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American Journal of Microbiological Research. 2014, 2(1), 47-51
DOI: 10.12691/ajmr-2-1-7
Open AccessArticle

Influence of Aeration Speed on Bacterial Colony Forming Unit (CFU) Formation Capacity

Md. Sakil Munna1, Sadika Tamanna1, Most. Rumana Afrin1, Gulshan Ara Sharif1, Chaity Mazumder1, Konica Sarker Kana1, Nusrat Jahan Urmi1, Md. Aftab Uddin1, Tasmina Rahman1 and Rashed Noor1,

1Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh

Pub. Date: February 09, 2014

Cite this paper:
Md. Sakil Munna, Sadika Tamanna, Most. Rumana Afrin, Gulshan Ara Sharif, Chaity Mazumder, Konica Sarker Kana, Nusrat Jahan Urmi, Md. Aftab Uddin, Tasmina Rahman and Rashed Noor. Influence of Aeration Speed on Bacterial Colony Forming Unit (CFU) Formation Capacity. American Journal of Microbiological Research. 2014; 2(1):47-51. doi: 10.12691/ajmr-2-1-7


Bacterial homeostasis depends on an array of physical and chemical stimulants. Current investigation assessed the impact of one of such factors, the speed of aeration, on cell viability and culturability of Escherichia coli, Pseudomonas spp. and Bacillus spp. Each of the bacterial strain was incubated at 37°C with a shaking speed of 0, 100 or 200 rotation per minute (rpm) separately up to 72 hours, with a simultaneous monitoring of morphological changes and cell culturability. All bacterial species were found to optimally grow at 100 rpm whereas at 0 rpm growths of E. coli and Pseudomonas spp. were bit slower compared to that of Bacillus spp. The capacity to form colony forming units (CFUs) of E. coli and Pseudomonas spp. on Luria Burtani (LB) agar plates were observed to be inhibited after 36 hours of growth at 200 rpm; i.e., approximately 3-log reduced CFUs than those formed by Bacillus spp. Besides, morphologically impaired cells were observed for the former two bacteria cultivated at 200 rpm. Taken together, it is assumed that the high speed shaking might evolve the oxidative stress endogenously which possibly rendered the cells lose their culturability.

Eshecrichia coli Pseudomonas spp. Bacillus spp. aeration speed colony forming units (CFUs) oxidative stress

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