American Journal of Microbiological Research
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American Journal of Microbiological Research. 2016, 4(5), 143-146
DOI: 10.12691/ajmr-4-5-3
Open AccessArticle

Qualitative Phytochemistry and Antibacterial Resistance Pattern of Leaves and Stem Bark Extracts of Jatropha curcas

Richard Rebecca1, , Denwe D Samuel2, Yerima M.Bello3 and Olatunde K.Simeon4

1Department of Biological Science, Faculty of Science, Federal University Gashua, Yobe State, Nigeria

2Department of Biological Science, Faculty of Science, Nigerian Defence Academy, Kaduna State, Nigeria

3Department of Microbiology and Biotechnology, Faculty of Science, Federal University Dutse, Jigawa State, Nigeria

4Department of Pure and Applied Biology, Faculty of Pure and applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

Pub. Date: September 06, 2016

Cite this paper:
Richard Rebecca, Denwe D Samuel, Yerima M.Bello and Olatunde K.Simeon. Qualitative Phytochemistry and Antibacterial Resistance Pattern of Leaves and Stem Bark Extracts of Jatropha curcas. American Journal of Microbiological Research. 2016; 4(5):143-146. doi: 10.12691/ajmr-4-5-3

Abstract

Jatropha caurcas (Euphorbiaceae) is used in folklore medicine to cure various ailments in Africa, Asia and Latin America. Present study investigated the phytochemical components and antimicrobial activities of Jatropha caurcas against some selected clinical isolates. The leaf and stem bark was macerated successively in ethanol and aqueous solvent for 24 hrs each. Qualitative phytochemistry screening of aqueous and ethanolic solvent extracts of plant materials was conducted using standard methods. The antibacterial susceptibility assay was carried out on three clinical isolates by preparing discs of a standard concentration of aqueous extracts of Jatropha caurcas. Result obtained indicates the presence of some secondary metabolites which are glycosides, flavonoids, phenols, tannin, steroids, reducing sugar and terpenoids. It was observed that alkaloids and saponins were absence in aqueous extract of leaves and stem bark solvent extract but present in stem bark extracts. However, ethanolic leaves extract contains no alkaloids and saponins. Susceptibility pattern of Pseudomonas aeruginosa, Escherichia coli and Shigella dysentriae was measured to be 17mm, 23mm and 24mm respectively. Present investigation could serve as a panacea for emergence of multidrug resistance bacteria and also, the analysis of the secondary metabolites will provide the pharmacognosy and drug development sector preliminary information about the phytochemical constituent of Jatropha caurcas (Euphorbiaceae).

Keywords:
Jatropha caurcas phytochemical antibacterial susceptibility extracts

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References:

[1]  Namuli A, N.; Abdullah,C.C.; Sieo, S.W.; Zuhainis and E. Oskoueian. (2011). Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. Extracts. Journal of Medicinal Plants Research Vol. 5(16), pp. 3982-3990.
 
[2]  Ayoola P.B., Onawumi O.O and Faboya O.O.(2011). Chemical evaluation and nutritive values of Tetracarpidium conophorum (Nigeria walnut) seeds. Journal of pharmaceutical and biomedical sciences. 11 (5):320-342.
 
[3]  De-F├ítima A.; Modolo LV.; Conegero LS.; Pilli RA.; Ferreira CV.; Kohn LK and de-Carvalho JE (2006). Lactones and their derivatives: biological activities, mechanisms of action and potential leads for drug design. Curr. Med. Chem. 13: 3371-3384.
 
[4]  El Diwani, G.; El Rafie, S. and Hawash, S. (2009). Antioxidant activity of extracts obtained from residues of nodes, leaves, stem and root of Egyptian Jatropha curcas. African Journal of Pharmacy and Pharmacology, 3(11): 521-530.
 
[5]  Gbolade, A. A. (2009). Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. Journal of Ethnopharmacology, 121: 135-139.
 
[6]  Harborne, J. B. (1973). Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall Ltd, London.; Pp. 279.
 
[7]  Khurram M.; Hameed A and Khan M A, (2012) Antibacterial potentials of quercus baloot griff. Journal of Medicinal Plants Research, 2012; 6: 1244-1249.
 
[8]  Pundir, R.K.; Jain, P and Sharma, C. (2010) Antimicrobial activity of ethanolic extracts of syzygium aromaticum and Allium sativum against food associated bacteria and fungi Ethnobotanical leaflets 14; 344-60.
 
[9]  Igbinosa O.O. and Igbinosa E.O. (2009). Antimicrobial activity and phytochemical screening of stem bark extract of Jatropha curcas. African Journal of Pharmacy and Pharmacology, 3(2): 055-062.
 
[10]  James, O,; Unekwojo, E.G. and Ojochenemi, A. A. (2011). Assessment of Biological Activities: A Comparison of Pergularia daemia and Jatropha curcas Leaf Extracts. British Biotechnology Journal, 1(3):85-100.
 
[11]  Kalimuthu, K,; Vijayakumar, S. and Senthilkumar, R. (2010). Antimicrobial activity of the biodiesel plant, Jatropha curcas L. International Journal of Pharmacy and Biological Sciences, 1(3): 1-5.
 
[12]  Oseni, L. A. and Alphonse, P. K. (2011). Comparison of antibacterial properties of solvent extracts of different parts of Jatropha curcas (Linn). International Journal of Pharmaceutical and Phytopharmacological Research, 1(3): 117-123.
 
[13]  Oskoueian, E,; Abdullah, N,; Saad, W. Z,; Omar, A,; Ahmad, S,; Kuan, W. B,; Zolkifli, N. A,; Hendra, R. and Ho, Y. W. (2011). Antioxidant, anti inflammatory and anticancer activities of methanolic extracts from Jatropha curcas Linn. Journal of Medicinal Plants Research, 5(1): 49-57.
 
[14]  Rathee, P,; Chaudhary, H,; Rathee, S,; Rathee, D,; Kumar, V. and Kohli, K. (2009). Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflammation and Allergy Drug Targets, 8(3): 229-235.
 
[15]  Shahidi F (2000). Antioxidant factors in plant foods and selected oilseeds. BioFactors, 13:179-185.
 
[16]  Shahidi F,; McDonald J,; Chandrasekara A and Zhong Y (2008). Phytochemicals of foods, beverages and fruit vinegars: chemistry and health effects. Asia Pacific journal Clinical Nutrition., 17: 380-382.
 
[17]  Sofowora, E. A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. Pp 289.
 
[18]  Thomas, R,; Sah, N. and Sharma, P. (2008). Therapeutic Biology of Jatropha curcas: A Mini Review. Current Pharmaceutical Biotechnology, 9(4): 315-324.
 
[19]  Wini, S.P,; Osman, M,; Emmanuel, D.S. and Sreedevi, T.K. (2006). Improved livelihoods And environmental protection through biodiesel plantations in Asia. Asian Biotechnology and Development Review, 3 (2): 11-29.
 
[20]  Sarin S,; Nimit S,; Tawatchai S and Dawan S. (2010). Antibacterial activities of crude extracts from physic nut (Jatropha curcas) seed residues. Science Asia 36:346-348
 
[21]  Kalimuthu K,; Vijayakumar S and Senthilkumar R. (2010). Antimicrobial Activity Of The Biodiesel Plant, Jatropha Curcas L. International Journal of Pharma and Bio Sciences. 1(3):1-5
 
[22]  Ekundayo F. O,; Adeboye C. A. and Ekundayo E. A. (2011). Antimicrobial activities and phytochemical screening of pignut (Jatrophas curcas Linn.) on some pathogenic bacteria. Journal of Medicinal Plants Research Vol. 5(7), pp. 1261-1264.
 
[23]  Obiukwu, C.E and Nwanckwu, K.E (2010) In vitro antibacterial studies of five medicinal plants as alternative therapy for methicillin-resistant Staphylococcus aureus (MRSA) infection, International Science Research Journal 2:66-69.
 
[24]  Wakirwa, J.H. Ibrahim, P., Madu, S.J., (2013). Phytochemical screening and in vitro antimicrobial analysis of the ethanol stem bark extract of Jatropha curcas Linn. (Euphorbiaceae). Int. Res J. Pharm. 4(3): 97-100.
 
[25]  Haslam, E., Lilley, T. H., Martin, Y and Magnolotto, R. (1989). Traditional Herbal Medicine; The Roles of Polyphenols, Plants Medica: 4(2):1-8.
 
[26]  Obasi, L.N., Madus, P., Ejikeme, A. and Cemaluk, A.C. (2011). Antimicrobial and phytochemical activity of methanolic extract and its fractions of Jatropha curcas linn stem bark. African Journal of Pure and Applied Chemistry. 5(5): 91-96.
 
[27]  Kumar, A. R., Subburathinam, K. M. and Prabakar, G. (2007). Phytochemical screening of selected medicinal plants of asclepiadaceae family. Asian Journal of Microbiology and Biotechnology Environmental Science 9(1): 177-180.