American Journal of Microbiological Research
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American Journal of Microbiological Research. 2015, 3(4), 125-128
DOI: 10.12691/ajmr-3-4-1
Open AccessArticle

Genotyping of Pulmonary Mycobacterium tuberculosis Isolates from Sudan Using Spoligotyping

Muataz M. Eldirdery1, , Intisar E. Alrayah1, 2, Mona OA. Awad ElkareIm3, Fatima A. Khalid4, Asrar M A/Salam Elegail5, Nuha Y. Ibrahim5, Eman O M. Nour5, Rahma H. Ali5, Elena Hailu6, Markos Abebe6, Abraham Aseffa6, Najem Aldin M. Osman7, Maowia M. Mukhtar8, Nihad M A. Elhaj1 and Atif A. Elagib1

1Tropical Medicine Research Institute, National Center for Research, Khartoum, Sudan

2College of Applied Medical Science, Shaqra University, Shaqra, KSA

3Blood Transfusion Service, National Blood Transfusion Center, Khartoum, Sudan

4Tuberculosis Research Center, University of Kassala, Kassala, Sudan

5National Tuberculosis Reference Laboratory, National Laboratory of Public Health, Khartoum, Sudan

66Molecular biology Armauer Hansen Research Institute, Addis Ababa, Ethiopia

7Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan

88Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan

Pub. Date: July 06, 2015

Cite this paper:
Muataz M. Eldirdery, Intisar E. Alrayah, Mona OA. Awad ElkareIm, Fatima A. Khalid, Asrar M A/Salam Elegail, Nuha Y. Ibrahim, Eman O M. Nour, Rahma H. Ali, Elena Hailu, Markos Abebe, Abraham Aseffa, Najem Aldin M. Osman, Maowia M. Mukhtar, Nihad M A. Elhaj and Atif A. Elagib. Genotyping of Pulmonary Mycobacterium tuberculosis Isolates from Sudan Using Spoligotyping. American Journal of Microbiological Research. 2015; 3(4):125-128. doi: 10.12691/ajmr-3-4-1

Abstract

Tuberculosis (TB) remains a major public health problem worldwide due to its high risk of person-to-person transmission, morbidity and mortality [1]. Sudan has a high burden of tuberculosis. Spoligotyping (spacer oligonucleotide typing) a rapid method for genotyping of Mycobacterium tuberculosis using the principle of reverse hybridization. The ecology of the prevalent mycobacteria strain can vary depending on country and region. The aim of this study was to determine the genotyping of Mycobacterium tuberculosis isolated from Sudan using spoligotyping SPOLDB4. A total of 75 Mycobacterium tuberculosis sputum samples were collected from pulmonary Tuberculosis patients attending references Laboratories and diagnostic centers in Khartoum and Eastern Sudan in (2011-2013). The mycobacteria were genotyped using Spoligotyping technique and data obtained were analyzed and compared to the SPOLDB4 database. Among the 75 isolate analyzed, 57(76%) were identified by SPOLDB4 and 18 (24%) could not be matched to any lineages. The most prevalent genotype cluster was MANU2 38 (50.7%) followed by CASI Delhi 8 (10.7%). In the study SIT54 was the most common pattern 37 (49.3%) followed by SIT25 6(8%).

Keywords:
spoligotyping mycobacterium tuberculosis Sudan

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