American Journal of Microbiological Research
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American Journal of Microbiological Research. 2016, 4(3), 77-80
DOI: 10.12691/ajmr-4-3-2
Open AccessArticle

Effect of Trigona Honey to mRNA Expression of Interleukin-6 on Salmonella Typhi Induced of BALB/c Mice

Yuliana Syam1, Rosdiana Natsir2, Sutji Pratiwi Rahardjo3, Andi Nilawati Usman4, Ressy Dwiyanti5 and Mochammad Hatta6,

1School of Nursing, Hasanuddin University, Makassar, Indonesia

2Department of Biochemistry, Hasanuddin University, Makassar, Indonesia

3Department of ENT, Hasanuddin University, Makassar, Indonesia

4Department of Epidemiology, Indonesia Timur University; Daya Regional Hospital-College of Health Science, Makassar, Indonesia

5Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

6Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Pub. Date: May 14, 2016

Cite this paper:
Yuliana Syam, Rosdiana Natsir, Sutji Pratiwi Rahardjo, Andi Nilawati Usman, Ressy Dwiyanti and Mochammad Hatta. Effect of Trigona Honey to mRNA Expression of Interleukin-6 on Salmonella Typhi Induced of BALB/c Mice. American Journal of Microbiological Research. 2016; 4(3):77-80. doi: 10.12691/ajmr-4-3-2


Weak inflammatory response after Salmonella infection can cause persistent infection and facilitate the long survival of pathogens. Honey can induce key immunomodulators such as TNF-α, interleukin-6 (IL-6) and IL-1, that it can be used in the treatment of bacterial infectious diseases caused by Salmonella typhi. The purpose of this study is to determine the effect of honey on the mRNA expression of IL-6 in Salmonella enterica Typhi induced of BABL/c mice. The study used experimental pretest-posttest control design. Honey treatment was given for 7 days commencing after the induction of Salmonella bacteria. 20 BABL/c males mice whose weight 25-29 grams, were divided into four groups where 5 mice per group within; the negative control group was given regular feed without bacteria induction, the positive control group was given regular feed with bacteria induction, 0.27 ml/kg-weight honey group and 0.27 ml/kg-weight of Propolis honey group. Blood samples for examination of mRNA expression was examined three times that prior to the induction, 24 hours after induction and 72 hours after induction of Salmonella. The results showed that 0.27 ml/kg-weight of Propolis honey group showed the highest mRNA expression (p = 0.000) for both after 24 hours after induction of Salmonella typhi (p = 0.000) and 72 hours after induction of Salmonella typhi (p = 0.000). We conclude that there was effect of honey on the mRNA IL-6 expression in Salmonella typhi induced of BALB/c mice.

Interleukin-6 Honey Salmonella typhi Balc/c mice

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