American Journal of Microbiological Research
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American Journal of Microbiological Research. 2013, 1(4), 98-104
DOI: 10.12691/ajmr-1-4-6
Open AccessArticle

Microbiological Profiling of Imported Apples and Demonstration of Bacterial Survival Capacity through in vitro Challenge Test

Nour Fatema1, Mrityunjoy Acharjee1 and Rashed Noor1,

1Department of Microbiology, Stamford University Bangladesh,Siddeswari Road, Dhaka, Bangladesh

Pub. Date: December 08, 2013

Cite this paper:
Nour Fatema, Mrityunjoy Acharjee and Rashed Noor. Microbiological Profiling of Imported Apples and Demonstration of Bacterial Survival Capacity through in vitro Challenge Test. American Journal of Microbiological Research. 2013; 1(4):98-104. doi: 10.12691/ajmr-1-4-6

Abstract

Present study conducted a complete investigation on microorganisms contaminating the imported apples commonly consumed in Dhaka metropolis, Bangladesh. Study of microbial prevalence demonstrated a substantial number of total viable bacteria (~107 cfu/g) in each of the dissected portions, i. e., surface, body and core of the samples except for the body and core portion of Malus paulared (Paula Red). Among the specific pathogens, Staphylococcus spp. was found to be predominant within a range of 1.6×105-2.8×107 cfu/g, while Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Pseudomonas spp., Vibrio spp. and Listeria spp. were also identified. Fungal contamination (~106 cfu/g) was observed only in the surface of each of the categories of apple samples tested. Study of antibiogram showed that most of the bacterial isolates were multi-drug resistant (MDR); however, study of antimicrobial activity showed that the Malus pumila (Golden delicious apple) samples possessed the anti-bacterial effect against Vibrio spp. Further studies employing the in vitro challenge test revealed a decline trend in the survival potential of spoiling bacteria in the apple samples tested.

Keywords:
apple food quality food safety microorganisms microbial challenge test

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