Proximate Composition, Biochemical and Microbiological Changes Associated with Fermenting African Oil Bean (Pentaclethra macrophylla Benth) Seeds

Eze V.C^1, , Onwuakor C.E¹ and Ukeka E¹

¹Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria

Cite this paper:
Eze V.C, Onwuakor C.E and Ukeka E. Proximate Composition, Biochemical and Microbiological Changes Associated with Fermenting African Oil Bean (Pentaclethra macrophylla Benth) Seeds . American Journal of Microbiological Research. 2014; 2(5):138-142. doi: 10.12691/ajmr-2-5-3

Abstract

The proximate composition, biochemical changes and microbiology of fermenting Pentaclethra macrophylla (Ugba) seeds were evaluated. Studies were carried out to screen for microorganisms associated with the natural fermentation of the oil bean seeds. Bacterial isolates obtained include species of Bacillus, Streptococcus, Salmonella, Micrococcus, Lactobacillus and Proteus. Fungal isolates include Yeast, species of Penicillium, Aspergillus, Fusarium and Rhizopus. Total aerobic counts (TAC) ranged from 1.5 x 10⁶ to 2.5 x 10⁶ cfu/g, while total coliform counts (TCC) ranged from 1.7 x 10³ to 7.2 x 10³ cfu/g. More so, total lactic acid bacterial counts ranged from 2.6 x 10⁵ to 4.6 x 10⁵ cfu/g. Among the various bacterial isolates obtained from the fermenting Ugba, Bacillus and lactic acid bacteria were dominant from the beginning to the end of the fermentation the oil bean seeds. The proximate composition of the fermenting seeds showed the presence of protein, fats, fibre, carbohydrates, and ash. Temperature variations in oil bean seed fermentation showed higher temperatures in the purchased Ugba compared to the laboratory Ugba after 72 hours fermentation. There were significant reduction in pH and titratable acidity as the fermentation time progressed, showing that temperature, pH and titratable acidity of fermenting African oil bean seeds were affected by the metabolic activities of resident microorganisms.

Keywords:
Pentaclethra macrophylla Ugba proximate biochemical temperature titratable acidity

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