American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2017, 5(2), 28-31
DOI: 10.12691/ajmr-5-2-1
Open AccessArticle

Operative Techniques, Treatment, and Precaution of Common Complications of Orthotopic Rat Liver Transplantation

Mao-Ming Tang1, Yun-Bing Wang2 and Jian-Ping Gong2,

1Department of Surgery, Wanzhou Hospital of TCM of Chongqing, Chongqing, China

2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China

Pub. Date: April 10, 2017

Cite this paper:
Mao-Ming Tang, Yun-Bing Wang and Jian-Ping Gong. Operative Techniques, Treatment, and Precaution of Common Complications of Orthotopic Rat Liver Transplantation. American Journal of Microbiological Research. 2017; 5(2):28-31. doi: 10.12691/ajmr-5-2-1

Abstract

Objective This study investigates operative techniques, treatment, and precaution for common complications of orthotopic liver transplantation (OLT) in rats. Methods OLT was performed in 110 rats through modified two-cuff method. Operative techniques were concluded, and causes, treatment, and precaution of common complications were analyzed. Results In operation phase, main causes for rat death included anesthesia, airway obstruction, pneumothorax, hemorrhagic shock, long anhepatic time, and air embolism. After surgery, main causes for rat death were as follows: stoma bleeding, infection, obstruction, and necrosis of biliary tract and poor hepatic function restoration. Conclusion Improving operative techniques, modifying operative methods, and familiarizing with common complications can reduce occurrence of complications, heighten operative successful rate, and establish more stable models of OLT in rats.

Keywords:
liver transplantation operative technique complication rat

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Li GL, Lin HM, Long TZ, Lv LH, Yu JD, Huang YH, Min J, Wan YL, “High incidence of biliary complications in rat liver transplantation: Can we avoid it? ” World J Gastroenterol, 17(26): 3140-3144, 2011.
 
[2]  Wang H, Li C, Xu H, Hu J, Zhang A, Ye S, Xu K, Dong J, “Precise Reconstruction of Veins and Bile Ducts in Rat Liver Transplantation.” Cell Biochem Biophys, 68(1): 55-65, 2014.
 
[3]  Nagai K, Yagi S, Uemoto S, Tolba RH. “Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction,” J Vis Exp, 3(73): e4376, 2013.
 
[4]  Hori T, Nguyen JH, Zhao X, Ogura Y, Hata T, Yagi S, Chen F, Baine AM, Ohashi N, Eckman CB, Herdt AR, Egawa H, Takada Y, Oike F, Sakamoto S, Kasahara M, Ogawa K, Hata K, Iida T, Yonekawa Y, Sibulesky L, Kuribayashi K, Kato T, Saito K, Wang L, Torii M, Sahara N, Kamo N, Sahara T, Yasutomi M, Uemoto S, “Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide,” World J Gastroenterol, 16(25): 3120-3132, 2010.
 
[5]  Hori T, Uemoto S, Zhao X, Chen F, Baine AT, Gardner LB, Ohashi N, Conkle F, Castanedes-Casey M, Phillips VR, Rousseau LG, Murray M, Kamo N, Nguyen JH, “Surgical guide including innovative techniques for orthotopic liver transplantation in the rat: Key techniques and pitfalls in whole and split liver grafts,” Ann Gastroenterol, 23(4): 270-295, 2010.
 
[6]  Luan X, Liu Y, Li M, “The Role of CD14 and Toll-Like Receptor 4 of Kupffer Cells in Hepatic Ischemia-Reperfusion Injury in Rats.” Transplant Proc, 44(4): 937-941, 2012.
 
[7]  Li J, Lai X, Chen Y, Niu B, Gong J, “Endotoxin Tolerance Attenuates Liver Ischemia/Reperfusion Injury by Down-Regulation of Interleukin-1 Receptor-Associated Kinase 4 in Kupffer Cells.” Transplant Proc, 43(7): 2531-2535, 2011.
 
[8]  Xue Q, Yuan Z, Chen Z, Hao R, Liu C, Tu B, “Protective Role of Nitric Oxide Induced by Ischemic Preconditioning on Cold Ischemic-Reperfusion Injury of Rat Liver Graft.” Transplant Proc, 44(4): 948-951, 2012.
 
[9]  Sun K, Chen Y, Liang SY, Liu ZJ, Liao WY, Ou ZB, Tu B, Gong JP, “Effect of taurine on IRAK4 and NF-kappa B in Kupffer cells from rat liver grafts after ischemia-reperfusion injury,” Am J Surg, 204(3): 389-395, 2012.
 
[10]  Chen G, Wang S, Bie P, Li X, Dong J, “Endogenous Bile Salts Are Associated With Bile DUCT Injury in the Rat Liver Transplantation Model.” Transplantation, 87(3): 330-339, 2009.
 
[11]  Ishii E, Shimizu A, Kuwahara N, Kanzaki G, Higo S, Kajimoto Y, Arai T, Nagasaka S, Masuda Y, Fukuda Y, “Hepatic Artery Reconstruction Prevents Ischemic Graft Injury, Inhibits Graft Rejection, and Mediates Long-term Graft Acceptance in Rat Liver Transplantation,” Transplant Proc, 45(5): 1748-1753, 2013.
 
[12]  Hori T, Gardner LB, Chen F, Baine AM, Hata T, Uemoto S, Nguyen JH, “Impact of Hepatic Arterial Reconstruction on Orthotopic Liver Transplantation in the Rat,” J Invest Surg. 25(4): 242-252, 2012.
 
[13]  Ma Y, Wang GD, Guo ZY, Guo ZG, He XS, Chen GH, “Surgical techniques of arterialized orthotopic liver transplantation in rats,” Chin Med J (Engl), 120(21): 1914-1917, 2007.