American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2016, 4(3), 85-89
DOI: 10.12691/ajmr-4-3-4
Open AccessArticle

Isolation and Molecular Identification of Fungi in Stored Maize (Zea mays L) and Groundnuts (Arachis hypogaea L) in Ngaoundere, Cameroon

Agwanande Ambindei Wilson1, , Tatsadjieu Ngoune Leopold2, Jazet Dongmo Pierre Michel3, Priya P4, Anie Mariya4, Manilal V. B.4, Krishnakumar B4 and Amvam Zollo Paul Henri5

1ENSAI, University of Ngaoundere, P.O. box 454, Ngaoundere, Cameroon

2IUT, University of Ngaoundere, P.O. box 454, Ngaoundere, Cameroon

3Faculty of Science, University of Douala, P.O. box 24157, Douala, Cameroon

4Environmental Technology Division, CSIR-NIIST, 695019 Thiruvananthapuram, India

5Faculty of Science, University of Ngaoundere, P.O. box 454, Ngaoundere, Cameroon

Pub. Date: June 17, 2016

Cite this paper:
Agwanande Ambindei Wilson, Tatsadjieu Ngoune Leopold, Jazet Dongmo Pierre Michel, Priya P, Anie Mariya, Manilal V. B., Krishnakumar B and Amvam Zollo Paul Henri. Isolation and Molecular Identification of Fungi in Stored Maize (Zea mays L) and Groundnuts (Arachis hypogaea L) in Ngaoundere, Cameroon. American Journal of Microbiological Research. 2016; 4(3):85-89. doi: 10.12691/ajmr-4-3-4

Abstract

A knowledge of the specific fungi responsible for food spoilage in an ecological zone is paramount for proper preservation. The main objective of this study was to isolate and identify at the molecular level the different fungal species from contaminated stored maize and groundnuts. Contaminated grains from local markets in Ngaoundere, Cameroon were used for the study. Their average percentage water content was 16.75±0.27 and 11.24±0.31 for maize and groundnuts respectively. Nine fungal strains were isolated and identified through 18S rDNA sequencing. It was found that Rhizopus oryzae, Aspergillus flavus, Aspergillus oryzae and Cunninghamella polymorpha were common in both maize and groundnuts. Aspergillus tamari, Talaromyces purpureogenus and Penicillium citrinum were present only in maize, while Aspergillus parasiticus and Rhizopus stolonifer were identified only from groundnuts. The novel information presented in this study will help to formulate measures to control the fungal contamination of stored maize and groundnut.

Keywords:
stored grains Fungal DNA Aspergillus Rhizopus Penicillium and Cunninghamella

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References:

[1]  Pitt, J. I., and Hocking, A. D., Fungi and Food Spoilage, 3rd edition, Springer Science plus Business Media, LLC, 233 Spring Street, New York, NY 10013, USA, 524 pp. (2009).
 
[2]  Meyer A., Deiana J. and Bernard A. Cour de microbiologie générale. Doin. France, unpublished data. (2004).
 
[3]  FAO, Safety evaluation of certain mycotoxins in food. Prepared by the 56th meeting of the joint FAO/WHO Expert Committee on Food Additives (JECFA). FAO food and nutrition paper, Rome, FAO 74. 1-25 (2001).
 
[4]  Golob, P., Kutukwa, N., Devereau, A., Bartosik, R. E. and Rodriguez, J. C., Chapter two: Maize. In Crop Post-Harvest: Science and Technology, Volume 2. R. Hodges, and G. Farrell, eds. Ames, Iowa. Blackwell Publishing Ltd. (2004).
 
[5]  Brewbaker, J. L. “Corn production in the tropics: The Hawaii experience”, College of tropical agriculture and human resources University of Hawaii at Manoa. (2003), [http://www.ctahr.hawaii.edu/oc/freepubs/pdf/corn2003.pdf], (Accessed October 2012).
 
[6]  Tilahun, S. “Grain based Ethiopian traditional common foods processing science and technology”. In Training module for center of research on grain quality, processing and technology transfer, Haramaya University, Ethiopia. (2007).
 
[7]  Egal, S., Hounsa, A., Gong, Y. Y., Turner, P. C, Wild, C. P., Hall, A. J., Hell, K. & Cardwell, K. F., “Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa”, International Journal of Food Microbiology 104(2): 215-224. (2005).
 
[8]  AOAC International, Official Methods of Analysis 17th Ed., Gaithersburg, MD, Method 925.10 (2000).
 
[9]  Hocking, A.D., Pitt, J.I., Samson, R.A. and Thrane, U. (eds). Advances in Food Mycology. Springer Science+Business Media, Inc., 233 Spring Street, New York, NY 10013, USA. 375 pp. 2006a.
 
[10]  Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Heled J, Kearse M, Moir R, Stones-Havas S, Sturrock S, Thierer T and Wilson A (2010) Geneious v5.1, Available from http://www.geneious.com.
 
[11]  Highley E., Wright E.J., Banks H. J. and Champ B.R., ed. (1994). “Stored Product Protection” in Proceedings of the 6th International Working Conference on Stored-product Protection, CAB international, Canberra, volume 2, pages 969-1083.
 
[12]  Walker G. M. and White N. A. Introduction to Fungal Physiology in Fungi Biology and Applications by Kevin Kavanagh (Ed). John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England. (2005).
 
[13]  White, T. J., T. Bruns, S. Lee, and J. W. Taylor Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, M. A., D. H. Gelfand, J. J. Sninsky, and T. J. White. Academic Press, Inc., New York. (1990).
 
[14]  Njobeh, P. B., Dutton, M. F., Koch, S. H., Chuturgoon, A., Stoev, S. and Seifert, K., “Contamination with storage fungi of human food from Cameroon”, International Journal of Food Microbiology 135, 193-198. (2009).
 
[15]  Mukvold, G.P. and Desjardins A. E., “Fumonisins in maize: can we reduce their occurrence?” Plant Disease 81: 556-565. (1997).