American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: Editor-in-chief: Apply for this position
Open Access
Journal Browser
American Journal of Microbiological Research. 2015, 3(4), 129-134
DOI: 10.12691/ajmr-3-4-2
Open AccessArticle

Immunomodulator Potential of Miana Leaves(Coleus scutellarioides (L) Benth) in Prevention of Tuberculosis Infection

Sesilia Rante Pakadang1, Chatarina Umbul Wahjuni2, Hari Basuki Notobroto2, Dwi Winarni3, Ressy Dwiyanti4, 5, Yadi6, 5, Muhammad Sabir4, 5 and Mochammad Hatta5,

1Health Technology Department, Ministry of Health, Makasssar, Indonesia

2Faculty of Public Health, Airlangga University, Surabaya, Indonesia

3Faculty of Life Sciences and Technology, Airlangga University, Surabaya, Indonesia

4Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

5Molecular Biology and Immunology Laboratory for Infectious Diseases, Microbiology Department, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

6Department of Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Pub. Date: July 08, 2015

Cite this paper:
Sesilia Rante Pakadang, Chatarina Umbul Wahjuni, Hari Basuki Notobroto, Dwi Winarni, Ressy Dwiyanti, Yadi, Muhammad Sabir and Mochammad Hatta. Immunomodulator Potential of Miana Leaves(Coleus scutellarioides (L) Benth) in Prevention of Tuberculosis Infection. American Journal of Microbiological Research. 2015; 3(4):129-134. doi: 10.12691/ajmr-3-4-2


Aim, The aim of this study to investigate the immunonomodulator effects of miana leaves (Coleus scutellarioides (L) Benth) on prevention of tuberculosis in wistar rats, Method, Samples of white male Wistar rats were divided into 4 groups, the samples were treated with miana leaves extract (EDM) and then infected by intra tracheal M. tuberculosis H37Rv strain and subsequently given a placebo, EDM and GAB (combined Rifampicin and EDM) and healthy control animals were treated with EDM. In this study we measure the level of Immunomodulatory parameter; the number of T-lymphocytes and CD4 T-cells measured by flowcytometry method, the levels of IFN-γ and TNF-α were measured by ELISA and the number of M. tuberculosis colonies derived from the rat lung in LJ media. Result, Results showed the increasing of T-lymphocytes, CD4 T-Cells, the levels of IFN-γ, TNF-α and decreasing in the number of M. tuberculosis colonies after EDM treatment. Conclusion, Miana leaves extract (Coleus scutellarioides, (L) Benth.) increased the number of T-lymphocytes, CD4 T-cell counts, levels of IFN-γ and TNF-α and decreased in the number of M. tuberculosis colony in infected wistar lung.

immunomodulator miana leaves (Coleus scutellarioides (L) Benth) mycobacterium tuberculosis CD4 IFN-γ TNF-α

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Summary of Health profile of South Sulawesi Province 2012. Makassar: Provincial Health Office of South Sulawesi; 2012.
[2]  (WHO) WHO: Global tuberculosis report 2014. In. Geneva: WHO; 2014.
[3]  Bannister B, Gillespie S, Jones J: Infection Microbiology and Management. Victoria Australia.: Blackwell Publishing Asia Pty Ltd, 2006.
[4]  Robbins C: Pocket companion to Robbins & Cotran Pathologic Basis of Disease,: Elsevier Inc.; 2009.
[5]  Raja A: Immunology of tuberculosis, Review article. Indian J Med Res 2004 120:213-232.
[6]  RA. F, Perez TM., Aguilar LD., Rangel MJ., Garcia IE., Pando RH., Parra SE.: Transfer factors as immunotherapy and supplement of chemotherapy in experimental pulmonary tuberculosis. Clinical and Experimental Immunology 2004, 136(2):215-223.
[7]  van Zutphen LFM, Baumans V, Beynen AC: Principles of Laboratory Animal Science. In: A contribute to the humane use and care of animals and to the quality of experimental results. edn. Amsterdam, Netherlands.: Elsevier Science Publishers BV; 1993: 189-270.
[8]  Leon DA., Zumarraga MJ., Oopeza RJ., Gioffre AK., Bernardelli A., Estevez HO., Cataldi AA., Pando RH.: Mycobacterium bovis with different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis. Clinical and Experimental Immunology (2009), 157(1):139-147.
[9]  Arifa M: Mekanisme ekstrak etanol herba Centela asiatica (pegagan) dalam meningkatkan apoptosis sel alveolar makrofag dari jaringan paru tikus yang diinfeksi Mycobacterium tuberculosis,. Surabaya: Universitas Airlangga; 2012.
[10]  Smith J.B., Mangkoewidjojo S.: The Care Breeding and Management of Experimental Animals for Research in the Tropics. International Development Program of Australian Universities and Colleges Limited (IDP) 1987:36-38.
[11]  Singhal A., Aliouat EM, Herve´ M: Experimental Tuberculosis in the Wistar Rat: A Model for Protective Immunity and Control of Infection. PloS one 2011, 6(4):18632.
[12]  Espinosa DAM., Rodriguez VM., Leon DA., Rosales R., Casillas FC., Pando RH.: Therapeutic Effect of Recombinant Adenovirus Encoding Interferon-γ in a Murine Model of progressive Pulmonary Tuberculosis. Molecular Therapy 2008, 16(6):1065-1072.
[13]  Hidayat S, Wahyuni S, Andalusia S: Seri Tumbuhan Obat Berpotensi Hias,. Jakarta: PT. Elex Media Komputindo.; 2008.
[14]  Venkatesha S, Rajaiah R, Berman B: Immunomodulation of Autoimmune Arthritis by Herbal CAM. Evidence Based Complementary and Alternative Medicine; 2011.
[15]  Cooper A.M., Mayer-Barber K.D., Sher A.: Role of Innate Cytokines in Mycobacterial Infection, Review. Mucosal Immunology 2011, 4:232-260.
[16]  Curina G., Paternesi B., Cagiota M., de Gioseppe AM, Forti K., Mazzone P., Pasquali P.: Cellular immune response of mice experimentally infected with Mycobacterium bovis BCG. Oxford Journals; Life Sciences ; Intimm Meeting Abstracts 2010, 22(Suppl 1 Pt 3.):061-063.
[17]  Alvares: Immunoregulatory from IFN-γ,. In. Edited by Department of Biology DC. Davidson; 2005.
[18]  R.J. N, Yu J.J.: Immunity to tuberculosis. Annual Review of Immunology 2004, 22:599-617.
[19]  Kouakou K, Schepetkin I, Jun S, Kirpotina LN: Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis. BMC complementary and alternative medicine 2013, 13(149): 1-19.
[20]  Reford P, Boonstra A, Read S, Pitt J: Enhanced protection to Mycobacterium tuberculosis infection in IL-10-deficient mice is accompanied by early and enhanced Th1 responses in the lung. Journal of Immunology 2010, 40(8):2200-2210.
[21]  Green A, DiFazio R: IFN-γ from CD4 T-Cells Is Essential for Host Survival and Enhances CD8 T-cell Function during Mycobacterium tuberculosis Infection. Journal of immunology 2013, 190(1):270-277.
[22]  Cavalcanti VWN, Brelaz M.C.A., de Andrade L.N.J.K., J.C. F, Pereira V.R.A.: Review Article, Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis,. Pulmonary Medicine Volume 2012.
[23]  Mattos AM, Almeida C: Increased IgG1, IFN-g, TNF-a and IL-6 responses to Mycobacterium tuberculosis antigens in patients with Tuberculosis are lower after chemotherapy.. International immunology 2010, 22(9):775-782.
[24]  O'Garra A., Redford P.S., McNab F.W., Bloom C.I., R.J. W, Berry M.P.R.: The Immune Response in Tuberculosis. Annual Review of Immunology 2013, 31:475-527.