(2): 181-6.
[6]  Foster, T.J. 2004. The Staphylococcus aureus “superbug”. J. Clin. Invest. 114 (12), 1693-1696.
[7]  Yamaoka T. 2007. The bactericidal effects of anti-MRSA agents with rifampicin and sulfamethoxazole-trimethoprim against intracellular phagocytized MRSA, J Infect Chemother. Jun; 13(3): 141-6.
[8]  Cottarel G, Wierzbowski J. 2007. Combination drugs, an emerging option for antibacterial therapy, Trends Biotechnol. Dec; 25(12): 547-55.
[9]  Entenza JM, Giddey M, Vouillamoz J, Moreillon P. 2010. In vitro prevention of the emergence of daptomycin resistance in Staphylococcus aureus and enterococci following combination with amoxicillin/clavulanic acid or ampicillin, Int J Antimicrob Agents. May; 35(5): 451-6.
[10]  Mouton JW. 1999. Combination therapy as a tool to prevent emergence of bacterial resistance, Infection. 27 Suppl 2: S24-8.
[11]  Cowan MM. 1999. Plant products as antimicrobial agents, Clin Microbiol Rev. Oct; 12(4):564-82.
[12]  Owoyale, J. A., G. A. Olatunji, and S. O. Oguntoye. 2005. “Antifungal and antibacterial activities of an alcoholic extract of Senna alata leaves.” Journal of Applied Sciences and Environmental Management, 9.3: 105-107.
[13]  Geetha K, Narayanan KR, Murugesan AG, 2010. Antimicrobial efficiency of Achyranthes aspera L. against selected pathogenic organisms. J Biosci Res 1: 187-190.
[14]  Singelton, V.R., Orthifer, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology 299, 152-178.
[15]  Cantrell CL, Fischer NH, Urbatsch L, McGuire MS, Franzblau SG. 1998. Antimycobacterial crude plant extracts from South, Central, and North America. Phytomedicine 5(2): 137-145.