American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2013, 1(4), 71-73
DOI: 10.12691/ajmr-1-4-2
Open AccessArticle

Modified Protocol for Yeast Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Janet W. S. Cheng1, Yit Er Tang1, Roland Jureen1, Raymond T.P. Lin1 and Jeanette W.P. Teo1,

1Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore, Republic of Singapore

Pub. Date: October 09, 2013

Cite this paper:
Janet W. S. Cheng, Yit Er Tang, Roland Jureen, Raymond T.P. Lin and Jeanette W.P. Teo. Modified Protocol for Yeast Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. American Journal of Microbiological Research. 2013; 1(4):71-73. doi: 10.12691/ajmr-1-4-2

Abstract

Advances in Matrix-assisted Laser Desorption Ionization–time of Flight Mass Spectrometry (MALDI-ToF MS) identification methods have greatly facilitated the rapid identification of yeasts. We validate the use of a shorter on-plate extraction method as well as a lower score cutoff value on the Bruker Biotyper. Overall, when the on-plate extraction method was used together with a score cutoff of > 1.7, a total of 151 of 187 (81%) Candida isolates were correctly identified. At species level, almost 100% of C. krusei and C. albicans isolates were correctly identified. We find the revised protocol to be effective for routine identification of yeasts.

Keywords:
protein extraction Candida identification scores

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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