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American Journal of Medical and Biological Research. 2017, 5(2), 18-22
DOI: 10.12691/ajmbr-5-2-2
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Downregulation of MMP-9 Level and GCS Score Improvement in Severe Traumatic Brain Injury Due to the Mild Hypothermia Therapy

Eko Prasetyo1, , Andi Asadul Islam2, Mochammad Hatta3, Djoko Widodo2 and Ilhamjaya Pattelongi4

1Departement of Surgery, Division of Neurosurgery, Faculty of Medicine, University of Sam Ratulangi, Manado, Indonesia

2Departement of Surgery, Division of Neurosurgery, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

3Molecular Biology and Immunology Laboratory, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

4Departement of Physiology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

Pub. Date: September 09, 2017

Cite this paper:
Eko Prasetyo, Andi Asadul Islam, Mochammad Hatta, Djoko Widodo and Ilhamjaya Pattelongi. Downregulation of MMP-9 Level and GCS Score Improvement in Severe Traumatic Brain Injury Due to the Mild Hypothermia Therapy. American Journal of Medical and Biological Research. 2017; 5(2):18-22. doi: 10.12691/ajmbr-5-2-2


We recently investigated the effect of the mild hypothermia therapy towards alteration of matrix metalloproteinase-9 (MMP-9) and outcome of severe traumatic brain injury (TBI). The neurologic outcome was assessed by applying with GCS (Glasgow Coma Scale) score. Twenty patients with severe TBI whose the GCS score ≤ 8 between June 2015 and June 2016 were enrolled in RD Kandou Hospital, Manado, Indonesia. Patients were randomized into two groups, with and without the mild hypothermia therapy (34-36°C) which was investigated within 24 and 72 h. The MMP-9 level was estimated using enzyme-linked immunosorbent assay (ELISA). The assessments of outcomes were determined using the GCS score within 24 and 72 h during the mild hypothermia therapy. Our results showed that the level of serum MMP-9 was decreased significantly within 72 h in the mild hypothermia therapy group. The effects of the mild hypothermia therapy toward to the GCS score alteration were noticeable increased and differences were significant in the two groups within 72 h. We concluded that the mild hypothermia therapy diminished MMP-9 protein level and improvement of the GCS score in severe TBI patients within 72 h.

mild hypothermia MMP-9 GCS TBI

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