American Journal of Microbiological Research
ISSN (Print): 2328-4129 ISSN (Online): 2328-4137 Website: http://www.sciepub.com/journal/ajmr Editor-in-chief: Apply for this position
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American Journal of Microbiological Research. 2014, 2(4), 113-117
DOI: 10.12691/ajmr-2-4-2
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Synergistic Bio-preservative Effects of Vernonia Amygdalina Leaves and Sacoglottis Gabonensis Stem Bark on Palm Wine from Elaeis Guineensis and Raphia Hookeri from Uturu, Nigeria

Onwuakor Chijioke .E1, and Ukaegbu-Obi K.M1

1Department of Microbiology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria

Pub. Date: July 08, 2014

Cite this paper:
Onwuakor Chijioke .E and Ukaegbu-Obi K.M. Synergistic Bio-preservative Effects of Vernonia Amygdalina Leaves and Sacoglottis Gabonensis Stem Bark on Palm Wine from Elaeis Guineensis and Raphia Hookeri from Uturu, Nigeria. American Journal of Microbiological Research. 2014; 2(4):113-117. doi: 10.12691/ajmr-2-4-2

Abstract

The synergistic bio-preservative effects of leaves and stem bark of Vernonia amygdalina and Sacoglottis gabonensis respectively on two (2) palm wine types namely, Elaeis guineensis and Raphia hookeri from Uturu-Nigeria was evaluated. The microbiological and biochemical changes of the palm wine brands were determined. R. hookeri brands were found to support more heterotrophic and coliform populations than the E. guineensis, while the later contained more yeast species. Identification of isolated species revealed the presence of Bacillus sp., Micrococcus sp., Lactobacillus sp., Brevibacterium sp. and Saccharomyces sp. from E. guineensis and R. hookeri. Moreso, heterotrophic counts and pH were observed to decrease as the fermentation time progressed. The combination of both V. amygdalina and S. gabonensis as preservatives lowered the bacterial and fungal load compared to the control and individual plant preservatives and reduced the rate of CO2 emission as well as keeping the pH fairly constant.

Keywords:
synergistic bio-preservative Vernonia amygdalina Sacoglottis gabonensis palm wine Uturu

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