Molecular Screening of Staphylococcal Enterotoxin Type A Encoding Gene from MRS Clinical Isolates

H. A. Hussein^1, , M. K. Ibrahim², H. A. Nour El-Din³, E. H. El-Shatoury², M. E. El-Fouly¹, S. S. Abd-Eal¹ and M. N. Abu El-Naga¹

¹National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo 11787, Egypt

²Faculty of Science, Ain Shams University

³Agricultural Genetic Engineering Research Institute (AGERI)

Cite this paper:
H. A. Hussein, M. K. Ibrahim, H. A. Nour El-Din, E. H. El-Shatoury, M. E. El-Fouly, S. S. Abd-Eal and M. N. Abu El-Naga. Molecular Screening of Staphylococcal Enterotoxin Type A Encoding Gene from MRS Clinical Isolates. American Journal of Microbiological Research. 2016; 4(2):68-72. doi: 10.12691/ajmr-4-2-5

Abstract

Antibiotic resistance is a great problem in Egyptian hospitals and clinical centers. The aim of this study was the characterize of different clinical isolates of antibiotic resistant especially methicillin resistant staphylococci (MRS) on the basis of their production of enterotoxin A. Eighty four samples of bacteria were isolated from wound pus, throat, sputum, conjunctiva, urine and stool of patients in the Egyptian National Centre for Clinical and Environmental Toxicology. Sixty-two isolates had the typical biochemical characteristics of the Staphylococcus aureus, while two isolates were identified as Staphylococcus epidermidis. Forty two of the isolates were methicillin resistant, all were showed multidrug resistant to thirteen antibiotic. Twenty isolates encoded ent A gene (nineteen were Staphylococcus aureus and one was Staphylococcus epidermidis). Only twelve out of twenty ent A gene carriers can produce ent A protein which detected by enzyme linked immunosorbent assay (ELISA). Also, all carried mec A gene isolates were resistant to vancomycin. These findings prove that the staphylococcal clinical infections can cause food borne illness and vice versa.

Keywords:
MRSA MRS Vancomycin Resistance Ent A gene Staphylococcus aureus

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