American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
Open Access
Journal Browser
Go
American Journal of Microbiological Research. 2015, 3(1), 41-44
DOI: 10.12691/ajmr-3-1-6
Open AccessArticle

Evaluation of the Degree of Contamination of Salad Vegetables Sold in Umuahia Main Market

Nwankwo. I.U1, Eze. V.C1, Onwuakor. C.E1, and Friday. J.U1

1Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.

Pub. Date: February 01, 2015

Cite this paper:
Nwankwo. I.U, Eze. V.C, Onwuakor. C.E and Friday. J.U. Evaluation of the Degree of Contamination of Salad Vegetables Sold in Umuahia Main Market. American Journal of Microbiological Research. 2015; 3(1):41-44. doi: 10.12691/ajmr-3-1-6

Abstract

The evaluation of the degree of contamination of salad vegetables sold in Umuahia main market was carried out. Six Samples of salad vegetables (Cabbage, Carrot, Cucumber, Onions, Green peas and Green beans) sold around the market were analyzed. Total viable counts ranged from 2.35 x 105 to 9.50 x 105 cfu/ml with highest incidence seen in cabbage and onions sold by vendor A. 86.66% of cabbage samples were contaminated, with the least incidence seen in green bean (33.33%). Six bacterial and five fungal species were isolated. The isolates include: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus sp, Enterobacter sp and Proteus sp. and the fungal isolates were Aspergillus sp. Yeast, Cladosporium sp. Penicillium sp. and Rhizopus sp. These results indicates that street vended salad vegetables where contaminated to an extent, and therefore, needs vigorous washing with safe running water before consuming to avoid food borne diseases associated with consumption of contaminated food.

Keywords:
Evaluation Degree Of Contamination Salad Vegetables Umuahia Market

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Harli, V. and Franca, B. Fruits and Vegetables International Agency for Research on Cancers (IARC) Report 2, 2003.
 
[2]  Beuchat, L.R and Larry, R. Pathogenic Microorganism Associated with Fresh Produce. Journal of Food Protection 13:204-216. 2000.
 
[3]  Prigulkar, K.A., Kamat, O and Bougiruwar, D. Microbiology Quality of Fresh Leafy Vegetables, Salad Components and Ready to Eat Salads. Int. J. Food Science Nutrition 52: 15-23. 2000.
 
[4]  Beuchat, L.R Ecological Factor Influencing Survival and Growth of Human Pathogens on Raw Fruits and Vegetables Microb. Infection 4 (4): 413-423. 2002.
 
[5]  Liu, D. Identification, Sub typing and Virulence Determination of Listeria Monocytogenes, an Important Food Borne Pathogens. J. Med. Microbiol 55 (6): 645-659. 2006.
 
[6]  Kalia, A. and Gupta, R.P. Fruit Microbiology. In: Hui Y.H.J., Cano, M.P., Gusek, W., Sidhu, J.W., Sinha, N.K. Handbook of Fruit and Fruit Processing. 1st Edt. Blackwell Publishing, 2006, 3-28.
 
[7]  Carmo, L.S., Cumnings, C., Lenardi, V.R., Dias, R.S., Souza, J.M., Sena, M.J., Santos, D.A., Shupp, J.W., Pereira, R.K., Jett, M. A Case Study of a Massive Staphylococcal Food Poisoning Incident Food Borne Pathog. Dis 1:241-246. 2004.
 
[8]  Ray, B. and Bhunia, A.K. Fundamental Food Microbiology 4th Edt. CRC Press. U.S.A. 2007, 4-13.
 
[9]  Ofor, M.O., Okorie, V.C., Ibeawuchi, U., Ihejirika, G.O., Obilo, O.P., and Dialoka, S.A. Microbial Contaminant in Fresh Tomato Wash Water and Food Safety Consideration in South-Eastern Nigeria. Life Science J. 1:80-82. 2009.
 
[10]  Vishwanathan, P. and Kaur, R. Prevention and Growth of Pathogens on Salad Vegetables, Fruits and Sprouts. Intl. J. Hyg. Environ. Health 203: 205-213. 2000.
 
[11]  Beuchat, L.R Pathogenic Microorganisms Associated with Fresh Produce Journal of Food Protection 59: 204-216. 1996.
 
[12]  Vanderzant, C. and Splittoeser, D.F. Compendium of Methods for the Microbiological Examination of Foods Washington D.C. American Health Association, 1992, 44-61.
 
[13]  Beuchat, L.R and Brackelt, R.E. Survival and Growth of Listeria monocytogens on Lettuce as Influenced by Shredding, Chlorine Treatment, Modified, Packaging and Temperature Journal of Food Protection 55 (3): 755-758. 1998.
 
[14]  Leus, J., Venter, P. Westhuizen, J., and Vander, H., Enumeration of Potential Microbiology Hazards in Milk from a Marginal Urban Settlement in Central South Africa. Food Microbiology 321-326. 2002.
 
[15]  Bean, N.H. and Griffin, P.M. Food Borne Disease Outbreaks in the United States 1973-1987: Pathogens, Vehicles and Trends Journal of Food Protection 53: 807-814. 1992.