American Journal of Microbiological Research
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American Journal of Microbiological Research. 2015, 3(4), 144-147
DOI: 10.12691/ajmr-3-4-4
Open AccessArticle

Screening of MRSA in and outside Benghazi Hospitals

Saleh. H. Baiu1 and Nadia. E. AL-Abdli2,

1Department of Botany, Faculty of Science, Benghazi University, Libya

2Department of Laboratory, Eye Hospital, Benghazi, Libya

Pub. Date: August 05, 2015

Cite this paper:
Saleh. H. Baiu and Nadia. E. AL-Abdli. Screening of MRSA in and outside Benghazi Hospitals. American Journal of Microbiological Research. 2015; 3(4):144-147. doi: 10.12691/ajmr-3-4-4

Abstract

Background and Purpose: Data on the carriage rate and antibiotic sensitivity pattern of Staphylococcus aureus strains prevalent in the community are not available for many developing countries including Libya. To estimate the extent of community S. aureus transmission, in particular methicillin-resistant S. aureus (MRSA), the prevalence of S. aureus nasal colonization in a population of healthy adults was determined. Factors associated with S. aureus nasal carriage and antibiotic sensitivity patterns of the isolates were also analyzed. Methods: A cross-sectional study involving 643 adults was conducted. Nasal swabs were examined for the presence of MRSA. Epidemiological information concerning risk factors for nasal carriage was also obtained. Antibiotic susceptibility testing was performed using the disk diffusion method according to the National Committee for Clinical Laboratory Standards guidelines. MRSA strains isolated were further subjected to Automated BD Phoenix. Results: Screening for suspected carriers of MRSA showed that most of the healthcare workers (technicians, nurses, and doctors) were asymptomatically MRSA-positive. S. aureus isolates were confirmed by various biochemical tests as per latest CLSI guidelines. Cefoxitin Disk Diffusion test was performed for the detection of methicillin resistance and antibiotic susceptibility was performed against different antibiotics as per CLSI guidelines. Statistical analyses showed that there was no significant relation between MRSA carriage and age and sex of the study population. The prevalence of MRSA nasal carriage was (21.4%) among HCWs, with some differences between hospitals, also prevalence of MRSA nasal carriage was (9.6%) among community. Hospital acquired MRSA were more susceptible to various antibiotics as compared to Community acquired MRSA. Conclusion: MRSA nasal colonization was found to be low outside of the health care environment.

Keywords:
Staphylococcus aureus MRSA health care workers community

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References:

[1]  Damon,T; Arnold, M.D.; Director, M.P.H. (2008): MRSA in Illinois Descriptive Analysis of Hospital Discharge Data 2002-2006. Illinois Department of Public Health 2008.
 
[2]  Klevens, R.M; Morrison, M.A; Fridkin, S.K; Reingold, A; Petit, S; Gershman, K. (2007) Invasive methicillin-resistant Staphylococcus aureus infections in the United States. The Journal of the American Medical Association. 298 (15):1763-1771.
 
[3]  Ahmed, M. O; Abuzweda, A.R; Alghazali, M. H; El ramalli, A.K; Amri, S.G; Aghila,E.Sh; and Abouzeed, Y.M.(2010a). Misidentification of methicillin resistant Staphylococcus aureus (MRSA) in hospitals in Tripoli, Libya. Citation: Libyan J Med. 5: 5230.
 
[4]  Bassetti, M; Trecarichi, E.M; Mesini, A; Spanu, T; Giacobbe, D.R; Rossi, M; Shenone, E; Pascale, G.D; Molinari, M.P. (2011). Risk factors and mortality of healthcare-associated and community-acquired Staphylococcus aureus bacteraemia. Clin. Microbiol. Infect. 18(9):862-869.
 
[5]  Changchien, C.H; Chen ,Y.Y; Chen, S.W; Chen ,W.L; Tsay, J.G; Chu, C .(2011). Retrospective study of necrotizing fasciitis and characterization of its associated methicillin resistant Staphylococcus aureus in Taiwan. BMC Infect. Dis. 11:297.
 
[6]  Burdette, S.D; Watkins, R.R; Wong, K.K; Mathew, S.D; Martin, D.J; Markert, R.J. (2012). Staphylococcus aureus pyomyositis compared with non- Staphylococcus aureus pyomyositis. J Infect. 64:507-512.
 
[7]  Vainio, A. (2012). Molecular Methods for the Epidemiological Analysis of Methicillin- Resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae. National Institute for Health and Welfare (THL), Research 71, 164 pages. Tampere, Finland.
 
[8]  Lowy, F.D.(1998). Staphylococcus aureus infections. N Engl J Med; 339:520-32.
 
[9]  Smith, T.L; Pearson, M.L; Wilcox, K.R; Cruz, C; Lancaster, M.V; Robinson-Dunn, B. (1999). Emergence of vancomycin resistance in Staphylococcus aureus. N Engl J Med; 340: 493-501.
 
[10]  Kaplan, S.L. (2005). Implications of methicillin-resistant Staphylococcus aureus as a community acquired pathogen in pediatric patients. Infect Dis Clin North Am; 19: 747-57.
 
[11]  Wilson, J.A; Loveday, H.P; Hoffman, P.N. and Pratt, R.J. (2007). Uniform: an evidence review of the microbiological significance of uniforms and uniform policy in the prevention and control of healthcare-associated infections. Report to the Department of Health (England). J. Hosp. Infect. 66(4):301-307.
 
[12]  Al-Abdli, N.E; Baiu, S.H.(2014). Nasal Carriage of Staphylococcus in Health Care Workers in Benghazi Hospitals. American Journal of Microbiological Research, 2 (4): 110-112.
 
[13]  Cespedes, C; Miller, M; Quagliarello, B; Vavagiakis, P; Klein, R.S; and Lowy, F.D. (2002). Differences between Staphylococcus aureus Isolates from Medical and Nonmedical Hospital Personnel. J. Clin. Microbiol. 40:2594-2597.
 
[14]  Luzar, M.A; Coles, G.A; Faller, B; Slingeneyer, A; Dah, G.D; Briat, C; Wone, C; Knefati,Y; Kessler, M; and Peluso, M (1990). Staphylococcus aureus nasal carriage and infection in patients on continuous, ambulatory peritoneal dialysis. N. Engl. J Med., 322: 505-509.
 
[15]  Cheesbrough, M, District Laboratory Practice in Tropical Countries. Cambridge university, UK, 2009, 2 (2): (65-67).
 
[16]  Bauer, A. W; Kirby, W. M; Sherris, J. C. and Turck, M, Antibiotic susceptibility testing by a standard single disk method. Am. J. Clin. Pathol, 1966; 45: 493-496.
 
[17]  Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing, Twenty-second Informational Supplement. CLSI document M100-S22. Wayne. PA, 2012.
 
[18]  Deal, M; Votta, M; Halvis, S; Turng, B; Wiles, T; Reubenbd, J. (2002). Diagnostic Systems 2002. Detection of glycopeptide intermediate or Staphylococcus aureus strains by using BD phoenix Automated. 87-Microbiology system BD Diagnostic Systems. 7 Loveton Circle. Sparks, MD, USA 21152.
 
[19]  Sydnor, R.M; Perl, T.M. (2011). Hospital Epidemiology and Infection Control in Acute-Care Settings. J Hosp Infect 77: 285-289.
 
[20]  Hawkins, G; Stewart, S; Blatchford, O; Reilly, J. (2011). Should healthcare workers be screened routinely for meticillin-resistant Staphylococcus aureus? A review of the evidence. Clin Microbiol Rev 24: 141-173.
 
[21]  Ghasemian, R; Najafi, N; Shojaei Far, A.(2004). Prevalence of Staphylococcus aureus carriages among Razi health care workers of Ghaemshahr, Iran. Mazandaran Uni Med J. 44:79–85.
 
[22]  Cesur, S; Cokça, F. (2004). Nasal carriage of methicillin-resistant Staphylococcus aureus among hospital staff and outpatients. Infect ion Control and Hospital Epidemiology. 25(2):169-71.
 
[23]  Fadheel, Z.H; Perry, H.E; Henderson, R.A.(2008). Comparison of methicillin-resistant Staphylococcus aureus (MRSA) carriage rate in the general population with the health worker population. NZ J Med Lab Science 62: 4-6.
 
[24]  Mohammad Bagher, K; Mohammad Kazem, S.Y. (2009). Nasal Colonization rate of Staphylococcus aureus strains among Health Care Service Employee’s of Teaching University Hospitals in Yazd. Acta Medica Iranica 47(4):315-317.
 
[25]  Johnston, C.P; Stokes, A.K; Ross, T; Cai, M; Carroll, K.C; Cosgrove, S.E. (2007). Staphylococcus aureus colonization among healthcare workers at a tertiary care hospital. Infection Control and Hospital Epidemiology. 28:1404-1407.
 
[26]  Mathanraj, S ; Sujatha, S; Sivasangeetha, K; Parija, S.C. (2009). Screening for methicillin-resistant Staphylococcus aureus carriers among patients and health care workers of a tertiary care hospital in south India. Indian Journal of Medical Microbiology. 27:62-64.
 
[27]  Zorgani, A; Elahmer,O; Franka,E; Grera, A; Abudher, A; Ghengheah, K.S.( 2009). Detection of meticillin-resistant Staphylococcus aureus among healthcare workers in Libyan hospitals. Journal of Hospital Infection. 73:91-92.
 
[28]  Akhtar, N.(2010). Staphylococcal Nasal Carriage of Health Care Workers. Journal of the College of Physicians and Surgeons Pakistan. 20 (7): 439-443.
 
[29]  Yazgi, H; Ertek, M; Ozbek, A; Kadanali, A. (2003). Nasal carriage of Staphylococcus aureus in hospital personnel and the normal population and antibiotic resistance of the isolates. Mikrobiyol Bul . 37(2-3):137.
 
[30]  Mansour, M.K; Abd Rahman, S.A.(2006).Colonization By Methicillin Resistant Staphylococcus aureus Among Health Care Workers In Intensive Care Units. Egyptian Journal of Medical Microbiology. 15(3): 531-539.
 
[31]  Shakya, B; Shrestha, S; Mitra, T.( 2010) : Nasal carriage rate of methicillin resistant Staphylococcus aureus among at National Medical College Teaching Hospital, Birgunj. Nepal Nepal Med Coll J. 12(1):26-29.
 
[32]  Shibabaw, A; Abebe, T; Mihret, A (2013). Nasal carriage rate of methicillin resistant Staphylococcus aureus among Dessie Referral Hospital Health Care Workers; Dessie, Northeast Ethiopia. Antimicrobial Resistance and Infection Control. 2:25.
 
[33]  Truong, H; Shah, S.S; Ludmir, J; Twananana, E.O; Bafana, M; Wood S.M; Moffat, H; Steenhoff, A.P.( 2011). Staphylococcus aureus skin and soft tissue infections at a tertiary hospital in Botswana. S Afr Med J 101(6):413-416.
 
[34]  Fadeyi, A; Bolaji, B.O; Oyedepo, O.F(2010). Methicillin Resistant Staphylococcus aureus Carriage amongst Healthcare Workers of the Critical Care Units in a Nigerian Hospital. Am J Infect Dis . 6 (1) :18-23.
 
[35]  Elgadi, S.A. (2000). Carriage rate and antibiotic sensitivity of Staphylococcus aureus in & outside Children's hospital-Benghazi Libya. An M.Sc, Gar younis University, Faculty of medicine.
 
[36]  Opal, S.M; Mayer, K.H; Stenberg, M.J.(1990). Frequent acquisition of multiple strains of methicillin- resistant Staphylococcus aureus by healthcare workers in an endemic hospital environment. Infect Control Hosp Epidemiol. 11:479-485.