American Journal of Microbiological Research
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American Journal of Microbiological Research. 2020, 8(1), 24-33
DOI: 10.12691/ajmr-8-1-4
Open AccessArticle

Application of Hazard Analysis Critical Control Point (HACCP) Concept Using Sodium Metabisulphite and Hypochlorite to Enhance Microbial Safety of Shrimps

Bernard Johnson Okpako Efiuvwevwere1, , Chimezie Jason Ogugbue1, Godwin Emoghene2 and Augustine Kpeedee Ngbara-ue2

1Department of Microbiology, Faculty of Science,University of Port Harcourt, Port Harcourt, Nigeria

2Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria

Pub. Date: February 23, 2020

Cite this paper:
Bernard Johnson Okpako Efiuvwevwere, Chimezie Jason Ogugbue, Godwin Emoghene and Augustine Kpeedee Ngbara-ue. Application of Hazard Analysis Critical Control Point (HACCP) Concept Using Sodium Metabisulphite and Hypochlorite to Enhance Microbial Safety of Shrimps. American Journal of Microbiological Research. 2020; 8(1):24-33. doi: 10.12691/ajmr-8-1-4

Abstract

Shrimps are highly valued worldwide. However, they deteriorate rapidly after harvest except preserved or subjected to hazard analysis critical control point (HACCP). Therefore, this investigation was undertaken to determine the microbiological and physico-chemical [pH and trimethylamine (TMA)] characteristics of shrimps subjected to several critical control points (CCPs) including 100 ppm sodium metabisulphite (Na2S2O5) alone or followed by 10 ppm calcium hypochlorite Ca[OCL]2 before ambient (27-35°C) or refrigeration (4-6°C) storage. Also, shrimp types (whole, head or tail subjected to CCPs (iced or un-iced storage) wereanalysed for total viable counts (TVCs), coliforms, Staphylococcus spp.,Salmonella spp. and Vibrio spp. counts; pH and TMA contents. Significant (p<0.05) maximum TVCs of 1.8 x 108cfu/g occurred in tail samples subjected to Na2S2O5 followed by Ca[OCL]2 before 27-35°C storage. Significant different bacterial populations occurred with the un-iced whole shrimps showing maximum population (2.9 x 105cfu/g) of coliforms. Additionally, Staphylococcus spp. had the maximum count (8.2 x 103cfu/g) in un-iced whole samples while the lowest (7.8 x 101cfu/g) occurred in tail samples treated with Na2S2O5 followed by Ca[OCL]2 before 4-6°C storage. Variations in bacterial profiles were influenced by the CCPs resulting in diverse bacteria with iced head samples showing Bacillus spp.,E.coli, Pseudomonas spp., Salmonella spp. and Staphylococcus aureus while the others differed. Most bacterial pathogens occurred in tail samples subjected to CCPs before 27-35°C storage. Highest pH (7.85) and TMA (37.48mgN/100g) occurred in tail samples treated with Na2S2O5 followed by Ca[OCL]2 before ambient storage. Significant positive correlation occurred between TVCs and coliforms (r = 0.9011) and others. However, pH and TMA showed negative or poor correlation against the different bacterial groups.Percentage frequency of bacterial occurrence differed. This study has demonstrated the importance of HACCP and the need to adopt its concept and application to enhance microbial safety of shrimps.

Keywords:
shrimps HACCP critical control measures microbial safety

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References:

[1]  Omar, M. I. V. Utilization of sodium metabisulphite for preservation of frozen thaivel shrimp (Pandaleus borealis). The United Nations University, Fisheries Training Programme, Iceland, Final Project 1998.
 
[2]  Jimoh, A.A. and Lemomu, L.P. Shell fish resources in Nigeria. Proceedings of Fisheries, Society of Nigeria (FISON), ASCON, Badagry, 25th -29th Oct. 2010; 683-693.
 
[3]  Ramesh B.K., Govindaw, R.V Krishna, N.M Geetha, S. and Kakara, R.R Assessment of bacteriological quality in selected commercially important shrimps of Visakhapatnawm, East coast of India.International J.Microbiological Biotechnology 2017; 2(2): 102-105
 
[4]  Jay, J. M. Modern Food Microbiology 6th edition Aspen; Maryland USA. 2000.
 
[5]  Condurso, C. Tripodi, G; Cincotta, F; Lanza, C.M Mazzagha, A. and Verzera, A. Quality Assessment of Mediterranean shrimps during storage. Italian J. Food Science 2016; 28: 497-509.
 
[6]  Samia, S; Galib, H.T., Tanvir, A. S; Basudob, C.S; Walliullah, M.D; Tasniaw, A; Sakil, M.D; Afsana, F.N; Sadia, K.P Kamal, K.D; Mrityunjoy, A Nusrat, J.UT asminia, R. and Rashed, N. Microbiological quality analysis of shrimps collected from local market around Dhaka city. International Food Research J. 2014; 21(1): 33-38.
 
[7]  Miget, R. Shellfish handling practices-shrimps and molluscs. Southern Regional Aquaculture center, (SRAC), Texas. SRAC Publication No. 4902. May 2010.
 
[8]  Jonnalagadda, P.R. Sudershan, R.V., Raji, N.S. and Rao, D.R. Identification of critical control points in the two selected HACCP-certified prawn processing units. J. Food Quality 2009; 32:177-189.
 
[9]  Ronholm, J., Lau, F. and Banerjee, S.K Emerging seafood preservation techniques to extend freshness and minimize Vibrio contamination: Frontiers in Microbiology 2016; 7:1-7.
 
[10]  Martinez, Alvarez, O Gomez-Gullen, M.C and Montero, P. Role of sulfites and 4-hexylresorcinol in microbial growth and melanosis prevention of deepwater pink shrimp (Parapenaculongirostris) using a controlled atmosphere. J. Food Protection, 2005; 68 (1): 98-104.
 
[11]  Pyle M.L. and Koburger J.A. Increased sensitization of shrimp microflora to hypochlorite following a sodium bisulphite dip. J. Food Protection 1984; 47 (5): 375-377.
 
[12]  Khodanazary, A. Freshness assessment of shrimp Metapenaeusaffnis by quality index method and estimation of its shelf life. International J. Food Properties 2019; 22(1): 309-319.
 
[13]  Ehiri, J.E Azubuike, M.C Ubbaonu, C.NAnyanwu, E. C Ibe, K. M. and Ogbonna, M.O. Critical control points of complementary food preparation and handling in Eastern Nigeria. Bulletin World Health Organization. 2001; 7(5): 423-435.
 
[14]  Nahemiah, D; Bankole, O.S; Tswako, MNma-Usman, K.I Hassan, H. and Fati, K.I. Hazard analysis critical control points (HACCP) in the production of soy-Kununzaki: A traditional cereal-based fermented beverage of Nigeria. American Food Science Technology. 2014; 2(6): 196-202.
 
[15]  Cheesbrough M. District Laboratory Practice in Tropical countries Part 2. Cambridge University Press, 2000; 400-434.
 
[16]  APHA (American Public Health Association). In F.P. Downes and K. Ito (Eds).Compendium of methods for the microbiological examination of foods.(4th edition).Washington, DC; American Public Health Association; USA. 2001.
 
[17]  Sneath, P. H.A; Mair, N.S. Sharpe, M.E and Halt, J.G. Bergey’s Manual of Systemic Bacteriology vol. 2. Williams and Wilkins; Baltimore, 1986.
 
[18]  Oruwari, B.O. and Efiuvwevwere, B.J.O. The effects of different storage temperatures on the microbial, physico-chemical and organoleptic quality changes in the shellfish “Ngolo” (Thais-califera) from Nigeria. British Microbiology Research J.2016; 16(2):1-11.
 
[19]  Malle, P and Poumeyrol, M.A. New chemical criterion for determination of trimethylamine and total volatile base nitrogen.J. Food Protection1989; 52:419-423.
 
[20]  FAO/WHO Guidance to governments on the application of HACCP in small and/or less-developed food businesses. FAO Food and Nutrition: Paper 86; 2014; FAO/WHO, Geneva.
 
[21]  Sudheer, K.P; Sankalpa, K.B. and Saranya, S. Effect of preservatives and temperature on microbial and physico-chemical attributes of minimally processed pineapple. International J. Current Microbiology. Applied Sciences 2019; 8(2):541-553.
 
[22]  Depaola, A Jones, J. L Woods, J. Burkhardt, W Cala, K.R Krantz, J.A, Bowers, J.C., Kasturi, K Byara, R.H. Jacobs, E. Williams-Hill, D. and Nabe, K. Bacterial and viral pathogens in live oysters: 2007 United States Market survey. Applied and Environmental Microbiology. 2010; 7b(9): 2754-2768.
 
[23]  International Commission on Microbiological Specification for Foods (ICMSF). Microorganisms in Foods. 2. Sampling for Microbiological Analysis. Principles and Specific Applications.2nd edition.Blackwell Scientific Publications 1986.
 
[24]  FAO Corporate Document on Repository Assessment and Management of Seafood Safety and Quality (EEC 93/493); 1993.
 
[25]  Gram, L. and Huss, H.H. Microbiological spoilage of fish and fish products. International J.Food Microbiology 1996; 33:121-137.
 
[26]  Efiuvwevwere, B.J.O. and Amadi, L.O. Effects of preservatives on the bacteriological, chemical and sensory qualities of mangrove oyster (Crassostreagasar). British J. Applied Science Technology 2015; 5(1): 76-84.
 
[27]  Cahill, S. M. and Jouve, J.L. R. Microbiological risk assessment in developing countries. J. Food Protection 2004; 67(9): 2016-2023.