American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2017, 5(1), 25-27
DOI: 10.12691/ajmr-5-1-4
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Identification of Fungal Growth from the Internal Organs of Preserved Human Cadavers

Sambasivarao Yaragalla1, and Amruta Rajput2,

1Microbiology, Spartan Health Sciences University, Community Health and Research Centre of Spartan, St. Lucia

2Anatomy, Spartan Health Sciences University, St. Lucia, Caribbean

Pub. Date: March 29, 2017

Cite this paper:
Sambasivarao Yaragalla and Amruta Rajput. Identification of Fungal Growth from the Internal Organs of Preserved Human Cadavers. American Journal of Microbiological Research. 2017; 5(1):25-27. doi: 10.12691/ajmr-5-1-4

Abstract

Cadavers remain a principal teaching & research tool for anatomists and microbiologists. Infectious pathogens in preserved cadavers that present particular risks include Bacteria, Viruses and Prions such as Mycobacterium, hepatitis B and C, HIV and encephalopathies . It is often claimed that 10% formalin fixatives are effective in inactivation of these agents. The purpose of this study is to determine if anatomy preserved cadavers fixed in a formalin solution and internal organs are a possible source of introduction of microorganisms into the anatomy laboratory. Routinely preserved cadavers were sampled include Spinal cord, Brain and Lung. Using conventional microbiologic culture and identification methods, the research group was able to successfully recover and identify a variety of organisms from all samples includes surface and internal organs. Three different fungal species, Trichophyton spp, Aspergillus spp and Penicillium spp, were isolated from internal organs of preserved cadaver. The results indicate that preserved cadavers processed with 10% buffered formalin have viable organisms on their surfaces and internal organs that can be a source of contamination. In this brief review, we describe the infectious pathogens that can be detected in preserved internal organs from the cadavers and suggest safety guidelines including airborne precautions for the protection of all who handle and visit cadavers against infectious hazards in the anatomy lab. The results of this research support the use for further analysis to prevent infectious diseases in Anatomy lab who handle preserved cadavers and dissect internal organs.

Keywords:
Cadavers research methods contamination cadaver dissection medical curriculum gross anatomy infection mycobacterium hepatitis AIDS HIV prion

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References:

[1]  Bayramoglu A., Demiryurek D., Ustacelebi S. (2002) Infective agents in fixed human cadavers: a brief review and suggested guidelines. Anat. Rec. 269: 194-197.
 
[2]  Brown P, Gibbs CJ, Gajdusek DC, Cathala F, LaBauge R. 1986. Transmission of Creutzfeldt-Jakob disease from formalin-fixed, paraffin embedded human brain tissue. N Engl J Med 315: 1614-1615.
 
[3]  Burton Tabaac1, Geoffrey Goldberg1, Lia Alvarez1, Molly Amin1, Kathleen Shupe-Ricksecker1, Fernando Gomez2 (2012) Bacteria detected on surfaces of formalin fixed anatomy cadavers, IJAE Vo l. 118, n . 1: 1-5, 2013
 
[4]  De Craemer D. (1994). Postmortem viability of human immunodeficiency virus -implications for the teaching of anatomy. N. Engl. J. Med. 331: 1315.
 
[5]  Deniz demiryu ¨Rek, Alp bayramog lu, and s¸ Emsettin ustac¸ elebi (2002). Infective Agents in Fixed Human Cadavers: A Brief Review and Suggested Guidelines, The anatomical record (new anat.) 269:194-197, 2002.
 
[6]  Dr. Jitendra gupta1, Dr. Manish chaturvedi and Dr. Manish patil Embalmed cadavers –are they safe to handle, a study to see the microbial flora present in the
 
[7]  Sleigh JD, Timburry MC. 1998. Notes on medical bacteriology, 5th ed. Singapore: Churchill Livingstone.488 p.
 
[8]  Sterling TR, Pope DS, Bishai WR, Harrington S, Gershon RR, Chaisson RE. 2000. Transmission of mycobacterium tuberculosis from a cadaver to an embalmer. N Engl J Med 342: 246-248.
 
[9]  Tateishi J, Tashima T, Kitamato T. 1991. Practical methods for chemical inactivation of Creutzfeldt-Jakob disease pathogen. Microbiol Immunol 35: 163-166.
 
[10]  Timburry MC. 1997. Notes on medical virology. 11th ed. Hong Kong: Churchill Livingstone. 208 p.
 
[11]  Weed I, Baggenstoss A. 1951. The isolation of pathogens from tissues of embalmed human bodies. Am J Clin Pathol 21: 1114-1120.