American Journal of Microbiological Research
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American Journal of Microbiological Research. 2015, 3(2), 65-75
DOI: 10.12691/ajmr-3-2-5
Open AccessArticle

Isolation and Screening of Indigenous Bambara Groundnut (Vigna Subterranea) Nodulating Bacteria for their Tolerance to Some Environmental Stresses

Ngo Nkot Laurette1, , Ngo Bisseck Maxémilienne1, Fankem Henri1, Adamou Souleymanou2, Kamguia Kamdem1, Ngakou Albert3, Nwaga Dieudonné4 and Etoa François-Xavier4

1Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon

2Laboratory of Soil Microbiology, Biotechnology Centre, University of Yaounde I, Yaounde, Cameroon

3Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon

4Department of Microbiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

Pub. Date: March 22, 2015

Cite this paper:
Ngo Nkot Laurette, Ngo Bisseck Maxémilienne, Fankem Henri, Adamou Souleymanou, Kamguia Kamdem, Ngakou Albert, Nwaga Dieudonné and Etoa François-Xavier. Isolation and Screening of Indigenous Bambara Groundnut (Vigna Subterranea) Nodulating Bacteria for their Tolerance to Some Environmental Stresses. American Journal of Microbiological Research. 2015; 3(2):65-75. doi: 10.12691/ajmr-3-2-5

Abstract

Environmental stresses are important limiting factors for crops production. The aim of this experiment is to isolate Legume Nodulating Bacteria (LNB) obtained from root nodules of bambara groundnut (Vigna subterranea L.) plants and evaluate their performance under some environmental constraints. Samples were collected in Cameroon from three location sites of the Humid-forest zone: Logbessou in the Littoral region; Mfoua in the South and Boga in the Centre region. Nodulation of bambara groundnut was examined in plastic bags and root nodules were collected from seedling. After their isolation, the bacteria were confirmed as LNB by re-nodulating Macroptilium atropurpureum. The morphological, cultural and phenotypic characteristics (utilization of carbon, tolerance to salt, pH, aluminium) of isolates were determined. The results obtained were analyzed statistically by ANOVA using the software SPSS analysis version 11.5. Duncan test was used to measure the difference among the means at a level of p<0.05. A collection of 18 isolates was obtained on Yeast Extract Mannitol Agar medium. Authentication experiments, confirmed that the majority of the isolates (66.67%) were LNB due to their ability to infect the host plant. Bambara groundnut isolates are different morphologically. Dendrogram of the phenotypic characteristics showed that, below the boundary level of 50% average similarity, isolates fell into at least three distinct groups. All isolates showed fast-growing capacity. Most isolates (66.67%) were able to grow in a medium with pH as low as and Al concentration of 50 µM (58.33 %). Some isolates (50%) showed weak growth capacity at 4% NaCl. The bambara groundnut isolates tested were able to use a broad range of carbohydrates as sole source of carbon. The isolates from the present study may be useful to increase the symbiotic nitrogen fixation in legume.

Keywords:
bambara groundnut isolation legume nodulating bacteria phenotypic characterization

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