American Journal of Microbiological Research
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American Journal of Microbiological Research. 2019, 7(3), 78-82
DOI: 10.12691/ajmr-7-3-2
Open AccessArticle

Mycoflora and Public Health Risks of Smoked Fish Sold in Port Harcourt Markets, Nigeria

Akani Nedie Patience1, and Nwankwo Chidiebele Emmanuel Ikechukwu2

1Department of Microbiology, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, 500272 Rivers State, Nigeria

2Natural Science Unit, School of General Studies, University of Nigeria, Nsukka, 410002, Enugu State, Nigeria

Pub. Date: July 11, 2019

Cite this paper:
Akani Nedie Patience and Nwankwo Chidiebele Emmanuel Ikechukwu. Mycoflora and Public Health Risks of Smoked Fish Sold in Port Harcourt Markets, Nigeria. American Journal of Microbiological Research. 2019; 7(3):78-82. doi: 10.12691/ajmr-7-3-2

Abstract

Fish is a preferred source of protein globally, especially in developing countries like Nigeria. It is a savoured protein source in the Niger Delta, including Port Harcourt. Smoking is used to preserve fish by reducing its moisture content with a view to improved shelf life. This study aimed at determining the Mycoflora and the Public Health risks of smoked fish sold in Port Harcourt Markets. A total of 54 fish samples were collected from three strategic markets; Mile one, Oil Mill and Creek Road markets. Fish collected consists of 6 different species; Gadus morhua, Pseudotolithus typhus, Lutijanus goreensis, Ethalmosa fimbriata, Pseudotolithus senegalensis and Dasyatis pastinaca. All samples were grouped accordingly. Mycological study of fish samples was done using standard methods on Sabouraud Dextrose Agar. There was a significant difference in the mycoflora counts of smoked fish from different markets (p<0.05). Fungal load ranged from 1.23±0.08 x103 sfu/g in Lutjanus goreensis to 8.89±0.10 x 103 sfu/g in Gadus morhua, at Creek road market. From Mile 1 market, Lutjanus goreensis still hosted the highest population of 13.25±0.7 x 103 sfu/g and Dasyatis pastinaca had the least; 0.66±0.01 x 103 sfu/g. At Oil mill market, Ethalmosa fimbriata hosted 13.23±0.47 x 103 sfu/g while Gadus morhua had 0.77±0.02 x 103sfu/g. The fungal load in all fish from all three markets were significantly high for food and calls for attention. Nine fungal genera; Saccharomyces spp, Rhizopus spp, Penicillium spp, Mucor spp, Fusarium spp, Cladosporium spp, Candida spp, Absidia spp and Aspergillus spp, were isolated. All six fish species studied recorded more than 50 % occurrence of fungal species in all the markets. The mycoflora of smoked fish sold in Port Harcourt markets suggest significant public health risks. The need for improved storage and handling of this important protein source is high towards reduced public health risk. Proper preparation method, such as boiling, is strongly advocated.

Keywords:
Mycoflora public health risks smoked fish port harcourt markets

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References:

[1]  Ekong, F., Jacob, A., & Ebong, S. Water resource management in the Niger Delta region of Nigeria: The role of physical planning. Int. Rev. Soc. Sci. Human, 3(1), 51. February 2012.
 
[2]  Anihouvi, D. G. H., Kpoclou, Y. E., Abdel Massih, M., Iko Afé, O. H., Assogba, M. F., Covo, M., & Mahillon, J. Microbiological characteristics of smoked and smoked–dried fish processed in Benin. Food Science & Nutrition, 7, 1821-1827. April 2019.
 
[3]  Adeyemi, O. T., Osilesi, O. O., Onajobi, F., Adebawo, O., & Afolayan, A. J. Stability study of smoked fish, horse mackerel (Trachurustrachurus) by different methods and storage at room temperature. African Journal of Biochemistry Research, 7(6), 98-106. June 2013.
 
[4]  Nwankwo, C. E., & Akani, N. P. Bacterial flora of Clarias gariepinus from some selected fish ponds in Port Harcourt. Journal of Environment & Biotechnology Research, 6(2), 208-212. April 2017.
 
[5]  Samuel, O., Ifeanyi, O., & Ugochukwu, O. Mycoflora of smoke-dried fish varieties sold in Eke Awka Market in Anambra State, Nigeria. American Journal of Life Science Researches, 3(3), 196-201. August 2015.
 
[6]  Sani, F. M., Nasir, I. A., & Torhile, G. Mycological evaluation of smoked-dried fish sold at Maiduguri Metropolis, Nigeria: preliminary findings and potential health implications. European Journal of Health Science, 2(1), 5-10. June 2016.
 
[7]  Adebayo-Tayo, B. C., Onilude, A. A., & Patrick, U. G. Mycoflora of smoke-dried fishes sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences, 4(3), 346-350. March 2011.
 
[8]  Wogu, M. D., & Iyayi, A. D. Mycoflora of some smoked fish varieties in Benin City Nigeria. Ethiopian Journal of Environmental Studies and Management, 4(1), 36-38. January 2011.
 
[9]  Tongo, I., Ogbeide, O., & Ezemonye, L. Human health risk assessment of polycyclic aromatic hydrocarbons (PAHs) in smoked fish species from markets in Southern Nigeria. Toxicology reports, 4, 55-61. December 2017.
 
[10]  Atanda, O., Makun, H. A., Ogara, I. M., Edema, M., Idahor, K. O., Eshiett, M. E., & Oluwabamiwo, B. F. Fungal and mycotoxin contamination of Nigerian foods and feeds. In Mycotoxin and food safety in developing countries. Intech Open. January 2013.
 
[11]  Meka, I. A., Omotowo, B. I., Ijoma, U. N., Okoli, E. V., Nwagha, T. U., Ndu, A. C., & Ugwu, E. O. Zero Prevalence of Hepatitis B and C Coinfection among a Large Cohort of Health care Workers in Enugu, Nigeria: a Cross-sectional Descriptive Study. International Journal of Medicine and Health Development, 23, 171-175. May 2018.
 
[12]  World Health Organisation (WHO) Aflatoxins. Food Safety Digest, Department of Food safety and Zoonoses. REF. No.: WHO/NHM/FOS/RAM/18.1. 2018. Available at: https://www.who.int/foodsafety/FSDigest_Aflatoxins_EN.pdf. Accessed 01-06-2019.
 
[13]  Cheesebrough, M. Biochemical tests to identify bacteria. District Laboratories Practice in Tropical Countries, 2nd ed. Cambridge university press, Cape Town. pp. 62-70. 2006.
 
[14]  Ineyougha, E. R., Orutugu, L. A., &Izah, S. C. Assessment of Microbial Quality of Smoked Trachurustrachurus sold in some Markets of Three South-South States of Nigeria. International Journal of Food Research, 2, 16-23. May 2015.
 
[15]  Akande, G. R., & Tobor, J. G. Improved utilization and increased availability of fishery products as an effective control of aggravated animal protein deficiency-induced malnutrition in Nigeria. 10th Annual Conference of the Fisheries Society of Nigeria (FISON) 1: 18-31. November 1993.
 
[16]  Likongwe, M. C., Kasapila, W., Katundu, M., & Mpeketula, P. Microbiological quality of traditional and improved kiln smoked catfish (Clarias gariepinus; Pisces; Clariidae) in Lake Chilwa Basin. Food Sscience & Nutrition, 7(1), 281-286. January 2019.
 
[17]  Stołyhwo, A., & Sikorski, Z. E. Polycyclic aromatic hydrocarbons in smoked fish–a critical review. Food Chemistry, 91(2), 303-311. June 2005.
 
[18]  Sousa, A. K. A. D., Fogaça, F. H. D. S., Araujo, T. D. S., Castro, K. N. D. C., Pereira, a. M. L., Canuto, K. M., & Magalhães, J. A. D.Effect of Alpinia zerumbet essential oil on the shelf life of tambaqui fillets during short-term refrigerator storage. Acta Amazonica, 49(2), 152-161. May 2019.
 
[19]  Nwaerema, P., Vincent, O. N., Amadou, C., & Atuma, I. M. (2019). Spatial Assessment of Land Surface Temperature and Emissivity in the Tropical Littoral City of Port Harcourt, Nigeria. International Journal of Environment and Climate Change, 88-103. April 2019.
 
[20]  Carlile, M. J., Watkinson, S. C., & Gooday, G. W. The fungi. Gulf Professional Publishing. 2001.
 
[21]  Ayeloja, A. A., George, F. O. A., Jimoh, W. A., Shittu, M. O., & Abdulsalami, S. A. Microbial load on smoked fish commonly traded in Ibadan, Oyo State, Nigeria. Journal of Applied Sciences and Environmental Management, 22(4), 493-497. April 2018.
 
[22]  Cheesbrough, M. Microbiological test. In: District Laboratory Practice in Tropical Countries Part 2. Cambridge University Press, Cambridge Cape Town. 2002.