American Journal of Microbiological Research
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American Journal of Microbiological Research. 2015, 3(2), 50-54
DOI: 10.12691/ajmr-3-2-1
Open AccessArticle

Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan

Mona Mamoun1, , Elsanousi S. M.2, Khalid A. Enan3, Abdelmounem E. Abdo4 and Mohamed A. Hassan5, 6

1Department of Microbiology, Kassala University, Sudan

2Department of Microbiology, Khartoum University, Sudan

3Virology Department Central lab, Sudan

4Consultant Physician and Gastroenterology, Sudan

5Department of Bioinformatics, Africa City of Technology, Sudan

6Division of Molecular Genetics, Institute of Human Genetics, University of Tübingen, Germany

Pub. Date: March 03, 2015

Cite this paper:
Mona Mamoun, Elsanousi S. M., Khalid A. Enan, Abdelmounem E. Abdo and Mohamed A. Hassan. Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan. American Journal of Microbiological Research. 2015; 3(2):50-54. doi: 10.12691/ajmr-3-2-1

Abstract

H. pylori are a ubiquitous microorganism infecting up to half of the world’s population. A total of 81 gastric biopsies taken from patients complaining of gastric disorders in Khartoum state, Sudan screened for H.pylori. Eighteen samples (22.2%) yielded positive culture results. The majority of them were males. Also results indicated higher prevalence of H. pylori in patients with gastritis. Further identification performed using PCR targeted a region of 16S ribosomal RNA gene of H. pylori and gene amplified on 12 samples. Six of isolated sequences subjected to BLAST analysis that showed high similarity to GenBank strains of H. pylori.Multiple sequence alignments were performed between isolated 16S rRNA gene sequences and most related H.pylori strains deposited on GenBank. One isolate differed on one base-pair substitution (G-A) from other isolates and selected reference H.pylori strains. Phylogenetic analysis based on 16S rRNA gene sequences reflects that H.pylori could be originated from Africa.

Keywords:
Helicobacter pylori BLAST Phylogenetic 16s rRNA Sudan

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