American Journal of Microbiological Research
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American Journal of Microbiological Research. 2014, 2(5), 125-130
DOI: 10.12691/ajmr-2-5-1
Open AccessArticle

Effect of Varied Culture Conditions on Crude Supernatant (Bacteriocin) Production from Four Lactobacillus Species Isolated from Locally Fermented Maize (Ogi)

C.E Onwuakor1, , V.O Nwaugo2, C.J Nnadi2 and J.M Emetole3

1Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria

2Department of Microbiology, Faculty of Biological & Physical Sciences, Abia State University Uturu, Abia State, Nigeria

3Department of Product Development Programme, National Root Crop Research Institute Umudike, Abia State, Nigeria

Pub. Date: September 04, 2014

Cite this paper:
C.E Onwuakor, V.O Nwaugo, C.J Nnadi and J.M Emetole. Effect of Varied Culture Conditions on Crude Supernatant (Bacteriocin) Production from Four Lactobacillus Species Isolated from Locally Fermented Maize (Ogi). American Journal of Microbiological Research. 2014; 2(5):125-130. doi: 10.12691/ajmr-2-5-1


This Lactic acid bacteria (LAB) predominates the micro flora of fermented products. They produce metabolites that inhibit the growth of food borne pathogens and spoilage microorganisms. The isolation and identification of LAB from fermented maize (Ogi) and the effect of varied culture conditions on crude supernatant production and activity was evaluated. Four (4) isolates of bacteriocin producing lactobacillus species (L. lactis, L. fermentum, L. casei and L. plantarum) with antibacterial activity against Salmonella typhimurium (ATCC 14028) and Shigella dysenteriae (ATCC 23351) were subjected to varying growth medium conditions. The crude supernatant production was tested at different physical and cultural conditions such as temperature (25, 30, 35 and 40°C), pH (5, 6, 7 and 8), sodium chloride (NaCl) concentration (2, 4, 6 and 8%) and incubation duration (12, 24, 48 and 72 hours). The optimum bacteriocin production judged by their different zones of inhibition of crude supernatant was recorded at temperature, 30°C and then 35°C. There were significant differences between all the incubation temperatures at P<0.05. Duration of incubation showed highest crude supernatant activity after 72 hours. Furthermore, optimal conditions for crude supernatant production were observed to be highest at pH 6.0 followed by 5.0 and then 2% NaCl concentration. There were significant differences between the zones of inhibition of crude supernatants produced against the indicator organisms at various media pH and salt concentrations at P<0.05. These crude supernatants may have a potential use in reducing contaminations during industrial processes, as food preservatives and may help in improving the gastro-intestinal tract by fighting off pathogenic bacteria.

varied culture crude supernatant bacteriocin production lactobacillus fermented maize Ogi

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