American Journal of Microbiological Research
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American Journal of Microbiological Research. 2014, 2(5), 125-130
DOI: 10.12691/ajmr-2-5-1
Open AccessArticle

Effect of Varied Culture Conditions on Crude Supernatant (Bacteriocin) Production from Four Lactobacillus Species Isolated from Locally Fermented Maize (Ogi)

C.E Onwuakor1, , V.O Nwaugo2, C.J Nnadi2 and J.M Emetole3

1Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria

2Department of Microbiology, Faculty of Biological & Physical Sciences, Abia State University Uturu, Abia State, Nigeria

3Department of Product Development Programme, National Root Crop Research Institute Umudike, Abia State, Nigeria

Pub. Date: September 04, 2014

Cite this paper:
C.E Onwuakor, V.O Nwaugo, C.J Nnadi and J.M Emetole. Effect of Varied Culture Conditions on Crude Supernatant (Bacteriocin) Production from Four Lactobacillus Species Isolated from Locally Fermented Maize (Ogi). American Journal of Microbiological Research. 2014; 2(5):125-130. doi: 10.12691/ajmr-2-5-1

Abstract

This Lactic acid bacteria (LAB) predominates the micro flora of fermented products. They produce metabolites that inhibit the growth of food borne pathogens and spoilage microorganisms. The isolation and identification of LAB from fermented maize (Ogi) and the effect of varied culture conditions on crude supernatant production and activity was evaluated. Four (4) isolates of bacteriocin producing lactobacillus species (L. lactis, L. fermentum, L. casei and L. plantarum) with antibacterial activity against Salmonella typhimurium (ATCC 14028) and Shigella dysenteriae (ATCC 23351) were subjected to varying growth medium conditions. The crude supernatant production was tested at different physical and cultural conditions such as temperature (25, 30, 35 and 40°C), pH (5, 6, 7 and 8), sodium chloride (NaCl) concentration (2, 4, 6 and 8%) and incubation duration (12, 24, 48 and 72 hours). The optimum bacteriocin production judged by their different zones of inhibition of crude supernatant was recorded at temperature, 30°C and then 35°C. There were significant differences between all the incubation temperatures at P<0.05. Duration of incubation showed highest crude supernatant activity after 72 hours. Furthermore, optimal conditions for crude supernatant production were observed to be highest at pH 6.0 followed by 5.0 and then 2% NaCl concentration. There were significant differences between the zones of inhibition of crude supernatants produced against the indicator organisms at various media pH and salt concentrations at P<0.05. These crude supernatants may have a potential use in reducing contaminations during industrial processes, as food preservatives and may help in improving the gastro-intestinal tract by fighting off pathogenic bacteria.

Keywords:
varied culture crude supernatant bacteriocin production lactobacillus fermented maize Ogi

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References:

[1]  Abdulmumeen, H.A., Risikat, A.N and Sururah, A.R. Food: Its preservatives, additives and applications. International Journal of Chemical and Biological Sciences, 2012, 1: 36-47.
 
[2]  Francis, F.J. Pioneer in Food Sciences and Quality, In: A Century of Food Science. Institute of Food Technologists. 2000.
 
[3]  Savadogo, A., Quattara, C.A.T., Bassole, I.H.N and Traore, S.A. Bacteriocin and lactic acid bacteria, a mini review. African Journal of Biotechnology. 2006, 5 (9): 678-683.
 
[4]  De Vuyst, L and Leroy, F. Bacteriocins from lactic acid bacteria: production, Purification and food Applications. Journal of Molecular Microbiology and Biotechnology. 2007, 13: 194-199.
 
[5]  Ross, R.P., Morgan S and Hill, C. Preservation and fermentation: Past, present and future. International Journal of Food Microbiology. 2002, 79: 3-6.
 
[6]  El-Ziney, M.G., Debevere, J and Jakobsen, M. Reuterin, In: Naidu, A.S (Ed.), Natural Food Antimicrobial Systems. CRC Press, London. 2000, 567-587.
 
[7]  Galvez, A., Abriouel, H., Lopez, R.L and Omar, N.B. Bacteriocin based strategies for food bio-preservation. International Journal of Food Microbiology. 2007, 120: 51-70.
 
[8]  Klaenhammer, T.R. Genetics of bacteriocins produced by lactic acid bacteria, FEMS Microbiology Reviews. 1993, 12: 39-85.
 
[9]  Tagg, A. S. Dajani, A.S and Wannamaker, L.W. Bacteriocins of Gram positive bacteria. Bacteriological Reviews. 1976, 40: 722-756.
 
[10]  Adams, M.R and Moss, M.O. Food Microbiology. 3rd ed. Athenaeum Gateshead, Tyne and Wear, London. 1995, 227-239.
 
[11]  Amakoromo, E.R. Indigenous Fermented Foods of Nigeria: Processing, composition and Improvement. University of Port Harcourt Press, PH, Nigeria. 2011, 57-65.
 
[12]  Ozoh, P.T.E and Kuyanbana, Z.U. Microbial quality of pap prepared from cereals sold in Bauchi markets, Nigeria. Int. J. Environ. Health, Res. 1995, 5: 133-141.
 
[13]  Marero, L.M, Pagumo, E.M., Aguinaldo, A.R and Homma, S. Nutritional characteristics of weaning foods prepared from germinated cereals and legumes. J. Food Sci. 1989, 53(8): 1399-1402.
 
[14]  Mbakwem- Aniebo, C and Udemgba, G. Microbiological quality of untreated and salt-treated Ogi (Akamu) kept at room temperature. Nature and Science. 2012, 10(8): 26-29.
 
[15]  Akinrele, I.A. Fermentation studies on maize during the preparation of a traditional African Starch-cake food. J. Sci. Food Agric. 1970, 21: 619-625.
 
[16]  Odunfa, S.A. African fermented foods. In: Microbiology of Fermented Foods. (Wood, B.J. (Ed.)). Elsevier Applied Science Publishers, New York. 1985, 25-42.
 
[17]  Axelsson, L. Lactic acid bacteria: classification and physiology. In Advance in Lactic Acid Bacteria: Microbiological and Functional Aspects, Salminen, S., Von Wright, A and Ouweland A Editors. Marcel Dekker, New York, U.S.A. 2004, 1-66.
 
[18]  Charteris, W.P., Kelly, P.M., Morelli, L and Collins, J.K. Quality control Lactobacillus isolates for use with the API50 CH and API ZYM systems at 37°C. J. Basic Microbiol. 2001, 41, 241-251.
 
[19]  Klein, G. International Committee of Systematic Bacteriology, subcommittee on the taxonomy of bifidobacterium, lactobacillus and related organisms. Minutes of the meeting. Int. J. Syst. Evol. Microbiol. 2001, 51, 259-1.
 
[20]  Harrigan, W.F and McCance, M.E. Laboratory Methods in Food and Dairy Microbiology, second ed. 1976, Academic Press, London.
 
[21]  Schillinger, U and Lucke, F.K. Antibacterial activity of Lactobacillus sakei isolated from meat. Applied and Environmental Microbiology, 1989, 55: 1901-1906.
 
[22]  Ogunshe, A.A.O., Omotoso, M.A and Adeyeye, A. In vitro antimicrobial characteristics of bacteriocin – producing Lactobacillus strains from Nigerian indigenous fermented foods. African Journal of Biotechnology. 2007, 2(8): 219-227.
 
[23]  Sarika, A.R., Lipton, A.P and Aishwarya, M.S. Bacteriocin production by new isolate of Lactobacillus rhamnosus GP1 under different culture conditions. Advance Journal of food Science and Technology. 2010, 25(5): 291-297.
 
[24]  Aly, S., Cheik, A.T., Ouattara, I., Bassole, H.N and Alfred, S.T. Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk. Pakistan Journal of Nutrition. 2004, 3(3): 174-179.
 
[25]  Sourav, B and Arijit, D. Study of Physical and cultural Parameters on the bacteriocins produced by lactic acid bacteria Isolated from Traditional Indian fermented Foods. American Journal of Food Technology. 2010, 5(2): 111-120.
 
[26]  Karaoglu, A.S., Faruk, A., Kilic, S.S and Kilic, A.O. Antimicrobial activity and characteristics of bacteriocins produced by vaginal lactobacilli. Turkish Journal of Medical Sciences. 2003, 33: 7-13.
 
[27]  Divakara, R., Manjunatha, B.K and kusum, P. Lactic acid bacteria as probiotics: Role in human health. Research Reviews in Biomedicine and Biotechnology. 2010, 1(1): 1-5.
 
[28]  Zhao, C., Beilei, G., Vilena, J., Sudler, R., Yeh, E., Zhao, S., White, D.G., Wagner, D and Meng, A. Prevalence of Campylobacter spp., Escherichia coli and Salmonella serovars in retail chicken, turkey, pork and beef from the greater Washington DC area. Applied and Environmental Microbiology. 2000, 66: 5431-5436.
 
[29]  Onwuakor, C.E and Ukaegbu-Obi, K.M. Synergistic Bio-preservative Effects of Vernonia amygdalina Leaves and Sacoglottis gabonensis Stem Bark on Palm Wine from Elaeis guineensis and Raphia hookeri from Uturu, Nigeria.” American Journal of Microbiological Research. 2014, 2(3): 105-109
 
[30]  Ogunshe, A.A.O., Ayodele, A.E and Okonko, O. Microbial studies on Aisa: A potential indigenous fermented food condiment from Albiziz saman (Jacq.). Pakistan Journal of Nutrition. 2005, 5(1): 51-58.
 
[31]  Okereke, H.C, Achi, O.K, Ekwenye, U.N and Orji, F.A. Antimicrobial properties of probiotic bacteria from various sources. African Journal of Biotechnology. 2012, 11(39): 9416-9421.
 
[32]  Schnurer, J and Magnusson, J. Antifungal lactic acid bacteria as bio-preservatives. Trends in Food Science and Technology. 2005, 16: 70-78.
 
[33]  Settanni, L and Corsetti, A. Application of bacteriocins in vegetable food bio-preservation. International Journal of Food Microbiology. 2008, 121: 123-138.