In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City

Mohammed A. Abd Ali^1, , Sailh M. Kidem², Ali A. Fadhil¹ and Nusaybah KH.Saddam¹

¹College of Science, Misan University, Iraq

²College of Basic Education, Misan University, Iraq

Cite this paper:
Mohammed A. Abd Ali, Sailh M. Kidem, Ali A. Fadhil and Nusaybah KH.Saddam. In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City. American Journal of Microbiological Research. 2016; 4(5):159-163. doi: 10.12691/ajmr-4-5-6

Abstract

The study is aimed to evaluate the antibacterial activity of both natural Iraqi honeys (Sidr, Eucalyptus) and trade honey sample against MRSA Staphylococcus heamolyticus isolated from burned patients in alsader hospital-misan city then were analyzed between (October to December in 2015). The samples were taken in order to determine the bacterial profile and antibiotic susceptibility. The isolates of Staphylococcus heamolyticus were tested against 5 different antibiotics by a disk diffusion method 100% of the Staphylococcus heamolyticus isolates were resistant to the Optochin, Lincomycin, Ampicillin, Amphotercin, while the inhibitory effect of Amikacin antibiotic were (24mm) on MRSA Staphylococcus heamolyticus. Total antibacterial activity was evaluated by measuring the clear zone around the well. Honey samples were tested in different concentration (75%, 50 %, 25%, 10% and 100%). However, the results showed the absences of inhibitory zone to both natural and trade honeys type on MRSA Staphylococcus heamolyticus tested bacteria after dilution of(25% Sidr honey sample, 50% Eucalyptus honey sample, 50% Trade honey sample), while Sidr honey sample have more effective in 75%v/v concentration against MRSA Staphylococcus heamolyticus tested bacteria. The minimum inhibitory concentration (MIC) of the Sidr honey sample were also determined. Finally cytotoxicity evaluating toward human RBC, the results revealed the Iraqi honeys sample have not any cytotoxicity in all concentration. The good antimicrobial potency of sidr and Eucalyptus Iraqi honeys could potentially be used as therapeutic against MRSA Staphylococcus heamolyticus as an alternative to the costly antibiotics.

Keywords:
antibiotics (MRSA) Staphylococcus heamolyticus honey samples (MIC) minimum inhibitory concentration cytotoxicity

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References:

[1]	Bogdanov, S. (1997). Nature and origin of the antibacterial substances in honey, LWT-Food Sci.Technol. (30): pp 784-753.
[2]	Bogdanov, S;Jurendic, T.; Sieber.R.andGallmann, P. (2008). Honey for nutrition and health: A review, J.AM.Coll. Nutr., (27): pp 677-689.
[3]	Bilal, A.; Molan, P.; Sallal A. (1998).Antimicrobial activity of honey on selected microorganisms: A preliminary study. Biomed. Res. (India).(9):pp 51-54.
[4]	Church, D.; Elsayed, S.; Reid, O.; Winston, B.; Lindsay, R.(2006). Burn wound infection.Cli. Micobiol. Rev., 19(2): pp403-434.
[5]	Doern, G.; Tiarans, M.; Brneggemann, A. (1996). Emergence of high rate of antimicrobial resistanceamongviridans group Streptococcai in United StatesAntimicrob Agent. Chemother. (40): 891-4.
[6]	Ertek, M.; Yazge, H.;Erol, S. (2002). Demonstration of in vitro antagonism between fusidic acid and quinolones. J.Int Med Res (30):525
[7]	Foster, T. (1996). Staphylococcus. In: Barron s Medical Microbiology, Barron, S.; Teake, R.; James, D.; Susman, M.; Kennedy, C.; Singleton, M. and Schuenke, S. (eds). 4thedn., University of Texas Medical Branch, Galveston, USA.
[8]	Johan, P.; Harley, I.; Prescott, M. (2003). Laboratory Exercise in Microbiology. McGraw-Hill. USA., (484): 149-1538.
[9]	Johnson, D.; Mudge, C.; Wiggins, K.; Armstrong et al,. (2005). Randomized controlled trial of topical exit-sit application of honey (Medihoney) versus mupinocin for the prevention of cathere – associated infections in hemmodialysis patients. J. Am. Soc. Nephrol. (16): pp 1456-1462.
[10]	Huang, S.; Tang, R.; Chen, S. (2003). Coagulase-negative Staphylococcal bacteremia in critically ill children: risk factors andantimicrobial susceptibility. J. Micro-biolImmunol Infect (36): 51-5.
[11]	Kirby, W.; Bauer, A. Antibiotic Susceptibility Testing by a standardized single disk method Am. J. Clin. Pathol., 1966; 45(4): 493-496.
[12]	Lambert, P. (2005). Bacterial resistance to antibiotic: Modified target sites, Adv. Drug. Delive. Rev, 57(10): 1471-1485.
[13]	Leaper, D. J (2006). Silver Dressing: TheirRole in Wound Management. Int. Wound Care 3: 232-294.
[14]	Mayhall, C. (2004).Hospital epidemiology and infection control, 3rd.ed. Philadelphia: Lippincott. Wiliam and Wilikns: [pp.495-510].
[15]	Medeiros, A. (2012). Evaluation and Dissemination of 3-Lactamases Accelerated by Generations of 3-Lactam Antibiotics. Clin.Infect.Dis. 24(1): pp19-45.
[16]	Molan, P. (1992). The antibacterial activity of honey nature of the antibacterial activity-Bee world (73):pp5-28.
[17]	Molan, P. (1998). The limitation of the methods of identifying the floral source of honeys, Bee World, (79):59-68.
[18]	Moundoi, M.; Padila-Zakour, Q.; Worobo, R. (2001). Antimicrobial activity of honey against food pathogens and food spoilage microorganisms. New York State Agricultural Experiment Station. (1): pp61-71.
[19]	NCCLS. (2002). Performance Standards for Antimicrobial Disk Susceptibility Testing. Twelfthinformation Supplement.
[20]	Nzeako, B.; Hamdi, J. (2000). Antibacterial potential of honey on some microbial isolates, Med. Sci.2(2000) pp75-79.
[21]	O'meara S, Al-Kurdi D, Ologun V, &Ovington l. G. (2010). Antibiotics & Antiseptics for Various Leg Ulcers. Cochrane Data Base Syst.
[22]	Ouattara, B.; Simard, R. Holley, R; Piette, G.; Begin, A. (1997). Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. Int.J. Food. Microbial.(37): 155-162.
[23]	Paulus, H.;Kwakman, S. and Sebastian, A. (2012). Antibacterial components of honey. J. IUBMB.Life, 64(1):pp 48-55.
[24]	Shahid, M. (2009). Honey: Biological characteristics and potential role in disease management, cha 10:New Strategies.
[25]	Tadeg, H.; Mohammed, E.; Asres, K.; Geber-Mariam, T. (2005). Antimicrobial activities of some selected tradition Ethiopian medicinal plants used in the treatment of skin disorders. J. Ethanopharmacol., ; 43: 173-177.
[26]	Thorn R.M, Greenman J, & Austin A, (2006). An invitro Study of Antimicrobial Activity & Efficacy of Iodine Generating Hydrogel Dressing. Wound Care 15; 305-10.
[27]	Winn, W.; Allen, S.; Janda, W.; Koneman, E.; Procop, G.; Schreckenbergre, P.; Woods, G. (2006). Koneman's Color Atlas and Textbook of Diagnostic Microbiology.6th ed. Philadelphia.
[28]	Xian-gou, H. and Ursula, M. (1994). Antifungal compound from Solanumnigrescens. J. Ethano Pharmacology., (43): 173-177.