American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2015, 3(4), 129-134
DOI: 10.12691/ajmr-3-4-2
Open AccessArticle

Immunomodulator Potential of Miana Leaves(Coleus scutellarioides (L) Benth) in Prevention of Tuberculosis Infection

Sesilia Rante Pakadang1, Chatarina Umbul Wahjuni2, Hari Basuki Notobroto2, Dwi Winarni3, Ressy Dwiyanti4, 5, Yadi6, 5, Muhammad Sabir4, 5 and Mochammad Hatta5,

1Health Technology Department, Ministry of Health, Makasssar, Indonesia

2Faculty of Public Health, Airlangga University, Surabaya, Indonesia

3Faculty of Life Sciences and Technology, Airlangga University, Surabaya, Indonesia

4Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

5Molecular Biology and Immunology Laboratory for Infectious Diseases, Microbiology Department, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

6Department of Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Pub. Date: July 08, 2015

Cite this paper:
Sesilia Rante Pakadang, Chatarina Umbul Wahjuni, Hari Basuki Notobroto, Dwi Winarni, Ressy Dwiyanti, Yadi, Muhammad Sabir and Mochammad Hatta. Immunomodulator Potential of Miana Leaves(Coleus scutellarioides (L) Benth) in Prevention of Tuberculosis Infection. American Journal of Microbiological Research. 2015; 3(4):129-134. doi: 10.12691/ajmr-3-4-2

Abstract

Aim, The aim of this study to investigate the immunonomodulator effects of miana leaves (Coleus scutellarioides (L) Benth) on prevention of tuberculosis in wistar rats, Method, Samples of white male Wistar rats were divided into 4 groups, the samples were treated with miana leaves extract (EDM) and then infected by intra tracheal M. tuberculosis H37Rv strain and subsequently given a placebo, EDM and GAB (combined Rifampicin and EDM) and healthy control animals were treated with EDM. In this study we measure the level of Immunomodulatory parameter; the number of T-lymphocytes and CD4 T-cells measured by flowcytometry method, the levels of IFN-γ and TNF-α were measured by ELISA and the number of M. tuberculosis colonies derived from the rat lung in LJ media. Result, Results showed the increasing of T-lymphocytes, CD4 T-Cells, the levels of IFN-γ, TNF-α and decreasing in the number of M. tuberculosis colonies after EDM treatment. Conclusion, Miana leaves extract (Coleus scutellarioides, (L) Benth.) increased the number of T-lymphocytes, CD4 T-cell counts, levels of IFN-γ and TNF-α and decreased in the number of M. tuberculosis colony in infected wistar lung.

Keywords:
immunomodulator miana leaves (Coleus scutellarioides (L) Benth) mycobacterium tuberculosis CD4 IFN-γ TNF-α

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